Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "cell-cycle-protein" - showing 4550-4600
Typical Testing Data/Standard Curve (for reference only)
BMP-6, ELISA Kit (Cat# AAA1751130)
Full Name
Mouse BMP-6 PicoKine ELISA Kit
Gene Names
Bmp6; Vgr1; D13Wsu115e
Reactivity
Mouse
There is no detectable cross-reactivity with other relevant proteins.
There is no detectable cross-reactivity with other relevant proteins.
Applications
EIA
Pricing
$460/96-Strip-Wells | $2,060/5x96-Strip-Wells
Western Blot (WB)
(Western blot analysis on HuvEc cell lysate using Phospho-Nibrin (Ser278) Antibody)
Nibrin, Antibody (Cat# AAA8511137)
Full Name
Phospho-Nibrin (Ser278) Antibody
Gene Names
NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2
Reactivity
Human
Applications
WB, IF, ICC
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
P2R3C, Blocking Peptide (Cat# AAA9228374)
Full Name
P2R3C Antibody (N-term) Blocking Peptide
Gene Names
PPP2R3C; G4-1; G5pr; C14orf10
Sgk1, Blocking Peptide (Cat# AAA9223850)
Full Name
Mouse Sgk1 Antibody (C-term) Blocking Peptide
Gene Names
Sgk1; Sgk
RAD17, Blocking Peptide (Cat# AAA9218572)
Full Name
RAD17 Antibody (Center) Blocking peptide
Gene Names
RAD17; CCYC; R24L; RAD24; HRAD17; RAD17SP
Immunofluorescence (IF)
(Immunofluorescence analysis of HUVEC cells, using CCRK Antibody. The picture on the right is blocked with the synthesized peptide.)
CCRK, Polyclonal Antibody (Cat# AAA9505334)
Full Name
CCRK Antibody
Gene Names
CDK20; P42; CCRK; CDCH; PNQALRE
Reactivity
Human, Mouse, Rat
Applications
WB, IF, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
CDK1 (T14), Blocking Peptide (Cat# AAA9226962)
Full Name
CDK1 Antibody (T14) Blocking Peptide
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
CAMK1b, Recombinant Protein (Cat# AAA5303985)
Full Name
CAMK1b protein
Purity
CAMK1b protein was purified using a a proprietary chromatographic technique.
Pricing
$555/0.005 mg | $2,490/5x0.005 mg
Western Blot (WB)
(Western blot analysis of Mouse kidney lysate showing detection of ~95.6 kDa Axin 1 protein using Rabbit Anti-Axin 1 Polyclonal Antibody (SPC-721). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse kidney lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody (SPC-721) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95.6 kDa.)
Axin 1, Polyclonal Antibody (Cat# AAA8000420)
Full Name
Axin 1 Antibody: Biotin
Gene Names
AXIN1; AXIN; PPP1R49
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Collagen Type VI, Polyclonal Antibody (Cat# AAA2033813)
Full Name
Polyclonal Antibody to Collagen Type VI (COL6)
Gene Names
CDC123; D123; C10orf7
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$175/0.1 mL | $220/0.2 mL | $375/0.5 mL | $460/1 mL | $1,290/5x1 mL
RGCC, siRNA (Cat# AAA8233134)
Full Name
RGCC siRNA (Rat)
Gene Names
Rgcc; Rgc32
Reactivity
Rat
Applications
RNAi
Purity
> 97%
Pricing
$395/15 nmol | $555/30 nmol | $2,440/5x30 nmol
