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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for " Protein Kinase c" - showing 1400-1450
Immunofluorescence (IF)
(MBS9609056 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
ICK, Polyclonal Antibody (Cat# AAA9609056)
Full Name
Phospho-ICK (Tyr159) Antibody
Gene Names
ICK; ECO; MRK; LCK2; EJM10
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Anti-RAF1 Antibody at 1:1000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
RAF1, Polyclonal Antibody (Cat# AAA9217154)
Full Name
RAF1 Antibody (S621)
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human; Predicted Reactivity: Bovine, Chicken, Mouse, Rat
Applications
WB, IHC, EIA
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of PKC delta phosphorylation expression in MCF7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
PKC delta, Polyclonal Antibody (Cat# AAA9601177)
Full Name
Phospho-PKC delta (Ser645) Antibody
Gene Names
PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis on 293 cell lysate using Phospho-AKT1(Thr308) Antibody. The lane on the left is treated with the antigen-specific peptide.)
AKT1, Polyclonal Antibody (Cat# AAA9600582)
Full Name
Phospho-AKT1(Thr308) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9601613 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
hnRNP K, Polyclonal Antibody (Cat# AAA9601613)
Full Name
hnRNP K (Phospho-Ser284) Antibody
Gene Names
HNRNPK; AUKS; CSBP; TUNP; HNRPK
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9601665 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
SGK, Polyclonal Antibody (Cat# AAA9601665)
Full Name
SGK Antibody
Gene Names
SGK1; SGK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9601078 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
mTOR, Polyclonal Antibody (Cat# AAA9601078)
Full Name
Phospho-mTOR (Ser2481) Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of IKK-gamma phosphorylation expression in TNF-a treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IKK gamma, Polyclonal Antibody (Cat# AAA9601264)
Full Name
Phospho-IKK gamma (Ser31) Antibody
Gene Names
IKBKG; IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma
Reactivity
Human
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9601884 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ZAP-70, Polyclonal Antibody (Cat# AAA9601884)
Full Name
ZAP-70 Antibody
Gene Names
ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of ErbB 4 using anti-ErbB 4 antibody (MBS1750368).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human PANC-1 cell lysates,Lane 2: human placenta tissue lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ErbB 4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ErbB 4 at approximately 180KD. The expected band size for ErbB 4 is at 147KD. )
ErbB 4, Polyclonal Antibody (Cat# AAA1750368)
Full Name
Anti-ErbB 4 Picoband antibody
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of MCF7, using Eif2ak2 antibody. The lane on the left is treated with the antigen-specific peptide.)
EIF2AK2, Polyclonal Antibody (Cat# AAA9608048)
Full Name
EIF2AK2 Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9601699 staining A-431 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
EGFR, Polyclonal Antibody (Cat# AAA9601699)
Full Name
EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (TD)
(MBS9203594 staining CDKN1A in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody)
CDKN1A, Polyclonal Antibody (Cat# AAA9203594)
Full Name
CDKN1A Antibody (C-term)
Gene Names
CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Reactivity
Human, mouse
Applications
IHC-P , WB , IF , EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
MSK2, Polyclonal Antibody (Cat# AAA9610519)
Full Name
Phospho-MSK2 (Ser360) Antibody
Gene Names
RPS6KA4; MSK2; RSK-B; S6K-alpha-4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB)
(Western blot analysis of EPS8L1 expression in Hela (A), COLO205 (B), mouse uterus (C), rat placenta (D) whole cell lysates.)
EPS8L1, Polyclonal Antibody (Cat# AAA8248136)
Full Name
Anti-EPS8L1 Antibody
Gene Names
EPS8L1; DRC3; EPS8R1; PP10566
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC
Purity
The antibody was purified by affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Western Blot (WB)
(Western blot analysis of CKIP-1 Antibody (N-term) in 293 cell line lysates (35ug/lane). CKIP-1 (arrow) was detected using the purified Pab.)
