Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1639 results for " AKT1 Antibody" - showing 550-600
Western Blot (WB)
(Western blot analysis of Phospho-AKT1 (S129) expression in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate (MBS1750189).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AKT1 )
AKT1, Monoclonal Antibody (Cat# AAA1750189)
Full Name
Anti-Phospho-AKT1 (S129) Rabbit Monoclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
AKT1, Monoclonal Antibody (Cat# AAA8528467)
Full Name
Mouse Monoclonal Antibody to AKT1
Applications
WB, ICC, FC/FACS
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Protein Kinase B Alpha, Monoclonal Antibody (Cat# AAA147005)
Full Name
Mouse Anti Human Protein Kinase B Alpha
Reactivity
Human
Applications
EIA, WB
Purity
Purified from mouse ascetic fluids by protein-A affinity chromatography
Pricing
$250/0.02 mg | $560/0.1 mg | $180/0.005 mg | $2,300/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
Protein Kinase B Alpha (PKBa), ELISA Kit (Cat# AAA2704298)
Full Name
Mouse Protein Kinase B Alpha (PKBa) ELISA Kit
Gene Names
Akt1; Akt; PKB; Rac; LTR-akt; PKB/Akt; PKBalpha
Reactivity
Mouse
Pricing
$330/48-Strip-Wells | $415/96-Strip-Wells | $1,665/5x96-Strip-Wells | $3,205/10x96-Strip-Wells | $245/24-Strip-Wells
SDS-Page
(Recombinant AKT1 protein gel 10% SDS-PAGE Coomassie staining MW: 60.5 kDa Purity: ?95%)
AKT1, Recombinant Protein (Cat# AAA389202)
Full Name
Recombinant AKT1 protein
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Pricing
$580/0.02 mg | $4,280/1 mg | $19,215/5x1 mg
Western Blot (WB)
(Western blot analysis of extracts from Mouse brain, using Phospho-Dnmt1 (Ser714) Antibody. The lane on the left was treated with blocking peptide.)
Dnmt1, Polyclonal Antibody (Cat# AAA9612760)
Full Name
Phospho-Dnmt1 (Ser714) Antibody
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using Akt Antibody. The picture on the right is blocked with the synthesized peptide.)
Akt, Polyclonal Antibody (Cat# AAA9503686)
Full Name
Akt Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 5', using AKT1 (Phospho-Thr308) Antibody. The lane on the right is blocked with the phospho peptide.)
AKT1, Polyclonal Antibody (Cat# AAA9503424)
Full Name
AKT1 (Phospho-Thr308) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells using Rabbit anti AKT1 (pThr450) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with either Insulin (100nM) for 10 minutes or EGF (100ng/ml) for 30 minutes)
AKT1, Polyclonal Antibody (Cat# AAA225185)
Full Name
Rabbit anti AKT1 (pThr450)
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human
Applications
WB
Purity
Purified whole serum by affinity chromatography.
Pricing
$430/0.05 mL | $1,780/5x0.05 mL
Western Blot (WB)
(Western blot analysis of FOXA2 expression in HepG2 cell lysate (MBS179530).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FOXA2 )
FOXA2/Hnf3B, Monoclonal Antibody (Cat# AAA179530)
Full Name
Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody
Gene Names
FOXA2; HNF3B; TCF3B
Reactivity
Human
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001270)
Full Name
AKT1 Antibody: PerCP
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using AKT1 (Phospho-Thr450) antibody)
AKT1, Polyclonal Antibody (Cat# AAA5309626)
Full Name
AKT1 antibody
Applications
IF, IHC, WB
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Western Blot (WB)
(Western blot analysis of lysates from mouse liver, using Akt (Phospho-Tyr326) Antibody. The lane on the right is blocked with the phospho peptide.)
Akt, Polyclonal Antibody (Cat# AAA9502678)
Full Name
Akt (Phospho-Tyr326) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKT1/2/3 Antibody. The picture on the right is blocked with the synthesized peptide.)
AKT1/2/3, Polyclonal Antibody (Cat# AAA9504345)
Full Name
AKT1/2/3 Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Akt1, Polyclonal Antibody (Cat# AAA8520457)
Full Name
Akt1 (phospho Tyr474) Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using AKT1/2/3 (Phospho-Tyr315/316/312) Antibody. The picture on the right is blocked with the phospho peptide.)
