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6202 results for " All Proteins" - showing 2650-2700


Western Blot (WB) (Western blot All lanes: C-C motif chemokine 5 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 10 kDa Observed band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

C-C motif chemokine 5, Polyclonal Antibody (Cat# AAA719945)

Full Name
Rabbit anti-human C-C motif chemokine 5 polyclonal Antibody, HRP conjugated
Gene Names
CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg

IgG, IgA, IgM, H&L, X-adsorbed, Polyclonal Antibody (Cat# AAA618429)

Full Name
IgG, IgA, IgM, H&L, X-adsorbed (Biotin)
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$640/1 mg | $2,865/5x1 mg

Keratin Type II, Monoclonal Antibody (Cat# AAA831123)

Full Name
Keratin Type II antibody
Applications
IHC, WB
Purity
Keratin Type II antibody was purified by Ion-exchange chromatography
Pricing
$655/0.2 mg | $2,930/5x0.2 mg

IgG (H&L), F(ab)'2 Fragment, Secondary Antibody (Cat# AAA687710)

Full Name
Goat anti-Rat IgG (H&L) - Affinity Pure, F(ab)'2 Fragment, Biotin Conjugate, min x w/human, mouse lgG
Reactivity
Rat.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rat serum proteins
IgG from human or mouse
Applications
EIA, IHC
Purity
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Affinity purified using solid phase Rat IgG.
Pricing
$290/0.5 mg | $1,150/5x0.5 mg
Testing Data (Anti-MUC4 Picoband antibody, MBS177464-1.jpgAll lanes: Anti MUC4 (MBS177464) at 0.5ug/mlWB: PC12 Whole Cell Lysate at 40ugPredicted bind size: 234KDObserved bind size: 234KD )

Mucin-4, Polyclonal Antibody (Cat# AAA177464)

Full Name
Anti-MUC4 Antibody
Gene Names
MUC4; ASGP; MUC-4; HSA276359
Reactivity
Human, Rat. No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg

IgG (H&L), F(ab)'2 Fragment, Secondary Antibody (Cat# AAA687812)

Full Name
Goat anti-Rabbit IgG (H&L) - F(ab)'2 Fragment, DyLight550 Conjugate, min x w/bovine, human, or mouse lgG or serum proteins
Reactivity
Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
serum proteins from bovine, human, or mouse
IgG from human or mouse
Applications
FC/FACS, IF, EIA, IM
Purity
> 90% based on SDS-PAGE, Small amounts of intact IgG may be present.
Affinity purified using solid phase Rabbit IgG.
Pricing
$245/0.5 mg | $955/5x0.5 mg
Western Blot (WB) (All lanes : Anti-FBXO32 Antibody (N-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

FBXO32, Polyclonal Antibody (Cat# AAA9216905)

Full Name
FBXO32 Antibody (N-Term)
Gene Names
FBXO32; Fbx32; MAFbx
Reactivity
Human
Applications
WB
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL

IgG Fc, Secondary Antibody (Cat# AAA687648)

Full Name
Goat anti-Rabbit IgG Fc - Affinity Pure, FITC Conjugate
Reactivity
Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
light chains on all rabbit immunoglobulins
Rabbit IgG F(ab)'2 fragment
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rabbit IgG.
Pricing
$210/2 mg | $800/5x2 mg

IgM (u chain), F(ab)'2 Fragment, Secondary Antibody (Cat# AAA687329)

Full Name
Goat anti-Human IgM (u chain), F(ab)'2 Fragment - Affinity Pure
Reactivity
Human.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin human serum proteins
light chains on all human immunoglobulins
Purity
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Affinity purified using solid phase Human IgM.
Pricing
$240/1 mg | $920/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA687702)

Full Name
Goat anti-Rat IgG (H&L) - Affinity Pure, ALP Conjugate, min x w/ human or mouse lgG (highly absorbed against mouse IgG)
Reactivity
Rat.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rat serum proteins
IgG from human or mouse
highly cross absorbed against mouse IgG
Applications
WB, EIA, IHC
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rat IgG.
Pricing
$315/1 mg | $1,260/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA688088)

Full Name
Donkey anti-Sheep IgG (H&L) - Affinity Pure, DyLight594 Conjugate
Reactivity
Sheep.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin sheep serum proteins
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Sheep IgG.
Pricing
$200/1 mg | $740/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA687836)

Full Name
Goat anti-Human IgG (H&L) - Affinity Pure, DyLight800 Conjugate, min x w/bovine, mouse, rabbit serum
Reactivity
Human.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin human serum immunoglobulins
serum proteins from bovine, mouse or rabbit
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Human IgG.
Pricing
$200/1 mg | $740/5x1 mg
Western Blot (WB) (All lanes : Anti-GJB3 Antibody (C211) at 1:1000 dilutionLane 1: Hela whole cell lysateLane 2: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Connexin 31, Polyclonal Antibody (Cat# AAA9217089)

Full Name
Connexin 31 Antibody (C-term)
Gene Names
GJB3; EKV; CX31; DFNA2; DFNA2B
Reactivity
Human
Applications
WB, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed A549, using HLA-DRB3 antibody at 1:500 dilution.)

