Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1199 results for "28-kda-antigen" - showing 50-100
Western Blot (WB)
(Figure 1. Western blot analysis of Aquaporin 1 using anti- Aquaporin 1 antibody (MBS175198). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Kidney Tissue Lysate, Lane 2: Rat Lung Tissue Lysate, Lane 3: SMMC Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Aquaporin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Aquaporin 1 at approximately 28KD. The expected band size for Aquaporin 1 is at 28KD. )
Aquaporin 1, Polyclonal Antibody (Cat# AAA175198)
Full Name
Anti-Aquaporin 1 antibody
Gene Names
AQP1; CO; CHIP28; AQP-CHIP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Glutathione S-Transferase, Active Protein (Cat# AAA143838)
Full Name
Recombinant Glutathione S-Transferase His Tag
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
$180/0.01 mg | $250/0.05 mg | $2,380/1 mg | $10,490/5x1 mg
Testing Data
(Formalin-paraffin human melanoma tissue stained with gp100 Ab (NKI-beteb),)
gp100, Monoclonal Antibody (Cat# AAA438452)
Full Name
gp100/ Melanosome/ PMEL17/ SILV
Gene Names
PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
Reactivity
Human, Horse.Does not react with rat, Others-not tested.
Applications
EIA, FC/FACS, Immunofluorescence (IF, IH
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blotAll lanes: TM9SF1 antibody at 6.93ug/ml+U251 whole cell lysateGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 69,56 kDaObserved band size: 69,46 kDa)
TM9SF1, Polyclonal Antibody (Cat# AAA7045317)
Full Name
TM9SF1 Antibody
Gene Names
TM9SF1; MP70; HMP70
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CD81 in human lung tissue lysate with CD81 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking petide.)
CD81, Polyclonal Antibody (Cat# AAA151030)
Full Name
CD81 Antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
CD81 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA214958)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Mouse pancreas tissue using ILK Polyclonal Antibody at dilution of 1:550)
ILK, Polyclonal Antibody (Cat# AAA2525642)
Full Name
ILK Polyclonal Antibody
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9601340 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Cathepsin G, Polyclonal Antibody (Cat# AAA9601340)
Full Name
Cathepsin G Antibody
Gene Names
CTSG; CG; CATG
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blotAll lanes: BCAP29 antibody at 2.93ug/mlLane 1:Raji whole cell lysateLane 2:Colo320 whole cell lysateLane 3:rat gonad tissueLane 4:THP-1 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 29,41 kDaObserved band size: 28 kDa)
BCAP29, Polyclonal Antibody (Cat# AAA7044559)
Full Name
BCAP29 Antibody
Gene Names
BCAP29; BAP29
Reactivity
Human, Rat
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of 22RV1 cells using HSP27 Polyclonal Antibody at dilution of 1:1000.)
HSP27, Polyclonal Antibody (Cat# AAA2535595)
Full Name
HSP27 Polyclonal Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210664)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Western Blot (WB)
(Western blot analysis of VEGF expression in rat spleen tissue sample.)
VEGF, Polyclonal Antibody (Cat# AAA9601304)
Full Name
VEGF Antibody
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from various samples, using CD81 Antibody. Lane 1: Hela treated with blocking peptide Lane 2: HelaLane 3: HepG2.)
CD81, Polyclonal Antibody (Cat# AAA9603837)
Full Name
CD81 Antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Test Data
(All lanes: Anti-CD81 Antibody at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Ramos whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
CD81, Monoclonal Antibody (Cat# AAA9217050)
Full Name
CD81 Antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human
Applications
WB, EIA
Pricing
$190/0.05 mL | $435/0.2 mL | $1,705/5x0.2 mL
Western Blot (WB)
(Western blot analysis of BCL-2 phosphorylation expression in LPS treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
BCL-2, Polyclonal Antibody (Cat# AAA9600912)
Full Name
Phospho-BCL-2 (Ser70) Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of A431 cells, using Phospho-Rac1/cdc42 (Ser71) Antibody.)
Rac1/cdc42, Polyclonal Antibody (Cat# AAA9600721)
Full Name
Phospho-Rac1/cdc42 (Ser71) Antibody
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of MCF-7 cells, using Mast Cell Chymase antibody. The lane of the left was treated with blocking peptide.)
