RAD9 (BH3 Domain Specific) Blocking Peptide | RAD9A blocking peptide

RAD9 Antibody (BH3 Domain Specific) Blocking peptide

• Gene Names: RAD9A; RAD9
• Synonyms: RAD9 (BH3 Domain Specific); RAD9 Antibody (BH3 Domain Specific) Blocking peptide; Cell cycle checkpoint control protein RAD9A; hRAD9; DNA repair exonuclease rad9 homolog A; RAD9A; RAD9A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the region of human Rad9 BH3 Domain. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 391

• Cellular Location: Nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RAD9A blocking peptide

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

NCBI and Uniprot Product Information

• NCBI GI #: 4759022
• NCBI GeneID: 5883
• NCBI Accession #: NP_004575
• NCBI GenBank Nucleotide #: NM_004584.2
• UniProt Accession #: Q99638; Q6FI29; Q96C41; B2RCZ8
• Molecular Weight: 42,547 Da
• NCBI Official Full Name: cell cycle checkpoint control protein RAD9A isoform 1
• NCBI Official Synonym Full Names: RAD9 checkpoint clamp component A
• NCBI Official Symbol: RAD9A
• NCBI Official Synonym Symbols: RAD9
• NCBI Protein Information: cell cycle checkpoint control protein RAD9A
• UniProt Protein Name: Cell cycle checkpoint control protein RAD9A
• Protein Family: Cell cycle checkpoint control protein
• UniProt Gene Name: RAD9A
• UniProt Synonym Gene Names: hRAD9
• UniProt Entry Name: RAD9A_HUMAN

NCBI Description

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

RAD9A: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.

Protein type: DNA replication; Cell cycle regulation; Deoxyribonuclease; EC 3.1.11.2; Apoptosis

Chromosomal Location of Human Ortholog: 11q13.1-q13.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: 3'-5' exonuclease activity; enzyme binding; histone deacetylase binding; protein binding; protein kinase binding; SH3 domain binding

Biological Process: DNA damage checkpoint; DNA replication; DNA replication checkpoint; intra-S DNA damage checkpoint; response to DNA damage stimulus