SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
)
E-selectin/CD62E Recombinant Protein | CD62E recombinant protein
Recombinant human E-selectin/CD62E protein
Gene Names
SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
E-selectin/CD62E; Recombinant human E-selectin/CD62E protein; E-selectin; CD62E; ELAM; ELAM1; ELAM-1; ESEL; LECAM2; CD62 antigen-like family member E; endothelial-leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 2; SELE; CD62E antigen; ELAM1E-selectin; Endothelial leukocyte adhesion molecule 1; CD62E recombinant protein
Host
Baculovirus
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
22-556aa
Sequence
WSYNTSTEAM TYDEASAYCQ QRYTHLVAIQ NKEEIEYLNS ILSYSPSYYW IGIRKVNNVW VWVGTQKPLT EEAKNWAPGE PNNRQKDEDC VEIYIKREKD VGMWNDERCS KKKLALCYTA ACTNTSCSGH GECVETINNY TCKCDPGFSG LKCEQIVNCT ALESPEHGSL VCSHPLGNFS YNSSCSISCD RGYLPSSMET MQCMSSGEWS APIPACNVVE CDAVTNPANG FVECFQNPGS FPWNTTCTFD CEEGFELMGA QSLQCTSSGN WDNEKPTCKA VTCRAVRQPQ NGSVRCSHSP AGEFTFKSSC NFTCEEGFML QGPAQVECTT QGQWTQQIPV CEAFQCTALS NPERGYMNCL PSASGSFRYG SSCEFSCEQG FVLKGSKRLQ CGPTGEWDNE KPTCEAVRCD AVHQPPKGLV RCAHSPIGEF TYKSSCAFSC EEGFELHGST QLECTSQGQW TEEVPSCQVV KCSSLAVPGK INMSCSGEPV FGTVCKFACP EGWTLNGSAA RTCGATGHWS GLLPTCEAPT ESNIP
Applicable Applications for CD62E recombinant protein
SDS-PAGE, Bioactivity
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Human
Tag
His-Tag
Predicted N-term
Trp 22
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to human E-Seletin/CD62E coated plates 2ug/ml. This effect is more to 40%.
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
)
Related Product Information for CD62E recombinant protein
E-selectin, also known as CD62 antigen-like family member E (CD62E), is a member of the Selectin family. It is a selectin cell adhesion molecule expressed only on endothelial cells activated by cytokines. Like other selectins, it plays an important part in inflammation. The local release of cytokines IL-1 and TNF-alpha by Macrophages in the inflamed tissue induces the over-expression of E-selectin on endothelial cells of nearby blood vessels. As the inflammatory response progresses, chemokines released by injured tissue enter the blood vessels and activate the rolling leukocytes, which are now able to tightly bind to the endothelial surface and begin making their way into the tissue. While numerous pieces of in vitro and clinical evidence continue to support this hypothesis of E-selectin-mediated cancer metastasis, in vivo studies of cancer metastasis have shown that E-selectin knockout only minimally affects leukemic cell adhesion to bone immediately following injection. Recombinant human E-selectin, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for CD62E recombinant protein
References
Collins T, et al., (1991), J. Biol. Chem. 266:2466-2473. Kawase J, et al. (2009). Oncol Rep. 22:1293-1297. Matsumoto K, et al. (2010). Metabolism. 59:320-324.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,655 Da
NCBI Official Full Name
E-selectin
NCBI Official Synonym Full Names
selectin E
NCBI Official Symbol
SELE
NCBI Official Synonym Symbols
ELAM; ESEL; CD62E; ELAM1; LECAM2
NCBI Protein Information
E-selectin; CD62 antigen-like family member E; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2
UniProt Protein Name
E-selectin
UniProt Gene Name
SELE
UniProt Synonym Gene Names
ELAM1; ELAM-1; LECAM2
UniProt Entry Name
LYAM2_HUMAN
Uniprot Description
SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q22-q25
Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; integral to membrane; plasma membrane; coated pit; caveola; lipid raft
Molecular Function: protein binding; phospholipase binding; transmembrane receptor activity; sialic acid binding
Biological Process: heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; positive regulation of leukocyte migration; actin filament-based process; positive regulation of receptor internalization; regulation of inflammatory response; calcium-mediated signaling; response to lipopolysaccharide; blood coagulation; leukocyte tethering or rolling; inflammatory response; leukocyte migration; leukocyte migration during inflammatory response
Disease: Hypertension, Essential
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Product Notes
The CD62E sele (Catalog #AAA201307) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 22-556aa. AAA Biotech's E-selectin/CD62E can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the CD62E sele for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: WSYNTSTEAM TYDEASAYCQ QRYTHLVAIQ NKEEIEYLNS ILSYSPSYYW IGIRKVNNVW VWVGTQKPLT EEAKNWAPGE PNNRQKDEDC VEIYIKREKD VGMWNDERCS KKKLALCYTA ACTNTSCSGH GECVETINNY TCKCDPGFSG LKCEQIVNCT ALESPEHGSL VCSHPLGNFS YNSSCSISCD RGYLPSSMET MQCMSSGEWS APIPACNVVE CDAVTNPANG FVECFQNPGS FPWNTTCTFD CEEGFELMGA QSLQCTSSGN WDNEKPTCKA VTCRAVRQPQ NGSVRCSHSP AGEFTFKSSC NFTCEEGFML QGPAQVECTT QGQWTQQIPV CEAFQCTALS NPERGYMNCL PSASGSFRYG SSCEFSCEQG FVLKGSKRLQ CGPTGEWDNE KPTCEAVRCD AVHQPPKGLV RCAHSPIGEF TYKSSCAFSC EEGFELHGST QLECTSQGQW TEEVPSCQVV KCSSLAVPGK INMSCSGEPV FGTVCKFACP EGWTLNGSAA RTCGATGHWS GLLPTCEAPT ESNIPPrecautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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