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603 results for " innate immunity" - showing 1-50
Activity Data
(Protein Kinase R (PKR) is activated by double-stranded RNA (dsRNA), the synthesis of which is caused virally. PKR can also be activated by the protein PACT or by heparin. It plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Besides, Cyclin Dependent Kinase 1 (CDK1) has been identified as an interactor of PKR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PKR and recombinant human CDK1. Briefly, PKR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDK1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PKR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of PKR and CDK1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Protein Kinase R (PKR), Active Protein (Cat# AAA2097276)
Full Name
Active Protein Kinase R (PKR)
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 80%
Pricing
$265/0.01 mg | $585/0.05 mg | $915/0.1 mg | $1,125/0.2 mg | $2,215/0.5 mg | $3,280/1 mg | $8,035/5 mg
GAPDH, Polyclonal Antibody (Cat# AAA9205368)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
CD282, Monoclonal Antibody (Cat# AAA439465)
Full Name
CD282 (Toll-Like Receptor 2) Mouse Monoclonal Antibody
Gene Names
TLR2; TIL4; CD282
Reactivity
Human, Monkey, Dog
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Figure 1. Western blot analysis of RANK using anti- RANK antibody (MBS1750556).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: HEPG2 whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- RANK antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RANK at approximately 80KD. The expected band size for RANK is at 66KD. )
RANK, Polyclonal Antibody (Cat# AAA1750556)
Full Name
Anti-RANK Picoband Antibody
Gene Names
TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot detection of BTK in Daudi, Jurkat (BTK negative), K562, Ramos and Raji cell lysates using BTK mouse mAb (1:1000 diluted).Predicted band size:77KDa.Observed band size:77KDa.)
BTK, Monoclonal Antibody (Cat# AAA475180)
Full Name
Anti-BTK Mouse mAb
Gene Names
BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
Reactivity
Human
Applications
WB, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Activity Data
(The Interferon-alpha (IFNa) proteins belong to type I interferons (IFNs) which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins produced by leukocytes are also known as leukocyte interferon. They are mainly involved in innate immune response against viral infection. Besides, Interferon-alpha 13 (IFNA13) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IFNa and recombinant mouse IFNA13. Briefly, IFNA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNA13-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IFNapAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNA13 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interferon Alpha (IFNa), Active Protein (Cat# AAA2097256)
Full Name
Active Interferon Alpha (IFNa)
Gene Names
Ifna1; Ifa1
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Immunohistochemistry (IHC)
(MBS9200563 staining CSF1R in human skin sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.)
CSF1R, Monoclonal Antibody (Cat# AAA9200563)
Full Name
CSF1R Antibody
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000281)
Full Name
Cyclooxygenase 1 Antibody: RPE
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Activity Data
(Figure. The binding activity of MIF with MHCDG.Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MIF and recombinant mouse MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105113)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
Mif; GIF; DER6; Glif
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Western Blot (WB)
(Leptin Receptor (LEPR) Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow).)
Leptin Receptor (LEPR), Polyclonal Antibody (Cat# AAA9204850)
Full Name
Leptin Receptor (LEPR) Antibody (N-term)
Gene Names
LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Figure 1. Western blot analysis of IL17 using anti-IL17 antibody (MBS1750396).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD. )
IL17, Polyclonal Antibody (Cat# AAA1750396)
Full Name
Anti-IL17 Picoband Antibody
Gene Names
Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
CD284, Monoclonal Antibody (Cat# AAA438978)
Full Name
CD284 (Toll-Like Receptor 4) Mouse Monoclonal Antibody
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Reactivity
Human, Monkey, Pig, Dog, Rat, Guinea Pig
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot detection of PI3 Kinase p85 beta in C6, K562, MCF7, U87MG, HCT116, 3T3 and Hela cell lysates using PI3 Kinase p85 beta mouse mAb (1:1000 diluted).Predicted band size:85KDa.Observed band size:85KDa.)
PI3 Kinase p85 beta, Monoclonal Antibody (Cat# AAA475225)
Full Name
Anti-PI3 Kinase p85 beta Mouse mAb
Gene Names
PIK3R2; p85; MPPH; P85B; MPPH1; p85-BETA
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
Myeloperoxidase, Polyclonal Antibody (Cat# AAA540041)
Full Name
Myeloperoxidase Antibody
Reactivity
Human, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Activity Data
(Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands. LTF is known to activate immune cells to produce several cytokines, such as tumor necrosis factor TNF-a, thus, a stimulation assay was conducted to detect the activity of LTF using human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into wells of 24-well plates at a density of 5×106 cells/mL in RPMI-1640 with the addition of various concentrations of recombinant human LTF. After incubation for 20 hours, the concentration of TNF-a in the cell supernatant was detected using an ELISA kit. TNF-a levels in the cell supernatant of spleen cells increased significantly after stimulated with LTF, the data was shown in table 1.)
