$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB) (GFAP rabbit polyclonal antibody. Western Blot analysis of GFAP expression in mouse intestine.)
Western Blot (WB) (Western Blot analysis of GFAP expression in transfected 293T cell line by GFAP polyclonal antibody. Lane 1: GFAP transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)
Testing Data (Staining of a Kinesin, Heavy Chain in a PtK1 cell (red). The cell is double labeled with antiserum to tubulin (green). The kinesin positive structures correspond to the intermediate compartment, a membrane system. EAAC1-immunoreactivity is present in granules of pyramidal neuron and nerve fibers. Note that EAAC1-immunoreactivity is exclusively neuronal and distinct from GFAP-positive glial elements.)
Western Blot (WB) (NRG1 rabbit polyclonal antibody. Western Blot analysis of NRG1 expression in human placenta.)
Western Blot (WB) (Western Blot analysis of NRG1 expression in transfected 293T cell line by NRG1 polyclonal antibody. Lane 1: NRG1 transfected lysate (26.1kD). Lane 2: Non-transfected lysate.)
Testing Data (Detection limit for recombinant GST tagged GCM1 is approximately 1ng/ml as a capture antibody.)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to GCM1 on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to GCM1 on HeLa cell. [antibody concentration 10 ug/ml])
Western Blot (WB) (Western Blot analysis of GDNF expression in transfected 293T cell line by GDNF polyclonal antibody. Lane 1: GDNF transfected lysate (23.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB) (GCM1 monoclonal antibody Western Blot analysis of GCM1 expression in FHs 173 WE.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to GCM1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.7ug/ml])
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to GCM1 on HeLa cell. [antibody concentration 10ug/ml].)
Western Blot (WB) (GCM1 monoclonal antibody Western Blot analysis of GCM1 expression in FHs 173 WE.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to GCM1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.7ug/ml])
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to GCM1 on HeLa cell. [antibody concentration 10ug/ml].)
Western Blot (WB) (NKX6-2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NKX6-2 antibody detected the NKX6-2 protein (arrow).)
Immunohistochemistry (IHC) (Nestin staining of mouse pancreas usingMBS600695. Tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25ug/ml, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.)
Immunohistochemistry (IHC) (Negative control staining of mouse pancreas usingMBS600695. Tissue pretreated with citrate buffer, pH 6.0. Negative Control Reagent, using IHC-Select Detection with HRP-DAB. No signal detected.)
Testing Data (Immunolocalization of Nestin in mouse brain sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin. The blue is Nissl stain (rough ER), the red is nestin MBS600695, and the green is either lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes).)
Immunohistochemistry (IHC) (Nestin staining of mouse pancreas usingMBS600695. Tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25ug/ml, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.)
Immunohistochemistry (IHC) (Negative control staining of mouse pancreas usingMBS600695. Tissue pretreated with citrate buffer, pH 6.0. Negative Control Reagent, using IHC-Select Detection with HRP-DAB. No signal detected.)
Testing Data (Immunolocalization of Nestin in mouse brain sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin. The blue is Nissl stain (rough ER), the red is nestin MBS600695, and the green is either lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes).)
Immunohistochemistry (IHC)-Paraffin (The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using MBS7129065(NRG1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))
SDS-Page (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse brain tissue, A172 cells, Primary antibody: MBS7129065(NRG1 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Western Blot (WB) (Western blot analysis of GDNF Antibody (N-term) in mouse NIH-3T3 cell line lysates (35ug/lane). GDNF (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (GDNF Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western Blot Analysis: Rat brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with C5067-70D (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.)
Testing Data (Rat Brain (Lane 1) and Mouse Brain Membrane were resolved by electrophoresis, transferred to PVDF membrane and probed with C5067-70D (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.)
Testing Data (C5067-70D staining of adult rat hippocampus. Photo shows single oligodendrocyte precursor cell.)
Testing Data (C5067-70D staining of adult rat cortex. Photo shows oligodendrocyte precursor cell.)