AKT1/2/3, Polyclonal Antibody (Cat# AAA9503236)
Full Name
AKT1/2/3 (Phospho-Tyr315/316/312) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(Western blot analysis of AKT (pT72) expression in HepG2 (A), Hela (B) whole cell lysates.)
AKT, Polyclonal Antibody (Cat# AAA8304181)
Full Name
Anti-AKT (pT72) Phospho Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat, Bovine, Pig, Sheep
Applications
WB, IHC
Purity
Purified by immunogen affinity chromatography.
Pricing
$185/0.03 mL | $275/0.1 mL | $380/0.2 mL | $1,650/5x0.2 mL
Western Blot (WB)
(Western blot analysis of PRAS40 expression in 293T cell lysate (MBS179976).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1S1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AKT1S1 )
PRAS40, Monoclonal Antibody (Cat# AAA179976)
Full Name
Anti-PRAS40 Rabbit Monoclonal Antibody
Gene Names
AKT1S1; Lobe; PRAS40
Reactivity
Human, Mouse
Applications
FC/FACS, IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from A549 cells, using AKT1/3 Antibody. The lane on the right is blocked with the synthesized peptide.)
AKT1/3, Polyclonal Antibody (Cat# AAA9504346)
Full Name
AKT1/3 Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001268)
Full Name
AKT1 Antibody: HRP
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001255)
Full Name
AKT1 Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001271)
Full Name
AKT1 Antibody: RPE
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001257)
Full Name
AKT1 Antibody: ATTO 488
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate (MBS1750190).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AKT1 )
AKT1, Monoclonal Antibody (Cat# AAA1750190)
Full Name
Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded section of Human Mammary Cancer Tissue)
AKT1/2, Polyclonal Antibody (Cat# AAA5312641)
Full Name
AKT1/2 Antibody
Gene Names
Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; DAKT; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3
Applications
WB, IHC
Purity
Affinity Chromatography
Pricing
$660/0.1 mg | $2,965/5x0.1 mg
AKT1, Monoclonal Antibody (Cat# AAA8528468)
Full Name
Mouse Monoclonal Antibody to AKT1
Applications
WB, IHC, FC/FACS
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001266)
Full Name
AKT1 Antibody: Biotin
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001267)
Full Name
AKT1 Antibody: FITC
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells treated with PDGF or vehicle control using Rabbit anti AKT1 (pSer473) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with PDGF)
AKT1, Polyclonal Antibody (Cat# AAA225183)
Full Name
Rabbit anti AKT1 (pSer473)
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Applications
WB
Purity
Purified whole serum by affinity chromatography.
Pricing
$430/0.05 mL | $1,780/5x0.05 mL
Western Blot (WB)
(Western blot analysis of Phospho-AKT1 (T450) expression in (1) 3T3 cell lysate treated with Lambda Phosphatase; (2) 3T3 cell lysate (MBS1750188).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AKT1 )
AKT1, Monoclonal Antibody (Cat# AAA1750188)
Full Name
Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
AKT, Monoclonal Antibody (Cat# AAA8528795)
Full Name
AKT Mouse Monoclonal Antibody (10D6)
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human
Applications
WB, IHC
Purity
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(IB: HEK293 cell lysate was probed with anti-Akt1/PKBa, clone AW24 (1:10,000). )
AKT1, Monoclonal Antibody (Cat# AAA6242388)
Full Name
AKT1 (Rac PKa, PKBa) (APC)
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$940/0.1 mL | $4,230/5x0.1 mL
Testing Data
(HeLa cells were grown to 90% confluency and were stimulated with EGF 200 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Akt P-Thr308 versus untreated HeLa lysates.)
