Goat PKD1 / Polycystin Polyclonal Antibody | anti-PKD1 antibody

Goat Polyclonal to Human PKD1 / Polycystin

Cat. Num. AAA244275

• Gene Names: PKD1; PBP; Pc-1; TRPP1
• Reactivity: Gibbon, Gorilla, Human
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: PKD1 / Polycystin; Polyclonal Antibody; Goat Polyclonal to Human PKD1 / Polycystin; Anti-PKD1 / Polycystin Antibody IHC-plus; PKD1; PBP; Polycystin; TRPP1; Pc-1; Polycystin 1; Polycystin-1; Human PKD1; anti-PKD1 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human
• Clonality: Polyclonal
• Specificity: Human PKD1. This antibody is expected to recognize both reported isoforms (NP_001009944.1 and NP_000287.2).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 4302

• Applicable Applications for anti-PKD1 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3.75 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RTPLRAKNKVHP from the C-terminus of human PKD1 (NP_001009944.1; NP_000287.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Marmoset, Panda, Cat, Dog, Horse (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: PKD1 / Polycystin antibody was raised against synthetic peptide C-RTPLRAKNKVHP from the C-terminus of human PKD1 (NP_001009944.1; NP_000287.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Marmoset, Panda, Cat, Dog, Horse (83%).
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PKD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PKD1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 205360962
• NCBI GeneID: 5310
• NCBI Accession #: NP_000287.3
• NCBI GenBank Nucleotide #: NM_000296.3
• UniProt Accession #: P98161; Q15140; Q15141
• Molecular Weight: 462,401 Da
• NCBI Official Full Name: polycystin-1 isoform 2
• NCBI Official Synonym Full Names: polycystic kidney disease 1 (autosomal dominant)
• NCBI Official Symbol: PKD1
• NCBI Official Synonym Symbols: PBP; Pc-1; TRPP1
• NCBI Protein Information: polycystin-1; polycystin 1; polycystic kidney disease-associated protein; autosomal dominant polycystic kidney disease 1 protein; transient receptor potential cation channel, subfamily P, member 1
• UniProt Protein Name: Polycystin-1
• Protein Family: Polycystin
• UniProt Gene Name: PKD1
• UniProt Entry Name: PKD1_HUMAN

NCBI Description

This gene encodes a member of the polycystin protein family. The encoded glycoprotein contains a large N-terminal extracellular region, multiple transmembrane domains and a cytoplasmic C-tail. It is an integral membrane protein that functions as a regulator of calcium permeable cation channels and intracellular calcium homoeostasis. It is also involved in cell-cell/matrix interactions and may modulate G-protein-coupled signal-transduction pathways. It plays a role in renal tubular development, and mutations in this gene cause autosomal dominant polycystic kidney disease type 1 (ADPKD1). ADPKD1 is characterized by the growth of fluid-filled cysts that replace normal renal tissue and result in end-stage renal failure. Splice variants encoding different isoforms have been noted for this gene. Also, six pseudogenes, closely linked in a known duplicated region on chromosome 16p, have been described. [provided by RefSeq, Oct 2008]

Uniprot Description

PKD1: Involved in renal tubulogenesis. Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. Acts as a regulator of cilium length, together with PKD2. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. May be an ion- channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions. Defects in PKD1 are the cause of polycystic kidney disease autosomal dominant type 1 (ADPKD1). ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. Its prevalence is estimated at about 1/1000. Belongs to the polycystin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell adhesion

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi membrane; basolateral plasma membrane; integral to plasma membrane; cytoplasm; motile primary cilium; plasma membrane; integral to membrane; nucleus; lateral plasma membrane; cilium

Molecular Function: protein domain specific binding; protein binding; calcium channel activity; carbohydrate binding; protein kinase binding

Biological Process: skin development; neural tube development; embryonic placenta development; positive regulation of protein binding; cell-matrix adhesion; heart development; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; cell-cell adhesion; nitrogen compound metabolic process; elevation of cytosolic calcium ion concentration; protein export from nucleus; cell cycle arrest; kidney development; cartilage condensation; cytoplasmic sequestering of transcription factor; anatomical structure morphogenesis; in utero embryonic development; organelle organization and biogenesis; detection of mechanical stimulus; JAK-STAT cascade; liver development; genitalia development; peptidyl-serine phosphorylation; gut development; branching morphogenesis of a tube; spinal cord development; cartilage development; calcium-independent cell-matrix adhesion; positive regulation of transcription from RNA polymerase II promoter; homophilic cell adhesion

Disease: Polycystic Kidney Disease 1