CKIP-1, Polyclonal Antibody (Cat# AAA9207531)
Full Name
CKIP-1 Antibody (N-term)
Gene Names
PLEKHO1; JBP; CKIP1; OC120; CKIP-1
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Phospho-Src-S17, Antibody (Cat# AAA9128999)
Full Name
Phospho-Src-S17 Polyclonal Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using MBS768260 (c-SRC Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .)
c-SRC, Monoclonal Antibody (Cat# AAA768260)
Full Name
c-SRC Mouse Monoclonal
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Hamster
Applications
EIA, WB, IHC
Purity
>=95% as determined by SDS-PAGE
Protein A+G purification
Protein A+G purification
Pricing
$365/0.1 mg | $1,530/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-PKCB (Ser661) in lysates of HeLa heat shock, using Phospho-PKCB (Ser661) Antibody.)
PKCB, Polyclonal Antibody (Cat# AAA9610552)
Full Name
Phospho-PKCB (Ser661) Antibody
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Sheep, Rabbit
Predicted: Bovine, Sheep, Rabbit
Applications
WB, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)
Arg, ELISA Kit (Cat# AAA2507239)
Full Name
Mouse Arg (Arginase) ELISA Kit
Gene Names
ABL2; ARG; ABLL
Reactivity
Mouse
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NME2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
NME2, Polyclonal Antibody (Cat# AAA9125304)
Full Name
NME2
Gene Names
NME2; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Eps8L3 using COS7 whole cell lysates)
Eps8L3, Polyclonal Antibody (Cat# AAA9602104)
Full Name
Eps8L3 Antibody
Gene Names
EPS8L3; EPS8R3
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9604867 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CIB3, Polyclonal Antibody (Cat# AAA9604867)
Full Name
CIB3 Antibody
Gene Names
CIB3; KIP3
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9603840 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDK8, Polyclonal Antibody (Cat# AAA9603840)
Full Name
CDK8 Antibody
Gene Names
CDK8; K35
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Erk1/2, Polyclonal Antibody (Cat# AAA805912)
Full Name
Erk1/2 Antibody: Biotin
Gene Names
Mapk3; p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
Reactivity
Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, Xenopus
Applications
WB, IHC, ICC, IF
Pricing
$425/0.1 mL | $1,690/5x0.1 mL
Immunohistochemistry (IHC)
(MBS9607378 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PGK1, Polyclonal Antibody (Cat# AAA9607378)
Full Name
PGK1 Antibody
Gene Names
PGK1; PGKA; MIG10; HEL-S-68p
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis on HepG2 cell lysate using MOK Antibody. The lane on the left is treated with the antigen-specific peptide.)
MOK, Polyclonal Antibody (Cat# AAA9600562)
Full Name
MOK Antibody
Gene Names
MOK; RAGE; RAGE1; STK30; RAGE-1
Reactivity
Human
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of MARCKS expression in Mouse brain tissue lysates, The lane on the left is treated with the antigen-specific peptide.)
MARCKS, Polyclonal Antibody (Cat# AAA9601840)
Full Name
MARCKS Antibody
Gene Names
MARCKS; MACS; 80K-L; PKCSL; PRKCSL
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of MEK2 expression in ovarycancer whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
MEK2, Polyclonal Antibody (Cat# AAA9601859)
Full Name
MEK2 Antibody
Gene Names
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9604674 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
MAPK10, Polyclonal Antibody (Cat# AAA9604674)
Full Name
MAPK10 Antibody
Gene Names
MAPK10; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 1, Large Subunit (CAPN1), Active Protein (Cat# AAA2097302)
Full Name
Active Calpain 1, Large Subunit (CAPN1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$220/0.01 mg | $465/0.05 mg | $710/0.1 mg | $880/0.2 mg | $1,715/0.5 mg | $2,555/1 mg | $6,250/5 mg
Western Blot (WB)
(Western blot analysis of GSK3B expression in various lysates)
GSK3A/B, Polyclonal Antibody (Cat# AAA9607461)
Full Name
GSK3A/B Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis on COLO205 cell lysate using DOK6 Antibody. The lane on the left is treated with the antigen-specific peptide.)
DOK6, Polyclonal Antibody (Cat# AAA9600556)
Full Name
DOK6 Antibody
Gene Names
DOK6; DOK5L; HsT3226
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human Jurkat Cells; Lane3: Human HepG2 Cells; Lane4: Human U2OS Cells; Lane5: Human A549 Cells.)