HLA DRB3, Polyclonal Antibody (Cat# AAA837538)

Full Name
HLA DRB3 antibody
Gene Names
HLA-DRB3; SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B; HLA-DR3B
Applications
IF, ICC, WB
Purity
HLA DRB3 antibody was purified by antigen-affinity chromatography
Pricing
$700/0.1 mL | $3,130/5x0.1 mL
Western Blot (WB) (Anti- SAP97 Picoband antibody, MBS178053, Western blottingAll lanes: Anti SAP97 (MBS178053) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Mouse Lung Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: MM231 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: NIH3T3 Whole Cell Lysate at 40ugPredicted bind size: 130KDObserved bind size: 130KD )

SAP97, Polyclonal Antibody (Cat# AAA178053)

Full Name
Anti-SAP97 Antibody
Gene Names
DLG1; hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg

IgM (u chain), Secondary Antibody (Cat# AAA687324)

Full Name
Goat anti-Human IgM (u chain) - Affinity Pure, Biotin Conjugate, min x w/human IgA + IgG
Reactivity
Human.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin human serum proteins
light chains on all human immunoglobulins
human IgA or IgG
Applications
EIA, IHC
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Human IgM.
Pricing
$185/1 mg | $675/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA687676)

Full Name
Donkey anti-Rat IgG (H&L) - Affinity Pure, FITC Conjugate
Reactivity
Rat.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin rat serum proteins
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rat IgG.
Pricing
$240/2 mg | $920/5x2 mg

Goat anti Rabbit IgG (H + L), Secondary Antibody (Cat# AAA539815)

Full Name
Goat anti Rabbit IgG (H + L)
Reactivity
Rabbit
Applications
User optimized
Purity
Affinity purified using solid phase Rabbit IgG (H&L)
Pricing
$210/2 mg | $940/5x2 mg

IgG (H&L), Secondary Antibody (Cat# AAA688600)

Full Name
Donkey anti-Sheep IgG (H&L) - Affinity Pure, DyLight633 Conjugate, min x w/human,mouse, or rabbit IgG
Reactivity
Sheep.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin sheep serum proteins
IgG from human or rabbit
serum proteins from human or rabbit
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Sheep IgG.
Pricing
$295/1 mg | $1,185/5x1 mg
Testing Data (All lanes: Anti Met (c-Met) (MBS177482) at 0.5ug/mlWB: Rat Liver Tissue Lysate at 50ugPredicted bind size: 154KDObserved bind size: 154KD )

Hepatocyte growth factor receptor, Polyclonal Antibody (Cat# AAA177482)

Full Name
Anti-Met(c-Met) Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met
Reactivity
Human, Rat. No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg

IgG (H&L), F(ab)'2 Fragment, Secondary Antibody (Cat# AAA688322)

Full Name
Donkey anti-Rabbit IgG (H&L) - F(ab)'2 Fragment, DyLight488 Conjugate
Reactivity
Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins
Applications
FC/FACS, IF, EIA, IM
Purity
> 90% based on SDS-PAGE, Small amounts of intact IgG may be present.
Affinity purified using solid phase Rabbit IgG.
Pricing
$245/0.5 mg | $955/5x0.5 mg
Western Blot (WB) (Western Blot analysis in CEM cell line lysates (35ug/lane) using MBS6003686. FCGR1B (arrow) was detected using MBS6003686.)

FCGR1B, Polyclonal Antibody (Cat# AAA6003686)

Full Name
FCGR1B, CT (FCGR1B, IGFRB, High affinity immunoglobulin gamma Fc receptor IB, Fc-gamma RIB)
Gene Names
FCGR1B; CD64b; IGFRB
Reactivity
Human
Applications
EL/EIA, WB, IHC, FC/FACS
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
Western Blot (WB) (Western blot All lanes: Endoplasmic reticulum resident protein 29 antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 29 kDaObserved band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Endoplasmic reticulum resident protein 29, Polyclonal Antibody (Cat# AAA715854)

Full Name
Rabbit anti-human Endoplasmic reticulum resident protein 29 polyclonal Antibody, FITC
Gene Names
ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg

IgM (u chain), Secondary Antibody (Cat# AAA687315)

Full Name
Goat anti-Human IgM (u chain) - Affinity Pure
Reactivity
Human.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin human serum proteins
light chains on all human immunoglobulins
Purity
> 95% based on SDS-PAGE
Affinity purified using solid phase Human IgM.
Pricing
$185/2 mg | $675/5x2 mg