Mast Cell Chymase, Polyclonal Antibody (Cat# AAA9603518)
Full Name
Mast Cell Chymase Antibody
Gene Names
CMA1; CYH; MCT1; chymase
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9601125 at 1/100 staining human Breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
14-3-3 zeta, Polyclonal Antibody (Cat# AAA9601125)
Full Name
Phospho-14-3-3 zeta (Ser58) Antibody
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).)
CD81/TAPA-1, Monoclonal Antibody (Cat# AAA4380374)
Full Name
CD81/TAPA-1
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat. Others not known.
Applications
FC, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (MBS176940).Adiponectin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Adiponectin Antibody (MBS176940) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Adiponectin, Polyclonal Antibody (Cat# AAA176940)
Full Name
Anti-Adiponectin antibody
Gene Names
Adipoq; Acdc; Acrp30
Reactivity
Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(MBS9604837 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
BRI3B, Polyclonal Antibody (Cat# AAA9604837)
Full Name
BRI3B Antibody
Gene Names
BRI3BP; KG19; BNAS1; HCCR-1; HCCR-2; HCCRBP-1; HCCRBP-3
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
Aquaporin 1, Polyclonal Antibody (Cat# AAA540618)
Full Name
Aquaporin 1 Antibody
Gene Names
AQP1; CO; CHIP28; AQP-CHIP
Reactivity
Bovine, Human, Mouse, Rat, Sheep
Applications
EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
SDS-Page
Hsp27, Recombinant Protein (Cat# AAA203034)
Full Name
Hsp27, 1-205aa, Human, E Coli
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$190/0.02 mg | $280/0.1 mg | $570/0.5 mg | $2,325/5x0.5 mg
Immunofluorescence (IF)
(MBS9601925 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
14-3-3 zeta, Polyclonal Antibody (Cat# AAA9601925)
Full Name
14-3-3 zeta Antibody
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from 293, rat brain, mouse lung, rat spleen, using 14-3-3 gamma Antibody.)
14-3-3 gamma, Polyclonal Antibody (Cat# AAA9604950)
Full Name
14-3-3 gamma Antibody
Gene Names
YWHAG; EIEE56; PPP1R170; 14-3-3GAMMA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Mouse lung tissue lysate, using IL27 Antibody. The lane on the left is treated with the antigen-specific peptide.)
IL27, Polyclonal Antibody (Cat# AAA9608014)
Full Name
IL27 Antibody
Gene Names
IL27; p28; IL30; IL-27; IL27A; IL-27A; IL27p28
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9607044 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
BDNF, Polyclonal Antibody (Cat# AAA9607044)
Full Name
BDNF Antibody
Gene Names
BDNF; ANON2; BULN2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of MRPL28 using COS7 whole cell lysates)
MRPL28, Polyclonal Antibody (Cat# AAA9609422)
Full Name
MRPL28 Antibody
Gene Names
MRPL28; p15; MAAT1
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from mouse spleen, using CD28 Antibody.The lane on the right is treated with the antigen-specific peptide.)
CD28, Polyclonal Antibody (Cat# AAA9600003)
Full Name
CD28 Antibody
Gene Names
CD28; Tp44
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA216888)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$1,350/2 mg | $5,915/5x2 mg
Western Blot (WB)
(Western blot analysis on RAW264.7 cell lysate using 14-3-3 beta/zeta Antibody.The lane on the left is treated with the antigen-specific peptide.)
14-3-3 beta/zeta, Polyclonal Antibody (Cat# AAA9600044)
Full Name
14-3-3 beta/zeta Antibody
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Glutathione S-Transferase, Active Protein (Cat# AAA143575)
Full Name
Recombinant Glutathione S-Transferase
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
$180/0.01 mg | $250/0.05 mg | $1,925/1 mg | $8,435/5x1 mg
Western Blot (WB)
(Western blot analysis of MPZ Antibody (C-term) in CEM cell line lysates (35ug/lane). MPZ (arrow) was detected using the purified Pab.)