Protein Kinase D1, Polyclonal Antibody (Cat# AAA2026250)
Full Name
Polyclonal Antibody to Protein Kinase D1 (PKD1)
Gene Names
PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$155/0.1 mL | $190/0.2 mL | $310/0.5 mL | $385/1 mL | $1,120/5x1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.)
CaMKII, Monoclonal Antibody (Cat# AAA804370)
Full Name
CaMKII Antibody
Gene Names
Camk2a; PKCCD; PK2CDD
Reactivity
Rat, Human
Applications
EIA, IF, IP, WB
Pricing
$405/0.1 mg | $1,585/5x0.1 mg
Western Blot (WB)
(Western blot analysis on 293 cell lysate using MOL1A Antibody. The lane on the left is treated with the antigen-specific peptide.)
MOL1A, Polyclonal Antibody (Cat# AAA9600538)
Full Name
MOL1A Antibody
Gene Names
MOB1B; MATS2; MOB4A; MOBKL1A
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9601959 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
PKC delta, Polyclonal Antibody (Cat# AAA9601959)
Full Name
PKC delta Antibody
Gene Names
PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from A549 cells, using CERKL antibody.The lane on the left is treated with the antigen-specific peptide.)
CERKL, Polyclonal Antibody (Cat# AAA9604774)
Full Name
CERKL Antibody
Gene Names
CERKL; RP26
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9604869 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
RPS6KC1, Polyclonal Antibody (Cat# AAA9604869)
Full Name
RPS6KC1 Antibody
Gene Names
RPS6KC1; RSKL1; RPK118; S6PKh1; humS6PKh1; S6K-delta-1
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9200291 MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).)
MET/HGFR, Monoclonal Antibody (Cat# AAA9200291)
Full Name
MET/HGFR Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human, mouse
Applications
IF, EIA, IHC, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of Phospho-SEK1/MKK4 (Ser80) expression in various lysates)
SEK1/MKK4, Polyclonal Antibody (Cat# AAA9601090)
Full Name
Phospho-SEK1/MKK4 (Ser80) Antibody
Gene Names
MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9601730 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Fyn, Polyclonal Antibody (Cat# AAA9601730)
Full Name
Fyn Antibody
Gene Names
FYN; SLK; SYN; p59-FYN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells, using TAOK1 antibody.)
TAOK1, Polyclonal Antibody (Cat# AAA9605930)
Full Name
TAOK1 Antibody
Gene Names
TAOK1; PSK2; TAO1; KFC-B; MARKK; PSK-2; hKFC-B; hTAOK1; MAP3K16
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9607498 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
AURKA, Polyclonal Antibody (Cat# AAA9607498)
Full Name
AURKA Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9607265 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
BUB1B, Polyclonal Antibody (Cat# AAA9607265)
Full Name
BUB1B Antibody
Gene Names
BUB1B; MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from K562 cells using DDR1 antibody.)
DDR1, Polyclonal Antibody (Cat# AAA9606230)
Full Name
DDR1 Antibody
Gene Names
DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
Reactivity
Human, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis using FAK mouse mAb against A549 (1) and NIH/3T3 (2) cell lysate.)
FAK, Antibody (Cat# AAA850912)
Full Name
FAK Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse
Applications
IF, FC
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MAP2K7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
MAP2K7, Polyclonal Antibody (Cat# AAA126816)
Full Name
MAP2K7 Polyclonal Antibody
Gene Names
MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
Reactivity
Human, Mouse
Applications
WB, IHC, ICC, IF, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysates from Hela cell line,mouse liver tissue lysate(from left to right), using TYK2 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
TYK2, Polyclonal Antibody (Cat# AAA9231919)
Full Name
TYK2 Antibody (C-term)
Gene Names
TYK2; JTK1; IMD35
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Aurora A expression in MCF7 (A), SP2/0 (B), H9C2 (C) whole cell lysates.)
Aurora A, Polyclonal Antibody (Cat# AAA8205058)
Full Name
Anti-Aurora A Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47
Reactivity
Human, Mouse, Rat, Porcine, Monkey
Applications
WB, IHC, IF, ICC
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
