Human Phospholamban (PLN) ELISA Kit | PLN elisa kit

Human Phospholamban (PLN) ELISA Kit

Cat. Num. AAA456911

• Gene Names: PLN; PLB; CMD1P; CMH18
• Reactivity: Human
• Synonyms: Phospholamban (PLN); Human Phospholamban (PLN) ELISA Kit; PLB; CMD1P; Cardiac phospholamban; PLN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLN. No significant cross-reactivity or interference between PLN and analogues was observed.
• Sequence Length: 52

• Samples: Tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.068ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLN elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PLN in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505887
• NCBI GeneID: 5350
• NCBI Accession #: NP_002658.1
• NCBI GenBank Nucleotide #: NM_002667.4
• UniProt Accession #: P26678
• Molecular Weight: 6,109 Da
• NCBI Official Full Name: cardiac phospholamban
• NCBI Official Synonym Full Names: phospholamban
• NCBI Official Symbol: PLN
• NCBI Official Synonym Symbols: PLB; CMD1P; CMH18
• NCBI Protein Information: cardiac phospholamban
• UniProt Protein Name: Cardiac phospholamban
• Protein Family: Cardiac phospholamban
• UniProt Gene Name: PLN
• UniProt Synonym Gene Names: PLB; PLB

NCBI Description

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. [provided by RefSeq, Apr 2016]

Uniprot Description

PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Protein type: Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q22.31

Cellular Component: calcium ion-transporting ATPase complex; endoplasmic reticulum; membrane; mitochondrial membrane; mitochondrion; perinuclear region of cytoplasm; protein complex; sarcoplasmic reticulum membrane; vesicle

Molecular Function: ATPase binding; ATPase inhibitor activity; calcium channel regulator activity; enzyme inhibitor activity; identical protein binding; protein binding

Biological Process: adrenergic receptor signaling pathway involved in heart process; blood circulation; calcium ion transport; cardiac muscle tissue development; negative regulation of ATPase activity; negative regulation of calcium ion import; negative regulation of calcium ion import into sarcoplasmic reticulum; negative regulation of calcium ion transmembrane transporter activity; negative regulation of calcium ion transport; negative regulation of catalytic activity; negative regulation of heart rate; Notch signaling pathway; protein homooligomerization; regulation of calcium ion transport; regulation of cardiac muscle cell contraction; regulation of cardiac muscle cell membrane potential; regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion; regulation of cytosolic calcium ion concentration; regulation of heart contraction; regulation of the force of heart contraction; regulation of the force of heart contraction by cardiac conduction; response to insulin stimulus; response to testosterone stimulus; response to zinc ion

Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18