RAD9B, siRNA (Cat# AAA8216247)
Full Name
RAD9B siRNA (Rat)
Reactivity
Rat
Applications
RNAi
Purity
> 97%
Pricing
$395/15 nmol | $555/30 nmol | $2,440/5x30 nmol
Western Blot (WB)
(Western blot analysis of SMC1 expression in (1) HeLa cell lysate; (2) Jurkat cell lysate (MBS179802).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMC1A )
SMC1, Monoclonal Antibody (Cat# AAA179802)
Full Name
Anti-SMC1 Rabbit Monoclonal Antibody
Gene Names
SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Cell cycle control protein 50C (Tmem30c), Recombinant Protein (Cat# AAA7098261)
Full Name
Recombinant Mouse Cell cycle control protein 50C (Tmem30c), partial
Gene Names
Tmem30c; Cdc50c; 4933401B01Rik; 4933409A18Rik
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Cell cycle protein GpsB (gpsB), Recombinant Protein (Cat# AAA1374308)
Full Name
Recombinant Bacillus halodurans Cell cycle protein GpsB (gpsB)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,220/1 mg (E-Coli) | $1,775/1 mg (Yeast) | $2,820/1 mg (Baculovirus) | $1,415/0.1 mg (Baculovirus) | $2,040/0.1 mg (Mammalian-Cell) | $690/0.1 mg (E-Coli) | $900/0.1 mg (Yeast) | $5,685/0.5 mg (Mammalian-Cell) | $1,015/0.02 mg (Baculovirus) | $1,255/0.02 mg (Mammalian-Cell) | $590/0.02 mg (E-Coli) | $770/0.02 mg (Yeast)
Rhodanese-like domain-containing protein 4A, chloroplastic (STR4A), Recombinant Protein (Cat# AAA7082877)
Full Name
Recombinant Arabidopsis thaliana Rhodanese-like domain-containing protein 4A, chloroplastic (STR4A) , partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Western Blot (WB)
CHK1, Monoclonal Antibody (Cat# AAA418378)
Full Name
Anti-CHK1 Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB
Purity
Purified by protein G affinity chromatography
Pricing
$625/0.1 mg | $2,600/5x0.1 mg
RAD9 (S328), Blocking Peptide (Cat# AAA9225056)
Full Name
Phospho-RAD9(S328) Antibody Blocking peptide
Gene Names
RAD9A; RAD9
Proliferation-associated protein 2G4, Monoclonal Antibody (Cat# AAA146690)
Full Name
Mouse Anti Human Proliferation-associated protein 2G4
Gene Names
PA2G4; EBP1; HG4-1; p38-2G4
Reactivity
Human
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
Antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Pricing
$250/0.02 mg | $560/0.1 mg | $180/0.005 mg | $2,300/5x0.1 mg
Knockout Validation
(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: ASNS knockout K562 cell lysate; Predicted MW: 64,62,54kd Observed MW: 62kd Primary Ab: 3ug/ml Rabbit Anti-Human ASNS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Asparagine Synthetase (ASNS), Polyclonal Antibody (Cat# AAA2072791)
Full Name
FITC-Linked Polyclonal Antibody to Asparagine Synthetase (ASNS)
Gene Names
ASNS; TS11; ASNSD
Reactivity
Human
Applications
WB, ICC, IHC, ELISA
Pricing
$205/0.1 mL | $255/0.2 mL | $470/0.5 mL | $575/1 mL | $1,615/5x1 mL
Western Blot (WB)
(Western Blot analysis of various cells with Chk1 Polyclonal Antibody.)
