Mouse anti-Rat, Human CD235a Monoclonal Antibody | anti-CD235a antibody

Anti-CD235a Biotin

Cat. Num. AAA1801882

• Gene Names: GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• Reactivity: Rat, Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Synonyms: CD235a; Monoclonal Antibody; Anti-CD235a Biotin; Glycophorin A; GYPA; GPA; PAS-2; Sialoglycoprotein alpha; MN sialoglycoprotein; GPSAT; GPErik; MSN blood group; glycophorin A (MNS blood group); anti-CD235a antibody

• Host: Mouse
• Reactivity: Rat, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: JC159
• Specificity: The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
• Purity/Purification: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD235a antibody: Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS), Immunohistochemistry-Frozen (IHC-F)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml
• Conjugation: Biotin
• Immunogen: Membrane preparation from splenic hairy cell leukemia
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD235a (JC159) Biotin antibody (concentration in sample 5 ug/ml, Streptavidin APC).)

Flow Cytometry (FC/FACS)

(Separation of human CD235a positive erythrocytes (red-filled) from leukocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD235a (JC159) Biotin antibody (concentration in sample 5 ug/ml, Streptavidin APC).)

Related Product Information for anti-CD235a antibody

CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.

References

*Alijotas-Reig J, Palacio-Garcia C, Llurba E, Vilardell-Tarres M: Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review. Fertil Steril. 2013 Feb;99(2):441-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/23122952,*Maijenburg MW, Kleijer M, Vermeul K, Mul EP, van Alphen FP, van der Schoot CE, Voermans C: The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging. Haematologica. 2012 Feb;97(2):179-83., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=kleijer+m,*Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR: Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients. PLoS One. 2013 Nov 25;8(11):e81002., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=beck+z+ja,*Yamauchi T, Takenaka K, Urata S, Shima T, Kikushige Y, Tokuyama T, Iwamoto C, Nishihara M, Iwasaki H, Miyamoto T, Honma N, Nakao M, Matozaki T, Akashi K: Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment. Blood. 2013 Feb 21;121(8):1316-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/23293079
*Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgidb=p,*Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008;52(2):69-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/18380804ordin,*Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597., URL: https://www.ncbi.nlm.nih.gov/pubmed/8605964dopt=A,*Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990., URL:,*Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994., URL:

NCBI and Uniprot Product Information

• NCBI GI #: 259016238
• NCBI GeneID: 2993
• UniProt Accession #: P02724; Q9BS51; A8K3E6; B8Q182; B8Q185
• Molecular Weight: 13,000 Da
• NCBI Official Full Name: Glycophorin-A
• NCBI Official Synonym Full Names: glycophorin A (MNS blood group)
• NCBI Official Symbol: GYPA
• NCBI Official Synonym Symbols: MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• NCBI Protein Information: glycophorin-A
• UniProt Protein Name: Glycophorin-A
• UniProt Gene Name: GYPA
• UniProt Synonym Gene Names: GPA

NCBI Description

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]

Uniprot Description

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).