SEMG1 recombinant protein

SEMG1, 24-462aa, Human, His tag, E Coli

Cat. Num. AAA205337

• Gene Names: SEMG1; SGI; SEMG; CT103; dJ172H20.2
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: SEMG1; 24-462aa; Human; His tag; E Coli; Semenogelin-1; Semenogelin 1; CT103; dJ172H20.2; SEMG; SGI; SEMG1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 7.5) containing 0.5M NaCl, 10% glycerol, 250mM Imidazole, 0.1mM PMSF, 1mM DTT.
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSQKGGSKG RLPSEFSQFP HGQKGQHYSG QKGKQQTESK GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL GGSQQLLHNK QEGRDHDKSK GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP SGKGISSQYS NTEERLWVHG LSKEQTSVSG AQKGRKQGGS QSSYVLQTEE LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP AHQDKLQHGS KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS SSTEERRLHY GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG ENAKGESGQS TNREQDLLSH EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA QHLNNDRNPL FT
• Sequence Length: 462

• Applicable Applications for SEMG1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SEMG1 recombinant protein

SEMG1 is the predominant protein in semen. The secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human SEMG1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SEMG1 recombinant protein

Signal Transduction

References

Brillard-Bourdet M, et al. (2002). Eur J Biochem. 269 (1):390-5

NCBI and Uniprot Product Information

• NCBI GI #: 4506883
• NCBI GeneID: 6406
• NCBI Accession #: NP_002998
• NCBI GenBank Nucleotide #: NM_003007.4
• UniProt Accession #: P04279; Q53ZV0; Q53ZV1; Q53ZV2; Q6X4I9; Q6Y809; Q6Y822; Q6Y823; Q86U64; Q96QM3
• Molecular Weight: 52kDa (462aa)
• NCBI Official Full Name: semenogelin-1 preproprotein
• NCBI Official Synonym Full Names: semenogelin 1
• NCBI Official Symbol: SEMG1
• NCBI Official Synonym Symbols: SGI; SEMG; CT103; dJ172H20.2
• NCBI Protein Information: semenogelin-1
• UniProt Protein Name: Semenogelin-1
• Protein Family: Semenogelin
• UniProt Gene Name: SEMG1
• UniProt Synonym Gene Names: SEMG; SGI

NCBI Description

The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. This preproprotein is proteolytically processed by the prostate-specific antigen (PSA) protease to generate multiple peptide products that exhibit distinct functions. One of these peptides, SgI-29, is an antimicrobial peptide with antibacterial activity. This proteolysis process also breaks down the gel matrix and allows the spermatozoa to move more freely. This gene and another similar semenogelin gene are present in a gene cluster on chromosome 20. [provided by RefSeq, Feb 2016]

Uniprot Description

Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.