Chk1, Polyclonal Antibody (Cat# AAA2537758)
Full Name
Chk1 Polyclonal Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB, IHC, IF, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)
Asparagine Synthetase (ASNS), ELISA Kit (Cat# AAA2705226)
Full Name
Human Asparagine Synthetase (ASNS) ELISA Kit
Gene Names
ASNS; TS11; ASNSD
Reactivity
Human
Pricing
$320/48-Strip-Wells | $410/96-Strip-Wells | $1,620/5x96-Strip-Wells | $3,120/10x96-Strip-Wells | $245/24-Strip-Wells
PLK1, Blocking Peptide (Cat# AAA9227394)
Full Name
PLK1 Antibody (C-term) Blocking Peptide
Gene Names
PLK1; PLK; STPK13
CDCA3, siRNA (Cat# AAA8238801)
Full Name
CDCA3 siRNA (Rat)
Reactivity
Rat
Applications
RNAi
Purity
> 97%
Pricing
$395/15 nmol | $555/30 nmol | $2,440/5x30 nmol
RCC1, siRNA (Cat# AAA8221288)
Full Name
RCC1 siRNA (Human)
Gene Names
RCC1; CHC1; RCC1-I; SNHG3-RCC1
Reactivity
Human
Applications
RNAi
Purity
> 97%
Pricing
$395/15 nmol | $555/30 nmol | $2,440/5x30 nmol
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PA2G4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate Lane 3. : A549 cell lysate Lane 4. : 293T cell lysate Lane 5. : A431 cell lysate Lane 6. : Jurkat cell lysate)
PA2G4, Monoclonal Antibody (Cat# AAA200436)
Full Name
PA2G4 antibody
Gene Names
PA2G4; EBP1; HG4-1; p38-2G4
Reactivity
Human
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
By protein-A affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Chk1, Polyclonal Antibody (Cat# AAA8520463)
Full Name
Chk1 (phospho Ser296) Polyclonal Antibody
Gene Names
CHEK1; CHK1
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Kinetochore Protein NUF2 (NUF2), ELISA Kit (Cat# AAA9903703)
Full Name
Horse Kinetochore Protein NUF2 (NUF2) ELISA Kit
Gene Names
NUF2; CDCA1; CT106; NUF2R
Reactivity
Horse
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Checkpoint Homolog (CHEK1), Polyclonal Antibody (Cat# AAA2082796)
Full Name
PE-Linked Polyclonal Antibody to Checkpoint Homolog (CHEK1)
Reactivity
Mouse
Applications
WB, ICC, IHC, ELISA
Pricing
$280/0.1 mL | $385/0.2 mL | $730/0.5 mL | $900/1 mL | $2,505/5x1 mL
Cell cycle protein GpsB (gpsB), Recombinant Protein (Cat# AAA1419112)
Full Name
Recombinant Streptococcus pneumoniae Cell cycle protein GpsB (gpsB)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,235/1 mg (E-Coli) | $1,785/1 mg (Yeast) | $2,835/1 mg (Baculovirus) | $1,425/0.1 mg (Baculovirus) | $2,050/0.1 mg (Mammalian-Cell) | $695/0.1 mg (E-Coli) | $910/0.1 mg (Yeast) | $5,690/0.5 mg (Mammalian-Cell) | $1,020/0.02 mg (Baculovirus) | $1,255/0.02 mg (Mammalian-Cell) | $600/0.02 mg (E-Coli) | $775/0.02 mg (Yeast)
Protein C Receptor, Endothelial (PROCR), Polyclonal Antibody (Cat# AAA2035290)
Full Name
APC-Linked Polyclonal Antibody to Protein C Receptor, Endothelial (PROCR)
Reactivity
Human
Applications
WB, ICC, IHC, ELISA
Pricing
$240/0.1 mL | $310/0.2 mL | $590/0.5 mL | $725/1 mL | $2,040/5x1 mL
ECD, Polyclonal Antibody (Cat# AAA1494730)
Full Name
ECD Antibody, FITC conjugated
Gene Names
ECD; GCR2; HSGT1
Reactivity
Human
Applications
EIA
Purity
>95%, Protein G Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various celllines, using RAD17 antibody.)
RAD17, Antibody (Cat# AAA8516733)
Full Name
RAD17 Antibody
Gene Names
RAD17; CCYC; R24L; RAD24; HRAD17; RAD17SP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
CDC27, Polyclonal Antibody (Cat# AAA7047871)
Full Name
CDC27 Antibody, FITC conjugated
Gene Names
CDC27; APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E
Reactivity
Human
Applications
EIA
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded cerebellum tissues (left) and brain tissues (right) using CDK5 antibody with DAB staining.)
