Search results for "Goat F ab 2 Anti Mouse IgG H L "

253 results for "Goat F ab 2 Anti Mouse IgG H L " - showing 250-253

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Immunohistochemistry (IHC) (Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human Tubulin in human malignant melanoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human Tubulin in human prostate with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF) (Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.)

Western Blot (WB) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:10000, 1:20000, 1:50000, 1:100000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: Raw246.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: Rabbit heart tissue lysate Lane B: Rabbit liver tissue lysate Lane C: Rabbit spleen tissue lysate Lane D: Rabbit lung tissue lysate Lane E: Rabbit kidney tissue lysate Lane F: Rabbit brain tissue lysate Lane G: Rabbit muscle tissue lysate Lane H: Rabbit stomach tissue lysate Lane I: Rabbit small intestine tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution Lane A: Rat heart tissue lysate Lane B: Rat liver tissue lysate Lane C: Rat spleen tissue lysate Lane D: Rat lung tissue lysate Lane E: Rat kidney tissue lysate Lane F: Rat muscle tissue lysate Lane G: Rat stomach tissue lysate Lane H: Rat small intestine tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution Lane A: Mouse heart tissue lysate Lane B: Mouse liver tissue lysate Lane C: Mouse spleen tissue lysate Lane D: Mouse lung tissue lysate Lane E: Mouse kidney tissue lysate Lane F: Mouse brain tissue lysate Lane G: Mouse muscle tissue lysate Lane H: Mouse stomach tissue lysate Lane I: Mouse pancreas tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: Jurkat Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: Hela Whole Cell Lysate Lane E: RAW264.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Daudi Whole Cell Lysate Lane C: MOLT-4 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lane E: 293T Whole Cell Lysate Lane F: HelaS3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Beta-Tubulin, Monoclonal Antibody (Cat# AAA8100087)

Full Name

Beta-Tubulin Loading Control Antibody, Mouse MAb

Reactivity

Human

Applications

WB, IHC-P, ICC, IF, IP

Purity

Protein A

Pricing

$225/0.05 mL | $310/0.1 mL | $1,235/5x0.1 mL

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Western Blot (WB) (Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: RAW264.7 Whole Cell Lysate Lane D: Mouse slpeen tissue lysate Lane E: Mouse lung tissue lysate Lane F: Mouse brain tissue lysate Lane G: C6 whole cell lysate Lane H: PC-12 whole cell lysate Lane I: Rat liver tissue lysate Lane J: Rat lung tissue lysate Lane K: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary HRP Conjugated Goat anti-Mouse IgG (H+L),at 1:10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:44 kDa)

Western Blot (WB) (Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:44 kDa)

Immunoprecipitation (IP) (ACTB was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 0.5 uL anti-ACTB mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-ACTB mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 42 kDa)

Testing Data (Anti-beta-Actin mouse monoclonal antibody at 1:10000, 1:50000, 1:100000, 1:200000 dilution Lysates/proteins per lane HepG2 (30 ug), Hela (5 ug), Raw246.7 (30 ug) Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size: 44 kDa)

beta-Actin, Monoclonal Antibody (Cat# AAA8100153)

Full Name

beta-Actin Loading Control Antibody, Mouse MAb

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human

Applications

WB, IP

Purity

Protein A

Pricing

$225/0.05 mL | $310/0.1 mL | $1,235/5x0.1 mL

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Immunohistochemistry (IHC) (Immunochemical staining of human Nestin in human brain with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human Nestin in human neurilemmoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human Nestin in human fasciculated sarcoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of Human NES expression on SHSY5Y cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human NES, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Western Blot (WB) (Anti-NES mouse monoclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: U251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:177 kDa)

Immunoprecipitation (IP) (NES was immunoprecipitated using: Lane A:0.5 mg U251MG Whole Cell Lysate 4 uL anti-NES mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NES mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 177 kDa Observed band size :177 kDa)

Nestin, Monoclonal Antibody (Cat# AAA8102271)

Full Name

Anti-Nestin Antibody, Mouse Monoclonal

Gene Names

NES; Nbla00170

Reactivity

Human

Applications

WB, IHC-P, FC/FACS/FCM, IP

Purity

Protein A

Pricing

$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL

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253 results for "Goat F ab 2 Anti Mouse IgG H L " - showing 250-253

Beta-Tubulin, Monoclonal Antibody (Cat# AAA8100087)

beta-Actin, Monoclonal Antibody (Cat# AAA8100153)

Nestin, Monoclonal Antibody (Cat# AAA8102271)

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Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

253 results for "Goat F ab 2 Anti Mouse IgG H L " - showing 250-253

Beta-Tubulin, Monoclonal Antibody (Cat# AAA8100087)

beta-Actin, Monoclonal Antibody (Cat# AAA8100153)

Nestin, Monoclonal Antibody (Cat# AAA8102271)

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