Rat sE-Selectin ELISA Kit | SELE elisa kit

Rat sE-Selectin PicoKine ELISA Kit

Cat. Num. AAA176526

• Reactivity: Rat
• Synonyms: sE-Selectin; Rat sE-Selectin PicoKine ELISA Kit; E-selectin; selectin E; CD62 antigen-like family member E; CD62E; CD62E antigen; E selectin; ELAM; ELAM-1; ELAM1; Endothelial leukocyte adhesion molecule 1; ESEL; LECAM2; Leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; LYAM2_HUMAN; Sele; Selectin E; SELE elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat sE-Selectin
• Sequence Length: 549

• Samples: Cell culture supernates, serum and plasma (heparin, citrate)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Rat sE-Selectin PicoKine ELISA Kit standard curve )

Related Product Information for SELE elisa kit

Principle of the assay: rat E-Selectin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for E-Selectin has been precoated onto 96-well plates. Standards(NSO, W22-P494) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat E-Selectin amount of sample captured in plate.
Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increasedexpression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homingof T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM).3 A structurallyand functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4

NCBI and Uniprot Product Information

• NCBI GI #: 1346437
• NCBI GeneID: 25544
• UniProt Accession #: P98105
• Molecular Weight: 60,080 Da
• NCBI Official Full Name: E-selectin
• NCBI Official Synonym Full Names: selectin E
• NCBI Official Symbol: Sele
• NCBI Protein Information: E-selectin; ELAM-1; LECAM2; selectin, endothelial cell; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte-endothelial cell adhesion molecule 2
• UniProt Protein Name: E-selectin
• Protein Family: E-selectin
• UniProt Gene Name: Sele
• UniProt Synonym Gene Names: Elam-1; ELAM-1; LECAM2
• UniProt Entry Name: LYAM2_RAT

NCBI Description

facilitates recruitment of leukocytes into sites of inflammation; plays a role in cell adhesion [RGD, Feb 2006]

Uniprot Description

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis ().