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9 results for "calpain-type-cysteine-protease" - showing 1-9
Activity Data
(Figure. The binding activity of CAPN3 with TTN.Calpain 3 is a calcium-dependent cysteine protease mainly expressed in skeletal muscle. In humans, calpain 3 is encoded by the CAPN3 gene. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. Besides, Titin (TTN) has been identified as an interactor of CAPN3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CAPN3 and recombinant human TTN. Briefly, CAPN3 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TTN-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CAPN3 and TTN was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 3 (CAPN3), Active Protein (Cat# AAA2105179)
Full Name
Active Calpain 3 (CAPN3)
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A; LGMDD4; LGMDR1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $485/0.05 mg | $755/0.1 mg | $935/0.2 mg | $1,820/0.5 mg | $2,695/1 mg | $6,585/5 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530).)
Calpain 1, Monoclonal Antibody (Cat# AAA4381357)
Full Name
Calpain 1
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human
Applications
EIA, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).)
Calpastatin, Monoclonal Antibody (Cat# AAA4381359)
Full Name
Calpastatin
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
EIA, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of Calpain 2 using anti-Calpain 2 antibody (MBS1750836).
Calpain 2 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calpain 2 Antibody (MBS1750836) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
)
Calpain 2, Polyclonal Antibody (Cat# AAA1750836)
Full Name
Anti-Calpain 2 Picoband Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Calpain 1 expression in Human fetal kidney lysate (MBS179774).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CAPN1 )
Calpain 1, Monoclonal Antibody (Cat# AAA179774)
Full Name
Anti-Calpain 1 Rabbit Monoclonal Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
ADL1, Polyclonal Antibody (Cat# AAA7143160)
Full Name
Rabbit anti-Oryza sativa subsp. japonica (Rice) ADL1 Polyclonal Antibody
Reactivity
Oryza sativa subsp. japonica (Rice)
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
DEK1, Polyclonal Antibody (Cat# AAA7170209)
Full Name
Rabbit anti-Zea mays (Maize) DEK1 Polyclonal Antibody
Reactivity
Zea mays (Maize)
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
DEK1, Polyclonal Antibody (Cat# AAA7195146)
Full Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DEK1 Polyclonal Antibody
Reactivity
Arabidopsis thaliana (Mouse-ear cress)
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
Calpastatin, Polyclonal Antibody (Cat# AAA9629067)
Full Name
Calpastatin Antibody
Gene Names
CAST; BS-17
Reactivity
Human, Mouse, Rat
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
