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15 results for " Over Expression Lysates" - showing 1-15
Western Blot (WB)
(Figure 1 Western Blot Validation of PD-L1 in HeLa CellsLoading: 15ug of lysates per lane. Antibodies: MBS151123 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
PDL-1, Polyclonal Antibody (Cat# AAA151123)
Full Name
PDL-1 Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC-P, IF, FC
Purity
PD-L1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Immunofluorescence (IF)
(MBS9606617 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(DF5165) and mouse anti-beta tubulin Ab (MBS9610328) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
TAS2R5, Polyclonal Antibody (Cat# AAA9606617)
Full Name
TAS2R5 Antibody
Gene Names
TAS2R5; T2R5
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
Peptide affinity purifiaction
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9601042 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Merlin, Polyclonal Antibody (Cat# AAA9601042)
Full Name
Phospho-Merlin (Ser518) Antibody
Gene Names
NF2; ACN; SCH; BANF
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9601852 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Merlin, Polyclonal Antibody (Cat# AAA9601852)
Full Name
Merlin Antibody
Gene Names
NF2; ACN; SCH; BANF
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data #1
(Western blot analysis of whole cell lysate from EL4 mouse lymphoblast cells probed with Rabbit anti Human TSC1 (MBS221310) in the absence (A) or presence (B) of blocking peptide)
TSC1, Polyclonal Antibody (Cat# AAA221310)
Full Name
RABBIT ANTI TSC1
Gene Names
TSC1; LAM; TSC
Reactivity
Mouse, Rat
Applications
WB
Pricing
$390/0.1 mg | $1,605/5x0.1 mg
Testing Data
(Total RNA was analyzed by electrophoresis on 1% agarose gel, using T5595-9721)
Tissue, Universal RNA, Monkey, RNA Extraction (Cat# AAA638650)
Full Name
Tissue, Universal RNA, Monkey (Cynomolgus & Rhesus) Adult Normal
Purity
Molecular Biology Grade
Pricing
$670/2x0.1 mg | $995/4x0.1 mg
Western Blot (WB)
(Western blot analysis of lysate from LNCaP cell line, using JAK2 Antibody (C-term). MBS9213347 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
JAK2, Polyclonal Antibody (Cat# AAA9213347)
Full Name
JAK2 Antibody (C-term)
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysate from HT-29 cell line, using ERN2 Antibody (N-term). MBS9210486 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
ERN2, Polyclonal Antibody (Cat# AAA9210486)
Full Name
ERN2 Antibody (N-term)
Gene Names
ERN2; IRE1b; IRE2p; hIRE2p; IRE1-BETA
Reactivity
Human
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
Tissue, Universal RNA Adult Normal, RNA Extraction (Cat# AAA638544)
Full Name
Tissue, Universal RNA, Mouse Adult Normal
Purity
Molecular Biology Grade
Pricing
$590/2x0.1 mg | $890/4x0.1 mg
Tissue, Universal RNA Adult Normal, RNA Extraction (Cat# AAA638528)
Full Name
Tissue, Universal RNA, Human Adult Normal
Purity
Molecular Biology Grade
Pricing
$670/2x0.1 mg | $995/4x0.1 mg
Western Blot (WB)
(Western blot analysis of MAP LC3beta expression in non-transfected 293T (A), human MAP LC3beta transfected 293T (B) and IM R-32 (C) whole cell lysates..)
LC3B, Polyclonal Antibody (Cat# AAA9435173)
Full Name
LC3B Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, IHC-P
Purity
Immunogen affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Tissue, Universal RNA, Canine Adult Normal, RNA Extraction (Cat# AAA638706)
Full Name
Tissue, Universal RNA, Canine Adult Normal
Purity
Molecular Biology Grade
Pricing
$670/2x0.1 mg | $995/4x0.1 mg
Tissue, Universal RNA, Rat Adult Normal, RNA Extraction (Cat# AAA638655)
Full Name
Tissue, Universal RNA, Rat Adult Normal
Purity
Molecular Biology Grade
Pricing
$590/2x0.1 mg | $890/4x0.1 mg
Western Blot (WB)
(Western blot detection of Phospho-AKT (Ser473) in C6,3T3,Hela cell lysates using Phospho-AKT (Ser473) Rabbit mAb(1:1000 diluted).Predicted band size:56kDa.Observed band size:56kDa.)
AKT, Monoclonal Antibody (Cat# AAA4754090)
Full Name
Phospho-AKT (Ser473) Rabbit mAb
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IHC-F, ICC, IF
Purity
Affinity purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with DYKDDDDK-GSK3B protein, using DDDDK-Tag antibody at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
DDDDK-Tag, Monoclonal Antibody (Cat# AAA9143958)
Full Name
DDDDK-Tag Rabbit mAb
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
$210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
