Rabbit anti-Human HLA-DQA1 Polyclonal Antibody | anti-HLA-DQA1 antibody

HLA-DQA1, NT (HLA-DQA1, HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1) (FITC)

Cat. Num. AAA6307113

• Gene Names: HLA-DQA1; DQA1; DQ-A1; CELIAC1; HLA-DQA
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HLA-DQA1; Polyclonal Antibody; NT (HLA-DQA1; HLA class II histocompatibility antigen; DQ alpha 1 chain; DC-1 alpha chain; DC-alpha; HLA-DCA; MHC class II DQA1) (FITC); MHC class II DQA1; anti-HLA-DQA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HLA-DQA1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HLA-DQA1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 39-66 in the N-terminal region of human HLA-DQA1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS646148. This demonstrates that MBS646148 detected the HLA-DQA1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) using MBS646148 Lane 1: nontransfected Lane 2: transiently transfected with the HLA-DQA1 gene.)

Western Blot (WB)

(Western Blot analysis in Raji whole cell lysate (20ug/lane) using MBS646148 (1:1000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 28kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using MBS646148 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HLA-DQA1 antibody

Antibodies; HLA

References

1. Donaldson, P.T., et al. J. Hepatol. 53(6):1049-1053(2010). 2. Noble, J.A., et al. Diabetes 59(11):2972-2979(2010). 3. Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010). 4. Ovsyannikova, I.G., et al. PLoS ONE 5 (7), E11806 (2010). 5. Xun, Y.H., et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 23(6):430-433(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 169217612
• NCBI GeneID: 3117
• UniProt Accession #: P01909
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DQ alpha 1
• NCBI Official Symbol: HLA-DQA1
• NCBI Official Synonym Symbols: DQA1; DQ-A1; CELIAC1; HLA-DQA
• NCBI Protein Information: HLA class II histocompatibility antigen, DQ alpha 1 chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DQ alpha 1 chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DQA1
• UniProt Entry Name: DQA1_HUMAN

NCBI Description

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DQA1 iso4:

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; membrane; lysosomal membrane; integral to plasma membrane; plasma membrane; trans-Golgi network membrane; endosome membrane; MHC class II protein complex; external side of plasma membrane

Molecular Function: MHC class II receptor activity; peptide antigen binding

Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; T cell receptor signaling pathway