Akt, ELISA Kit (Cat# AAA9511039)
Full Name
Akt (Phospho-Thr308) Phospho Sandwich ELISA Kit
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Pricing
$510/96 Tests | $4,535/10x96 Tests | $2,305/5x96 Tests
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001259)
Full Name
AKT1 Antibody: ATTO 594
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western Blot (WB) analysis of AKT (phospho-S473) polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysateLane2:L02 starved with PBS (1×PBS,PH7. 4) for 1 houre whole cell lysateLane3:L02 starved with PBS (1×PBS,PH7. 4) for 1 houre then treated with DMEM (10%FBS) for 5 minutes whole cell lysateLane4:L02 starved with PBS (1×PBS,PH7. 4) for 1 houre then treated with DMEM (10%FBS) for 10 minutes whole cell lysate)
AKT, Polyclonal Antibody (Cat# AAA3004498)
Full Name
AKT (Phospho-S473) Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Pricing
$285/0.05 mL | $380/0.1 mL | $1,440/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from mouse brain tissue, using Phospho-EZH2(Thr367) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
EZH2, Polyclonal Antibody (Cat# AAA9614525)
Full Name
Phospho-EZH2 (Thr367) Antibody
Gene Names
EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
WB, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Akt1 (Phospho-Thr450) Antibody. The picture on the right is blocked with the phospho peptide.)
Akt1, Polyclonal Antibody (Cat# AAA9502405)
Full Name
Akt1 (Phospho-Thr450) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001264)
Full Name
AKT1 Antibody: Alkaline Phosphatase
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser124) Antibody. The picture on the right is blocked with the phospho peptide.)
Akt, Polyclonal Antibody (Cat# AAA9502406)
Full Name
Akt (Phospho-Ser124) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
AKT, Monoclonal Antibody (Cat# AAA8528799)
Full Name
AKT Mouse Monoclonal Antibody (1H2)
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human
Applications
WB, IHC
Purity
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
AKT, Polyclonal Antibody (Cat# AAA8541483)
Full Name
AKT (Phospho-Ser473) Rabbit pAb
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Unpurified
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-768) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.)
AKT1, Polyclonal Antibody (Cat# AAA8001256)
Full Name
AKT1 Antibody: ATTO 390
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Akt1, Monoclonal Antibody (Cat# AAA8533088)
Full Name
Anti-Akt1 Mouse mAb
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse
Applications
WB
Purity
Affinity purified
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Akt, Monoclonal Antibody (Cat# AAA8528797)
Full Name
Phospho-Akt (S473) Mouse Monoclonal Antibody (6F8)
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human
Applications
WB, IHC
Purity
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western BlotPositive WB detected in?Hela whole cell lysateAll lanes?Phospho-AKT1 antibody at 2.25ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60 KDaObserved band size: 60 KDa)
AKT1, Monoclonal Recombinant Antibody (Cat# AAA7114782)
Full Name
Phospho-AKT1 (T450) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human
Applications
EIA, WB
Purity
Affinity-Chromatography
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
PRAS40, Polyclonal Antibody (Cat# AAA5400785)
Full Name
PRAS40 Antibody BIOTIN
Gene Names
AKT1S1; Lobe; PRAS40
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, IP, WB
Purity
Immobilized antigen affinity chromatography
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Western Blot (WB)
(Western Blot (WB) analysis of p-AKT (S473) pAb at 1:500 dilutionLane1:The Brain tissue lysate of Mouse (40ug) Lane2:The Lung tissue lysate of Rat (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug) Lane5:L02 whole cell lysate (40ug))
AKT, Polyclonal Antibody (Cat# AAA3004497)
Full Name
AKT (Phospho-S473) Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Pricing
$285/0.05 mL | $380/0.1 mL | $1,440/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using AKT1 Antibody.)
AKT1, Monoclonal Antibody (Cat# AAA1751900)
Full Name
Anti-AKT1 Monoclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data
(EGF-treated A431 cells were fixed, permeabilized, and stained with MBS609322 (2ug/ml) (red). Cy3-conjugated anti-mouse was used for detection and Phalloidin-AlexaFluor 488 (green) was used to stain actin.)
AKT, Monoclonal Antibody (Cat# AAA6245132)
Full Name
AKT, phosphorylated (Ser473) (Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1) (MaxLight 405)
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$940/0.1 mL | $4,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Retinoic Acid Receptor α/β (Phospho-Ser96) using TNF-α treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Retinoic Acid Receptor alpha/beta, Polyclonal Antibody (Cat# AAA9614206)
Full Name
Phospho-Retinoic Acid Receptor alpha/beta (Ser96) Antibody
Gene Names
RARA; RAR; NR1B1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