Lactoferrin (LTF), Active Protein (Cat# AAA2105115)
Full Name
Active Lactoferrin (LTF)
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Applications
WB
Purity
>95%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Activity Data
(Figure. The binding activity of CD14 with LBP.Cluster Of Differentiation 14 (CD14), also known as CD14, is a component of the innate immune system. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns such as lipoteichoic acid. Besides, Lipopolysaccharide Binding Protein (LBP) has been identified as an interactor of CD14, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CD14 and recombinant human LBP. Briefly, CD14 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to LBP-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD14 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CD14 and LBP was shown in Figure 1, and this effect was in a dose dependent manner.)
Cluster Of Differentiation 14 (CD14), Active Protein (Cat# AAA2105110)
Full Name
Active Cluster Of Differentiation 14 (CD14)
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$245/0.01 mg | $530/0.05 mg | $820/0.1 mg | $1,015/0.2 mg | $1,985/0.5 mg | $2,935/1 mg | $7,180/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.)
RIP3, Polyclonal Antibody (Cat# AAA809969)
Full Name
RIP3 Antibody: Biotin
Gene Names
RIPK3; RIP3
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Activity Data
(Figure. The binding activity of MIF with MHCDG.Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIF and recombinant human MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105112)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
MIF; GIF; GLIF; MMIF
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $510/0.05 mg | $790/0.1 mg | $975/0.2 mg | $1,905/0.5 mg | $2,820/1 mg | $6,895/5 mg
Western Blot (WB)
(Western blot analysis of COLEC11 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). COLEC11 (arrow) was detected using the purified Pab.)
COLEC11, Polyclonal Antibody (Cat# AAA9214722)
Full Name
COLEC11 Antibody (N-term)
Gene Names
COLEC11; 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
CD163, Polyclonal Antibody (Cat# AAA540877)
Full Name
CD163 Antibody
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human, Monkey
Applications
EIA, IHC, IF, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (MBS1750500).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD. )
PF4/Cxcl4, Polyclonal Antibody (Cat# AAA1750500)
Full Name
Anti-PF4/Cxcl4 Picoband antibody
Gene Names
Pf4; PF-4; Pf4a; Cxcl4; RATPF4A
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Fig-2: Immunohistochemical analysis of TLR2 in human prostate tissue using TLR2 antibody (Clone: ABM3A87) at 5 ug/ml.)
TLR2, Monoclonal Antibody (Cat# AAA668966)
Full Name
NALE Monoclonal Antibody to TLR2 (Clone: ABM3A87) (No Azide Low Endotoxin)
Gene Names
TLR2; TIL4; CD282
Reactivity
Mouse, Human
Applications
IHC, WB, FC/FACS
Purity
Azide Free, Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $1,405/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of SKBR-3 cells using B7-H4 Mouse Monoclonal Antibody (B7H4/1788) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
B7-H4, Monoclonal Antibody (Cat# AAA4381352)
Full Name
B7-H4 (Immuno-Inhibitory Protein)
Gene Names
VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
Reactivity
Human
Applications
EIA, FC/FACS, IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of anti-CSF1R Pab in human placenta. CSF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA9211301)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000267)
Full Name
Cyclooxygenase 1 Antibody: ATTO 488
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(The anti-HK1 Pab is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.)
HK1 (Hexokinase), Polyclonal Antibody (Cat# AAA9204225)
Full Name
HK1 (Hexokinase) Antibody (N-term)
Gene Names
HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
Reactivity
Human, Rat
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of anti-IRF5 Antibody (N-term) MBS9213786 in Ramos cell line lysates (35ug/lane). IRF5 (arrow) was detected using the purified Pab.)
IRF5, Polyclonal Antibody (Cat# AAA9213786)
Full Name
IRF5 Antibody (N-term)
Gene Names
IRF5; SLEB10
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IF, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000280)
Full Name
Cyclooxygenase 1 Antibody: PerCP
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot detection of JNK1 in C6 and 3T3 cell lysates using JNK1 mouse mAb (1:1000 diluted).Predicted band size:46, 54KDa.Observed band size:46KDa.)
JNK1, Monoclonal Antibody (Cat# AAA475153)
Full Name
Anti-JNK1 Mouse mAb
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Activity Data
(Figure. The binding activity of IFNa with IFNa/bR1.Interferon Alpha (IFNa) belongs to a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins are produced by leukocytes. They are mainly involved in innate immune response against viral infection. It is also made synthetically as medication in hairy cell leukemia. Besides, Interferon Alpha/Beta Receptor 1 (IFNa/bR1) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat IFNa and recombinant rat IFNa/bR1. Briefly, IFNa were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNa/bR1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IFNa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNa/bR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interferon Alpha (IFNa), Active Protein (Cat# AAA2105053)
Full Name
Active Interferon Alpha (IFNa)
Gene Names
Ifna1; IFN-alpha1
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$190/0.01 mg | $385/0.05 mg | $590/0.1 mg | $725/0.2 mg | $1,405/0.5 mg | $2,095/1 mg | $5,145/5 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000265)
Full Name
Cyclooxygenase 1 Antibody
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809868)
Full Name
PI 3 Kinase Class 3 antibody
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with B7-H4 Rabbit Recombinant Monoclonal Antibody (B7H4/2652R).)