IgG (H&L), Secondary Antibody (Cat# AAA687745)

Full Name
Donkey anti-Sheep IgG (H&L) - Affinity Pure, Biotin Conjugate, min x w/human, mouse, or rabbit IgG
Reactivity
Sheep.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin sheep serum proteins
IgG from human or rabbit
serum proteins from human or rabbit
Applications
EIA, IHC
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Sheep IgG.
Pricing
$250/1 mg | $975/5x1 mg
Western Blot (WB) (All lanes : Anti-MGMT Antibody at1:1000-1:2000 dilutionLane 1: Jurkat whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: MOLT-4 whole cell lysateLane 4: Raji whole cell lysateLysates/proteins at 20 mug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

MGMT, Monoclonal Antibody (Cat# AAA9215442)

Full Name
MGMT Antibody
Reactivity
Human
Applications
WB
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB) (All lanes : Anti-MC3R Antibody (C-term) at 1:2000 dilutionLane 1: mouse large intestine lysatesLane 2: human placenta lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

MC3R, Polyclonal Antibody (Cat# AAA9205690)

Full Name
MC3R Antibody (C-term)
Gene Names
MC3R; MC3; OB20; OQTL; BMIQ9; MC3-R
Reactivity
Human, mouse
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$185/0.08 mL

IgG (H&L), Secondary Antibody (Cat# AAA687559)

Full Name
Rabbit anti-Mouse IgG (H&L) - Affinity Pure, HRP Conjugate, min x w/human serum proteins
Reactivity
Mouse.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin mouse serum proteins
human serum proteins
Applications
WB, EIA, IHC
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Mouse IgG.
Pricing
$200/1 mg | $755/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA687700)

Full Name
Goat anti-Rat IgG (H&L) - Affinity Pure, DyLight488 Conjugate, min x w/human, mouse lgG
Reactivity
Rat.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rat serum proteins
IgG from human or mouse
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rat IgG.
Pricing
$200/1 mg | $750/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA688041)

Full Name
Chicken anti-Rabbit IgG (H&L) - Affinity Pure, DyLight550 Conjugate
Reactivity
Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rabbit IgG.
Pricing
$310/1 mg | $1,240/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA688079)

Full Name
Goat anti-Rabbit IgG (H&L) - Affinity Pure, DyLight650 Conjugate, min x w/bovine, goat, human, mouse, rat IgG or serum proteins
Reactivity
Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
serum proteins from bovine, goat, human, mouse, or rat
IgG from bovine, goat, human, mouse or rat
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rabbit IgG.
Pricing
$280/1 mg | $1,120/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA688331)

Full Name
Goat anti-Mouse IgG (H&L) - Affinity Pure, DyLight594 Conjugate, min x w/bovine, horse, human, pig or rabbit serum proteins
Reactivity
Mouse.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin mouse serum immunoglobulins
serum proteins from bovine, horse, human rabbit or swine
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Mouse IgG.
Pricing
$185/1 mg | $680/5x1 mg
Western Blot (WB) (All lanes : Anti-TERA Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: A549 whole cell lysateLane 4: C6 whole cell lysateLane 5: U-87 MG whole cell lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

TERA, Monoclonal Antibody (Cat# AAA9215476)

Full Name
TERA Antibody
Gene Names
VCP; p97; TERA; ALS14; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB) (Western blot All lanes: Metalloproteinase inhibitor 2 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 24kDa Observed band size: 60kDa Additional bands at: 118 kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Metalloproteinase inhibitor 2, Polyclonal Antibody (Cat# AAA715610)

Full Name
Rabbit anti-human Metalloproteinase inhibitor 2 polyclonal Antibody, FITC
Gene Names
TIMP2; DDC8; CSC-21K
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot All lanes: Glutathione S-transferase P antibody at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 23 kDa Observed band size: 30 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Glutathione S-transferase P, Polyclonal Antibody (Cat# AAA719806)

Full Name
Rabbit anti-human Glutathione S-transferase P polyclonal Antibody, Biotin conjugated
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot All lanes: Prefoldin subunit 5 antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 17 kDaObserved band size: 70 kDa Additional bands at: 80kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Prefoldin subunit 5, Polyclonal Antibody (Cat# AAA715196)

Full Name
Rabbit anti-human Prefoldin subunit 5 polyclonal Antibody, FITC
Gene Names
PFDN5; MM1; MM-1; PFD5
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Flow Cytometry (FC/FACS) (Staining of C57BL/6 splenocytes with NK1.1 antibody and 0.125 ug of CD244.2 antibody (PE). Cells in the lymphocyte gate were used for analysis.)

