Rabbit anti-Mouse, Rat TSC1 Polyclonal Antibody | anti-TSC1 antibody

RABBIT ANTI TSC1

Cat. Num. AAA221310

• Gene Names: TSC1; LAM; TSC
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: TSC1; Polyclonal Antibody; RABBIT ANTI TSC1; anti-TSC1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises an epitope within the internal region of the 130kDa tumour suppressor protein, Tumour sclerosis complex 1 (TSC1). Mutation within the TSC1 gene is responsible for Tuberous sclerosis (TSC), an autosomal dominant genetic disorder associated with the development of benign tumors (hamartomas), a condition also induced by mutation of the Tumour sclerosis complex 2 (TSC2) gene. ; Many proteins have been shown to play a role in the regulation of the serine/threonine kinase known as target of rapamycin (TOR), a central component in a complex signaling pathway which controls cell proliferation and cell cycle progression. Negative regulation of TOR activity occurs following the over-expression of the TSC1 and TSC2 gene products, hamartin and tuberin. These act by suppressing the phosphorylation of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) and ribosomal protein S6 kinase (S6K), downstream targets of TOR involved in mRNA translation. Furthermore the TOR activator protein Rheb has been identified as a target of TSC1-TSC2 complexes. ; ; This item has been reported as suitable for use in IHC on mouse EL4 cell cytospins at 2ug/ml.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1,113

• Applicable Applications for anti-TSC1 antibody: Immunohistology Frozen, Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 130kDa in mouse EL4 cell lysates.; Western Blotting: Maximum Dilution: 1ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: A 15 amino acid peptide located near the centre of human TSC1.; Buffer Solution
• Antiserum Preparation: Antisera to human TSC1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from EL4 mouse lymphoblast cells probed with Rabbit anti Human TSC1 (MBS221310) in the absence (A) or presence (B) of blocking peptide)

Testing Data #2

(Immunocytochemical staining of EL4 cells with Rabbit anti Human TSC1 (MBS221310))

Related Product Information for anti-TSC1 antibody

MBS221310 recognises an epitope within the internal region of the 130kDa tumour suppressor protein, Tumour sclerosis complex 1 (TSC1). Mutation within the TSC1 gene is responsible for Tuberous sclerosis (TSC), an autosomal dominant genetic disorder associated with the development of benign tumors (hamartomas), a condition also induced by mutation of the Tumour sclerosis complex 2 (TSC2) gene. Many proteins have been shown to play a role in the regulation of the serine/threonine kinase known as target of rapamycin (TOR), a central component in a complex signaling pathway which controls cell proliferation and cell cycle progression. Negative regulation of TOR activity occurs following the over-expression of the TSC1 and TSC2 gene products, hamartin and tuberin. These act by suppressing the phosphorylation of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) and ribosomal protein S6 kinase (S6K), downstream targets of TOR involved in mRNA translation. Furthermore the TOR activator protein Rheb has been identified as a target of TSC1-TSC2 complexes. MBS221310 has been reported as suitable for use in IHC on mouse EL4 cell cytospins at 2ug/ml.

NCBI and Uniprot Product Information

• NCBI GI #: 4507693
• NCBI GeneID: 7248
• NCBI Accession #: NP_000359.1
• NCBI GenBank Nucleotide #: NM_000368.4
• UniProt Accession #: Q92574; Q5VVN5; B7Z897
• Molecular Weight: 124,015 Da
• NCBI Official Full Name: hamartin isoform 1
• NCBI Official Synonym Full Names: tuberous sclerosis 1
• NCBI Official Symbol: TSC1
• NCBI Official Synonym Symbols: LAM; TSC
• NCBI Protein Information: hamartin; tuberous sclerosis 1 protein; tumor suppressor
• UniProt Protein Name: Hamartin
• Protein Family: Tuberous sclerosis 1 protein
• UniProt Gene Name: TSC1
• UniProt Synonym Gene Names: KIAA0243; TSC
• UniProt Entry Name: TSC1_HUMAN

NCBI Description

This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]

Uniprot Description

TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: TSC1-TSC2 complex; protein complex; growth cone; intracellular membrane-bound organelle; membrane; lamellipodium; cytoplasm; plasma membrane; actin filament; cell cortex; cytosol

Molecular Function: protein binding; chaperone binding; protein N-terminus binding; GTPase activating protein binding; GTPase regulator activity

Biological Process: myelination; regulation of cell-matrix adhesion; regulation of cell cycle; protein heterooligomerization; cell-matrix adhesion; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; regulation of phosphoprotein phosphatase activity; cardiac muscle cell differentiation; response to insulin stimulus; regulation of translation; negative regulation of cell proliferation; positive regulation of focal adhesion formation; cell projection organization and biogenesis; synapse organization and biogenesis; cell cycle arrest; kidney development; potassium ion transport; negative regulation of TOR signaling pathway; protein stabilization; hippocampus development; rRNA export from nucleus; negative regulation of translation; glucose import; regulation of stress fiber formation; neural tube closure; insulin receptor signaling pathway; cerebral cortex development; regulation of protein kinase activity

Disease: Lymphangioleiomyomatosis; Focal Cortical Dysplasia Of Taylor; Tuberous Sclerosis 1