Rabbit anti-Human ACTN4 Polyclonal Antibody | anti-ACTN4 antibody

ACTN4, CT (Alpha-actinin-4, Non-muscle alpha-actinin 4, F-actin Cross-linking Protein) (MaxLight 490)

Cat. Num. AAA6461252

• Gene Names: ACTN4; FSGS; FSGS1; ACTININ-4
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ACTN4; Polyclonal Antibody; CT (Alpha-actinin-4; Non-muscle alpha-actinin 4; F-actin Cross-linking Protein) (MaxLight 490); anti-ACTN4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACTN4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-ACTN4 antibody: FLISA, Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: FLISA: 1:1,000; WB: 1:50-1:100; FC/FACS: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ACTN4 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ACTN4 Antibody (C-term) in T47D, A2058, A375, HepG2 cell line and mouse bladder tissue lysates (35ug/lane). ACTN4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ACTN4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using ACTN4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ACTN4 antibody

Antibodies; Actin Related Proteins

References

1. Kikuchi, S., Clin. Cancer Res. 14(17), 5348-5356 (2008). 2. Barbolina, M.V., Lab. Invest. 88(6), 602-614 (2008). 3. Kimura, M., Intern. Med. 47(12), 1099-1106 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 12025678
• NCBI GeneID: 81
• NCBI Accession #: NP_004915
• NCBI GenBank Nucleotide #: NM_004924.5
• UniProt Accession #: O43707; O76048; A4K467; D6PXK4
• Molecular Weight: 59,579 Da
• NCBI Official Full Name: alpha-actinin-4 isoform 1
• NCBI Official Synonym Full Names: actinin alpha 4
• NCBI Official Symbol: ACTN4
• NCBI Official Synonym Symbols: FSGS; FSGS1; ACTININ-4
• NCBI Protein Information: alpha-actinin-4
• UniProt Protein Name: Alpha-actinin-4
• Protein Family: Alpha-actinin
• UniProt Gene Name: ACTN4
• UniProt Entry Name: ACTN4_HUMAN

NCBI Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTN4: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the alpha-actinin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: cortical cytoskeleton; extracellular space; neuron projection; focal adhesion; protein complex; perinuclear region of cytoplasm; cytoplasm; stress fiber; extracellular region; intercellular junction; pseudopodium; Z disc; ribonucleoprotein complex; nucleus

Molecular Function: actin filament binding; integrin binding; protein binding; protein homodimerization activity; ligand-dependent nuclear receptor transcription coactivator activity; retinoic acid receptor binding; nucleoside binding; protein N-terminus binding; calcium ion binding; actin binding; nuclear hormone receptor binding

Biological Process: regulation of apoptosis; retinoic acid receptor signaling pathway; platelet activation; actin filament bundle formation; protein transport; platelet degranulation; response to hypoxia; negative regulation of cell motility; positive regulation of cell motility; positive regulation of sodium:hydrogen antiporter activity; positive regulation of pinocytosis; blood coagulation

Disease: Focal Segmental Glomerulosclerosis 1