CD244.2, Monoclonal Antibody (Cat# AAA833816)

Full Name
CD244.2 antibody (PE)
Applications
FC/FACS
Pricing
$545/0.1 mg | $2,450/5x0.1 mg
Western Blot (WB) (All lanes : Anti-ATP5E Antibody (C-Term) at 1:2000 dilutionLane 1: human heart lysateLane 2: human skeletal muscle lysateLane 3: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 6 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

ATP5E, Polyclonal Antibody (Cat# AAA9216954)

Full Name
ATP5E Antibody (C-Term)
Gene Names
ATP5E; ATPE; MC5DN3
Reactivity
Human
Applications
WB
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB) (All lanes : Anti-CERS4 Antibody (N-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: HT-1080 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: LNCaP whole cell lysateLane 5: PC-3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

CERS4, Polyclonal Antibody (Cat# AAA9216892)

Full Name
CERS4 Antibody (N-Term)
Gene Names
CERS4; Trh1; LASS4
Reactivity
Human
Applications
WB
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL

IgG (H&L), Secondary Antibody (Cat# AAA687701)

Full Name
Goat anti-Rat IgG (H&L) - Affinity Pure, min x w/ human or mouse lgG (highly absorbed against mouse IgG)
Reactivity
Rat.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rat serum proteins
IgG from human or mouse
highly cross absorbed against mouse IgG
Purity
> 95% based on SDS-PAGE
Affinity purified using solid phase Rat IgG.
Pricing
$215/2 mg | $815/5x2 mg

IgG (H&L), Secondary Antibody (Cat# AAA687733)

Full Name
Donkey anti-Sheep IgG (H&L) - Affinity Pure, HRP Conjugate
Reactivity
Sheep.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin sheep serum proteins
Applications
WB, EIA, IHC
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Sheep IgG.
Pricing
$195/1 mg | $730/5x1 mg

IgM (u chain), Secondary Antibody (Cat# AAA688512)

Full Name
Goat anti-Human IgM (u chain) - Affinity Pure, DyLight594 Conjugate
Reactivity
Human.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin human serum proteins
light chains on all human immunoglobulins
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Human IgM.
Pricing
$185/1 mg | $685/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA687950)

Full Name
Donkey anti-Sheep IgG (H&L) - Affinity Pure, DyLight550 Conjugate, min x w/human or rabbit IgG
Reactivity
Sheep.
Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human or rabbit serum proteins from human or rabbit
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Sheep IgG.
Pricing
$265/1 mg | $1,040/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA688330)

Full Name
Donkey anti-Rat IgG (H&L) - Affinity Pure, DyLight650 Conjugate
Reactivity
Rat.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin rat serum proteins
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rat IgG.
Pricing
$200/1 mg | $740/5x1 mg

IgG (H&L), Secondary Antibody (Cat# AAA688549)

Full Name
Donkey anti-Rat IgG (H&L) - Affinity Pure, DyLight550 Conjugate
Reactivity
Rat.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin rat serum proteins
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rat IgG.
Pricing
$200/1 mg | $740/5x1 mg
Western Blot (WB) (MDM4 antibody (MBS834160) used at 0.2-1 ug/ml to detect target protein.)

MDM4, Polyclonal Antibody (Cat# AAA834160)

Full Name
MDM4 antibody
Gene Names
MDM4; HDMX; MDMX; MRP1
Applications
WB
Pricing
$535/0.1 mL | $2,395/5x0.1 mL
Western Blot (WB) (Western Blot analysis in HT-29 whole cell lysate (20ug/lane) using MBS645347 (1:2000). Secondary: IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size: 22kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

KRAS, Polyclonal Antibody (Cat# AAA645347)

Full Name
KRAS, CT (KRAS, KRAS2, RASK2, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed)
Reactivity
Human, Mouse
Applications
EL/EIA, WB, FC/FACS, IF
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
Western Blot (WB) (Western blot All lanes: Endoplasmic reticulum resident protein 29 antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 29 kDaObserved band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)

Endoplasmic reticulum resident protein 29, Polyclonal Antibody (Cat# AAA716002)

Full Name
Rabbit anti-human Endoplasmic reticulum resident protein 29 polyclonal Antibody, Biotin conjugated
Gene Names
ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)

EWSR1, Monoclonal Antibody (Cat# AAA415276)

Full Name
EWSR1 Antibody
Gene Names
EWSR1; EWS; bK984G1.4
Applications
IF, WB
Purity
Affinity purified
Pricing
$400/0.1 mL | $1,575/5x0.1 mL
Western Blot (WB) (All lanes : Anti-FOXE3 Antibody at 1:1000 dilutionLane 1: HepG2 whole cell lysateLane 2: A549 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

FOXE3, Polyclonal Antibody (Cat# AAA9216251)

Full Name
FOXE3 Antibody
Gene Names
FOXE3; FKHL12; FREAC8
Reactivity
Human, Mouse
Applications
WB
Pricing
$405/0.1 mL | $1,585/5x0.1 mL
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