$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
Western Blot (WB) (Western Blot on Non-stimulated A431 cell lysate probed withMBS600408.)
Western Blot (WB) (Western blot analysis of Raftlin expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.)
Western Blot (WB) (Western blot analysis of ACTN2 expression in HeLa (A); mouse kidney (B); H9C2 (C) whole cell lysates.)
Western Blot (WB) (ACTN2 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ACTN2 antibody detected the ACTN2 protein (arrow).)
Western Blot (WB) (Western Blot detection against Immunogen (36.45kD).)
Western Blot (WB) (Western Blot analysis of MACF1 expression in transfected 293T cell line by MACF1 monoclonal antibody. Lane 1: MACF1 transfected lysate (10.45kD). Lane 2: Non-transfected lysate.)
Testing Data (Detection limit for recombinant GST tagged MACF1 is 0.03ng/ml as a capture antibody.)
Western Blot (WB) (Western Blot analysis of ACTN1 expression in transfected 293T cell line by ACTN1 polyclonal antibody. Lane 1: ACTN1 transfected lysate (103.1kD). Lane 2: Non-transfected lysate.)
Testing Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between ACTN1 and CAMK2A. HeLa cells were stained with ACTN1 rabbit purified polyclonal 1:1200 and CAMK2A mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Western Blot (WB) (Western blot analysis of ACTN4 Antibody (C-term) in T47D, A2058, A375, HepG2 cell line and mouse bladder tissue lysates (35ug/lane). ACTN4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ACTN4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of MDA-231 cells using ACTN4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of extracts from Hela cells(serum starvation treatment), using Phospho-Tau (Thr492/Thr175) Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC) (Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Western Blot (WB) (Western Blot on Non-stimulated A431 cell lysate probed withMBS600408.)
Western Blot (WB) (ACTN2 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ACTN2 antibody detected the ACTN2 protein (arrow).)
Western Blot (WB) (Western blot analysis of alpha-actinin in human A431 cells (lanes 1 & 3) and rabbit spleen fibroblasts (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-alpha-actinin 4 (a.a. 2-11) or mouse monoclonal anti-alpha-actinin (C-terminal region).)
Western Blot (WB) (Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS629597. ACTN1 (arrow) was detected usingMBS629597.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human breast carcinoma usingMBS629597, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629597 for immunohistochemistry.)
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of MDA-231 cells using MBS629597 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of alpha-actinin 4 in A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1,2,5.6) or after immunoprecipitation using alpha-actinin (C-terminal region) antibody in the absence (lanes 3 & 7) or presence of pervanadate-treated A431 cell lysate (lanes 4 & 8). Some lanes of the blot were treated with alkaline phosphatase (lanes 2 & 6). The blots were probed with anti-alpha-actinin (C-terminal region) or anti-alpha-actinin 4 (Tyr-4).)
Testing Data (Detection limit for recombinant GST tagged MACF1 is approximately 1ng/ml as a capture antibody.)
Western Blot (WB) (Western Blot on Non-stimulated A431 cell lysate probed withMBS600408.)
Testing Data (Detection limit for recombinant GST tagged ACTN4 is 0.3 ng/ml as a capture antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human Breast Carrcinoma Tissue using a-actinin Mouse mAb diluted at 1:200.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human Kidney Tissue using a-actinin Mouse mAb diluted at 1:200.)
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