MPZ, Polyclonal Antibody (Cat# AAA9207336)
Full Name
MPZ Antibody (C-term)
Gene Names
MPZ; P0; CHM; DSS; MPP; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; HMSNIB
Reactivity
Human
Applications
EIA, IHC-p, WB, FC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts from P19 cells(UV treatment), using PRRX2 Ab. The lane on the left was treated with blocking peptide.)
PRRX2, Polyclonal Antibody (Cat# AAA9610046)
Full Name
PRRX2 Antibody
Gene Names
PRRX2; PMX2; PRX2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216587)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
FC/FACS
Pricing
$280/0.05 mg | $1,115/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts from mouse muscle, using CD151 Antibody.)
CD151, Polyclonal Antibody (Cat# AAA9607355)
Full Name
CD151 Antibody
Gene Names
CD151; GP27; MER2; RAPH; SFA1; PETA-3; TSPAN24
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using AQP8 Antibody. Lane 1 was treated with the blocking peptide.)
AQP8, Polyclonal Antibody (Cat# AAA9609541)
Full Name
AQP8 Antibody
Gene Names
AQP8; AQP-8
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Connexin 32 expression in LOVO cells, The lane on the left is treated with the antigen-specific peptide.)
Connexin 32, Polyclonal Antibody (Cat# AAA9601305)
Full Name
Connexin 32 Antibody
Gene Names
GJB1; CMTX; CX32; CMTX1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9602216 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rab 11B, Polyclonal Antibody (Cat# AAA9602216)
Full Name
Rab 11B Antibody
Gene Names
RAB11B; H-YPT3; NDAGSCW
Reactivity
Human, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9600329 at 1/100 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
TALL-2, Polyclonal Antibody (Cat# AAA9600329)
Full Name
TALL-2 Antibody
Gene Names
TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TNLG7B; TRDL-1; UNQ383/PRO715
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9605117 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
MRPL16, Polyclonal Antibody (Cat# AAA9605117)
Full Name
MRPL16 Antibody
Gene Names
MRPL16; L16mt; MRP-L16; PNAS-111
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-PRAS40 (Ser183) Antibody.)
PRAS40, Polyclonal Antibody (Cat# AAA9600714)
Full Name
Phospho-PRAS40 (Ser183) Antibody
Gene Names
AKT1S1; Lobe; PRAS40
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis Granzyme H using K562 whole cell lysates)
Granzyme H, Polyclonal Antibody (Cat# AAA9602121)
Full Name
Granzyme H Antibody
Gene Names
GZMH; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of mouse eyes tissue lysate, using AQP0 antibody. The lane on the left is treated with the antigen-specific peptide.)
AQP0, Polyclonal Antibody (Cat# AAA9607746)
Full Name
AQP0 Antibody
Gene Names
MIP; AQP0; LIM1; MP26; MIP26; CTRCT15
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9603799 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
ATP6V1D, Polyclonal Antibody (Cat# AAA9603799)
Full Name
ATP6V1D Antibody
Gene Names
ATP6V1D; VATD; VMA8; ATP6M
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
PRAS40, Polyclonal Antibody (Cat# AAA9610790)
Full Name
Phospho-PRAS40 (Ser202) Antibody
Gene Names
AKT1S1; Lobe; PRAS40
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Dog
Predicted: Pig, Bovine, Horse, Sheep, Dog
Applications
WB, IHC, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB)
(Western blot analysis of SP1 phosphorylation expression in A549 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
SP1, Polyclonal Antibody (Cat# AAA9600893)
Full Name
Phospho-SP1 (Thr453) Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of GPR40 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
GPR40, Polyclonal Antibody (Cat# AAA9604259)
Full Name
GPR40 Antibody
Gene Names
FFAR1; FFA1R; GPR40; GPCR40
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9601126 at 1/100 staining human brain tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 39 degree C)
14-3-3 zeta/delta, Polyclonal Antibody (Cat# AAA9601126)
Full Name
Phospho-14-3-3 zeta/delta (Thr232) Antibody
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of UBE2R2 expression in Jurkat whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
UBE2R2, Polyclonal Antibody (Cat# AAA9603798)
Full Name
UBE2R2 Antibody
Gene Names
UBE2R2; UBC3B; CDC34B; E2-CDC34B
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
