Human Protein C Inhibitor ELISA Kit | PCI elisa kit

Human Protein C Inhibitor ELISA Kit

Cat. Num. AAA726586

• Gene Names: SERPINA5; PCI; PAI3; PAI-3; PCI-B; PROCI; PLANH3
• Reactivity: Human
• Synonyms: Protein C Inhibitor; Human Protein C Inhibitor ELISA Kit; PCI elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This PCI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PCI. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCI elisa kit

Principle of the Assay: PCI ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PCI. Standards or samples are then added to the microtiter plate wells and PCI if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PCI present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PCI are added to each well to "sandwich" the PCI immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PCI and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCI concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PCI elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 546321
• NCBI GeneID: 5104
• Molecular Weight: 45,675 Da
• NCBI Official Full Name: protein C inhibitor
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• NCBI Official Symbol: SERPINA5
• NCBI Official Synonym Symbols: PCI; PAI3; PAI-3; PCI-B; PROCI; PLANH3
• NCBI Protein Information: plasma serine protease inhibitor; protein C inhibitor; plasminogen activator inhibitor-3; acrosomal serine protease inhibitor; plasminogen activator inhibitor III; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• UniProt Protein Name: Plasma serine protease inhibitor
• UniProt Gene Name: SERPINA5
• UniProt Synonym Gene Names: PCI; PLANH3; PROCI; PAI-3; PAI3; PCI
• UniProt Entry Name: IPSP_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. This gene is one in a cluster of serpin genes located on the q arm of chromosome 14. This family member is a glycoprotein that can inhibit several serine proteases, including protein C and various plasminogen activators and kallikreins, and it thus plays diverse roles in hemostasis and thrombosis in multiple organs. [provided by RefSeq, Aug 2012]

Uniprot Description

SERPINA5: Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary- type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue-and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as an hydrophobic hormone carrier by its binding to retinoic acid. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: extracellular space; protein complex; membrane; extracellular region; external side of plasma membrane; acrosomal membrane

Molecular Function: acrosin binding; heparin binding; serine-type endopeptidase inhibitor activity; glycosaminoglycan binding; retinoic acid binding; protease binding; phosphatidylcholine binding

Biological Process: fusion of sperm to egg plasma membrane; spermatogenesis; blood coagulation; lipid transport