CDK5, Monoclonal Antibody (Cat# AAA5305680)
Full Name
Mouse Monoclonal CDK5 antibody
Gene Names
CDK5; LIS7; PSSALRE
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$465/0.1 mg | $2,075/5x0.1 mg
RAD9 (BH3 Domain Specific), Blocking Peptide (Cat# AAA9219733)
Full Name
RAD9 Antibody (BH3 Domain Specific) Blocking peptide
Gene Names
RAD9A; RAD9
SDS-PAGE
CDC37 / CDC37A, Recombinant Protein (Cat# AAA2546770)
Full Name
Recombinant Mouse CDC37 / CDC37A Protein (His & GST tag)
Gene Names
Cdc37; p50; p50Cdc37
Purity
> 85 % as determined by SDS-PAGE
Pricing
$285/0.02 mg | $675/0.1 mg | $3,030/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of 293T cells with Phospho-Chk1 (Ser301) Polyclonal Antibody at dilution of 1:2000)
Chk1, Polyclonal Antibody (Cat# AAA2536288)
Full Name
Phospho-Chk1 (S301) Polyclonal Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Hela cell lysate using RAD1 antibody.)
RAD1, Polyclonal Antibody (Cat# AAA9412841)
Full Name
RAD1 antibody
Gene Names
RAD1; REC1; HRAD1
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
$300/0.05 mL | $390/0.1 mL | $1,610/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Chk1 expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Chk1, Antibody (Cat# AAA8512127)
Full Name
Chk1 Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB, IHC
Purity
Immunogen affinity purified
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from A2780 cells using cdc25A (Ab-75) antibody (Line 1 and 2) and cdc25A (phospho-Ser75) antibody (Line 3 and 4).)
cdc25A, Antibody (Cat# AAA8510096)
Full Name
cdc25A (Ab-75) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
HIP, Recombinant Protein (Cat# AAA5304483)
Full Name
HIP protein
Purity
HIP protein was purified using a a proprietary chromatographic technique.
Pricing
$510/0.025 mg | $2,275/5x0.025 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human placenta, using CDC37 (Phospho-Ser13) Antibody. The picture on the right is blocked with the phospho peptide.)
CDC37, Polyclonal Antibody (Cat# AAA9502829)
Full Name
CDC37 (Phospho-Ser13) Antibody
Gene Names
CDC37; P50CDC37
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TAF1 antibody.)
TAF1, Polyclonal Antibody (Cat# AAA9414492)
Full Name
TAF1 Antibody
Gene Names
TAF1; OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1; TAFII-250; TAF(II)250
Reactivity
Human, Mouse
Applications
IHC
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cJun antibody (Thr239))
cJun, Polyclonal Antibody (Cat# AAA5302868)
Full Name
cJun antibody (Thr239)
Applications
WB, IHC, IF
Purity
cJun antibody was purified by affinity chromatography
Pricing
$465/0.1 mg | $2,075/5x0.1 mg
TERT (S1125), Blocking Peptide (Cat# AAA9225177)
Full Name
Phospho-TERT(S1125) Antibody Blocking peptide
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
MCRS1, siRNA (Cat# AAA8202843)
Full Name
MCRS1 siRNA (Human)
Gene Names
MCRS1; P78; MCRS2; MSP58; INO80Q; ICP22BP
Reactivity
Human
Applications
RNAi
Purity
> 97%
Pricing
$395/15 nmol | $555/30 nmol | $2,440/5x30 nmol
Western Blot (WB)
(Western blot analysis of TSG101 expression in Jurkat cell lysate (MBS179600).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSG101 )
TSG101, Monoclonal Antibody (Cat# AAA179600)
Full Name
Anti-TSG101 Rabbit Monoclonal Antibody
Gene Names
TSG101; TSG10; VPS23
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of MCF-7 cell lysate, using PTEN antibody (MBS179043).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTEN )
PTEN, Monoclonal Antibody (Cat# AAA179043)
Full Name
Anti-PTEN Rabbit Monoclonal Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using p21 Antibody(MBS179142)CDKN1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDKN1A Antibody (MBS179142)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
p21, Monoclonal Antibody (Cat# AAA179142)
Full Name
Anti-p21 Rabbit Monoclonal Antibody
Gene Names
CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Reactivity
Human
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
