Goat Semaphorin 7A ELISA Kit | SEMA7A elisa kit

Goat Semaphorin 7A ELISA Kit

Cat. Num. AAA740259

• Gene Names: SEMA7A; JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• Reactivity: Goat
• Synonyms: Semaphorin 7A; Goat Semaphorin 7A ELISA Kit; SEMA7A elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL
• Intended Uses: This SEMA7A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat SEMA7A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA7A elisa kit

Principle of the Assay: SEMA7A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SEMA7A antibody and an SEMA7A-HRP conjugate. The assay sample and buffer are incubated together with SEMA7A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SEMA7A concentration since SEMA7A from samples and SEMA7A-HRP conjugate compete for the anti-SEMA7A antibody binding site. Since the number of sites is limited, as more sites are occupied by SEMA7A from the sample, fewer sites are left to bind SEMA7A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEMA7A concentration in each sample is interpolated from this standard curve

Product Categories/Family for SEMA7A elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 225637524
• NCBI GeneID: 8482
• NCBI Accession #: NP_001139502.1
• NCBI GenBank Nucleotide #: NM_001146030.1
• UniProt Accession #: O75326; Q1XE81; Q1XE82; Q1XE83; Q1XE84; Q3MIY5
• Molecular Weight: 74,824 Da
• NCBI Official Full Name: semaphorin-7A isoform 3
• NCBI Official Synonym Full Names: semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• NCBI Official Symbol: SEMA7A
• NCBI Official Synonym Symbols: JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• NCBI Protein Information: semaphorin-7A; sema L; sema K1; semaphorin L; semaphorin-L; semaphorin K1; semaphorin-K1; JMH blood group antigen; John Milton Hagen blood group H-Sema K1; john-Milton-Hargen human blood group Ag; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group)
• UniProt Protein Name: Semaphorin-7A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA7A
• UniProt Synonym Gene Names: CD108; SEMAL; Sema K1; Sema L
• UniProt Entry Name: SEM7A_HUMAN

NCBI Description

The protein encoded by this gene binds to cell surfaces through a glycosylphosphatidylinositol (GPI) linkage. The encoded glycoprotein is found on activated lymphocytes and erythrocytes. This protein may be involved in immunomodulatory and neuronal processes. Defects in this gene can result in loss of bone mineral density (BMD). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Uniprot Description

SEMA7A: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. Belongs to the semaphorin family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 15q22.3-q23

Cellular Component: membrane; plasma membrane; external side of plasma membrane

Molecular Function: integrin binding; protein binding; receptor activity

Biological Process: integrin-mediated signaling pathway; osteoblast differentiation; axon guidance; axon extension; positive regulation of axon extension; regulation of inflammatory response; olfactory lobe development; immune response; positive regulation of protein amino acid phosphorylation; inflammatory response

Disease: Blood Group, John Milton Hagen System