Tissue, Universal RNA Adult Normal RNA Extraction
Tissue, Universal RNA, Human Adult Normal
Purity
Molecular Biology Grade
Synonyms
Tissue; Universal RNA Adult Normal; Universal RNA; Human Adult Normal; Universal RNA Adult Normal rna extraction
Purity/Purification
Molecular Biology Grade
Quality Control
1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-HCl, pH 7.5).
2. The Universal RNA is analyzed by capillary electrophoresis with Agilent 2100 bioanalyzer. RNA integrity is determined with RIN (RNA Integrity Number) 7, and the area of 28s over 18s ratio 1.
3. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR.
4. cDNA synthesis is successfully performed by using this RNA as template.
5. Universal RNA is labeled and hybridized to a cDNA microarray, expected result of gene expression is consistent with each lot.
2. The Universal RNA is analyzed by capillary electrophoresis with Agilent 2100 bioanalyzer. RNA integrity is determined with RIN (RNA Integrity Number) 7, and the area of 28s over 18s ratio 1.
3. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR.
4. cDNA synthesis is successfully performed by using this RNA as template.
5. Universal RNA is labeled and hybridized to a cDNA microarray, expected result of gene expression is consistent with each lot.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Tissue, Universal RNA Adult Normal rna extraction
Human Universal RNA is prepared from major organs of both male and female adult donors.
This total RNA serves as a standard for comparison of gene expression from microarray
studies.
This total RNA serves as a standard for comparison of gene expression from microarray
studies.
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Product Notes
The Tissue, Universal RNA Adult Normal (Catalog #AAA638528) is a RNA Extraction and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tissue, Universal RNA Adult Normal, RNA Extraction" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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