B7-H4, Monoclonal Antibody (Cat# AAA4380722)
Full Name
B7-H4 (Immuno-Inhibitory Protein)
Gene Names
VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
Reactivity
Human. Others not known.
Applications
EIA, Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
SDS-Page
(SDS-PAGE Analysis Purified Complement 3d Mouse Monoclonal Antibody (C3D/2891). Confirmation of Integrity and Purity of Antibody.)
Complement 3d (C3d), Monoclonal Antibody (Cat# AAA4381315)
Full Name
Complement 3d (C3d) (Acute Humoral Rejection Marker)
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809884)
Full Name
PI 3 Kinase Class 3 antibody: RPE
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809881)
Full Name
PI 3 Kinase Class 3 antibody: HRP
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (MBS1750574).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD40L at approximately 36KD. The expected band size for CD40L is at 29KD. )
CD40L, Polyclonal Antibody (Cat# AAA1750574)
Full Name
Anti-CD40L Picoband antibody
Gene Names
Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of SAMHD1 (phospho Thr592) in 293 cells transfected with (A) empty expression vector, (B) wild-type SAMHD1, (C) SAMHD1 (T592A) and (D) SAMHD1 (T592E) with SAMHD1 (phospho Thr592) antibody at 1 μg/ml.)
SAMHD1, Polyclonal Antibody (Cat# AAA154446)
Full Name
SAMHD1 (phospho Thr592) Antibody
Gene Names
SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
Affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Immunofluorescence (IF)
CD163, Polyclonal Antibody (Cat# AAA540774)
Full Name
CD163 Antibody C-epitope
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human, Monkey
Applications
EIA, IF, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB)
(Fig-1: Western blot analysis of TLR9. Anti- TLR9 antibody (Clone: ABM1C51) was used at 2 ug/ml on (1) Raji and (2) EL4 lysates.)
TLR9, Monoclonal Antibody (Cat# AAA668950)
Full Name
NALE Monoclonal Antibody to TLR9 (Clone: ABM1C51) (No Azide Low Endotoxin)
Gene Names
TLR9; CD289
Reactivity
Mouse, Human
Applications
IHC, WB, FC/FACS
Purity
Azide Free, Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $1,405/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD14 using anti-CD14 antibody (MBS1750332).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse thymus tissue lysates,Lane 2: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD14 at approximately 50KD. The expected band size for CD14 is at 40KD. )
CD14, Polyclonal Antibody (Cat# AAA1750332)
Full Name
Anti-CD14 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000013)
Full Name
IRGM Antibody
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB)
TLR10, Polyclonal Antibody (Cat# AAA540461)
Full Name
TLR10 Antibody
Gene Names
TLR10; CD290
Reactivity
Chimpanzee, Human, Monkey, Pig, Rat
Applications
EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000276)
Full Name
Cyclooxygenase 1 Antibody: Biotin
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000014)
Full Name
IRGM Antibody: ATTO 390
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)
IRGM, Polyclonal Antibody (Cat# AAA8000029)
Full Name
IRGM Antibody: RPE
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Flow Cytometry (FC/FACS)
(Fig-1: Immunohistochemical analysis of TLR7 in human Kidney tissue using TLR7 antibody (Clone: ABM2C27) at 5 ug/ml.)
TLR7, Monoclonal Antibody (Cat# AAA668962)
Full Name
NALE Monoclonal Antibody to TLR7 (Clone: ABM2C27) (No Azide Low Endotoxin)
Gene Names
TLR7; TLR7-like
Reactivity
Human
Applications
IHC, FC/FACS
Purity
Azide Free, Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $1,405/5x0.1 mg
Western Blot (WB)
TLR1, Polyclonal Antibody (Cat# AAA540395)
Full Name
TLR1 Antibody
Reactivity
Mouse, Rat
Predicted: Human
Predicted: Human
Applications
EIA, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.)
PI 3 Kinase Class 3, Polyclonal Antibody (Cat# AAA809872)
Full Name
PI 3 Kinase Class 3 antibody: ATTO 594
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Activity Data
(Figure. The binding activity of IL33 with IL1RL1.Interleukin 33 is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines. IL33 is a ligand for ST2 (IL1RL1), an IL-1 family receptor that is highly expressed on Th2 cells, mast cells and group 2 innate lymphocytes. IL-33 is expressed by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. Besides, Interleukin 1 Receptor Like Protein 1 (IL1RL1) has been identified as an interactor of IL33, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL33 and recombinant human IL1RL1. Briefly, IL33 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1RL1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL33pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL33and IL1RL1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 33 (IL33), Active Protein (Cat# AAA2105157)
Full Name
Active Interleukin 33 (IL33)
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$240/0.01 mg | $515/0.05 mg | $800/0.1 mg | $990/0.2 mg | $1,935/0.5 mg | $2,865/1 mg | $7,010/5 mg
