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199 results for " Neurology process" - showing 1-50


Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells, using GAPDH Antibody )

GAPDH, Monoclonal Antibody (Cat# AAA179205)

Full Name
Anti-GAPDH Rabbit Monoclonal Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human, Mouse, Rat
Applications
FC, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot detection of Fatty Acid Synthase in Hela, C6, 3T3, CHO-K1 and MCF7 cell lysates using Fatty Acid Synthase mouse mAb (dilution 1:500).Predicted band size:273kDa.Observed band size:273kDa.)

Fatty Acid Synthase, Monoclonal Antibody (Cat# AAA475287)

Full Name
Anti-Fatty Acid Synthase Mouse mAb
Gene Names
FASN; FAS; OA-519; SDR27X1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IP, IF
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot detection of Wnt3a in MCF-7, ZR75-1, MDA-MB-468 and T47D cell lysates using Wnt3a mouse mAb (1:1000 diluted).Predicted band size: 42KDa.Observed band size:42KDa.)

Wnt3a, Monoclonal Antibody (Cat# AAA475532)

Full Name
Anti-Wnt3a Mouse mAb
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (MBS1750387).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse lung tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: rat spleen tissue lysates,Lane 4: human A549 whole cell lysates,Lane 5: human placenta tissue lysates,Lane 6: human Hela whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3 / GST pi antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GST3 / GST pi at approximately 23KD. The expected band size for GST3 / GST pi is at 23KD. )

GST3/GST pi, Polyclonal Antibody (Cat# AAA1750387)

Full Name
Anti-GST3/GST pi Picoband Antibody
Gene Names
Gstp1; GstpiB
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3beta mouse mAb (1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.)

GSK3 beta, Monoclonal Antibody (Cat# AAA475481)

Full Name
Anti-GSK3 beta Mouse mAb
Reactivity
Human, Mouse
Applications
WB, IF, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (MBS1750890).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: mouse brain tissue lysates,Lane 4: 293T whole Cell lysates,Lane 5: 293T whole Cell lysates,Lane 6: HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Musashi 1/Msi1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Musashi 1/Msi1 at approximately 39KD. The expected band size for Musashi 1/Msi1 is at 39KD. )

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA1750890)

Full Name
Anti-Musashi 1/Msi1 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of CD105 using anti-CD105 antibody (MBS1750808).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD105 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD105 at approximately 95-105KD. The expected band size for CD105 is at 71KD. )

CD105, Polyclonal Antibody (Cat# AAA1750808)

Full Name
Anti-CD105 Picoband Antibody
Gene Names
Eng; Endo; CD105; AI528660; AI662476; S-endoglin
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of PAX5 using anti-PAX5 antibody (MBS1750462).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Raji cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX5 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAX5 at approximately 45KD. The expected band size for PAX5 is at 42KD. )

PAX5/Bsap, Polyclonal Antibody (Cat# AAA1750462)

Full Name
Anti-PAX5/Bsap Picoband antibody
Gene Names
PAX5; ALL3; BSAP
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (MBS1750388).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat lung tissue lysates,Lane 2: rat liver tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: mouse liver tissue lysates,Lane 5: mouse spleen tissue lysates,Lane 6: human Hela whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3 / GST pi antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GST3 / GST pi at approximately 23KD. The expected band size for GST3 / GST pi is at 23KD. )

GST3/GST pi, Polyclonal Antibody (Cat# AAA1750388)

Full Name
Anti-GST3/GST pi Picoband Antibody
Gene Names
Gstp1; Gst3; Gstp; GST-P; Gstp2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of PON1 using anti-PON1 antibody (MBS1750425). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse Liver tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PON1 at approximately 43KD. The expected band size for PON1 is at 40KD. )

PON1/Paraoxonase 1, Polyclonal Antibody (Cat# AAA1750425)

Full Name
Anti-PON1/Paraoxonase 1 Picoband Antibody
Gene Names
Pon1; Pon
Reactivity
Mouse
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of GAD65 using anti-GAD65 antibody (MBS1750815).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD65 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GAD65 at approximately 65KD. The expected band size for GAD65 is at 65KD. )

GAD65, Polyclonal Antibody (Cat# AAA1750815)

Full Name
Anti-GAD65 Picoband antibody
Gene Names
GAD2; GAD65
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of VEGFB using anti-VEGFB antibody (MBS1750874).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFB antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VEGFB at approximately 29KD. The expected band size for VEGFB is at 22KD. )

VEGFB, Polyclonal Antibody (Cat# AAA1750874)

Full Name
Anti-VEGFB Picoband antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of VCAM1 using anti-VCAM1 antibody (MBS1750592).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse spleen tissue lysates,Lane 2: mouse lung tissue lysates,Lane 3: rat spleen tissue lysates,Lane 4: rat lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VCAM1 at approximately 110KD. The expected band size for VCAM1 is at 81KD. )

VCAM1/Cd106, Polyclonal Antibody (Cat# AAA1750592)

Full Name
Anti-VCAM1/Cd106 Picoband Antibody
Gene Names
Vcam1; CD106; Vcam-1
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)

Nkx2.2, Polyclonal Antibody (Cat# AAA542222)

Full Name
Nkx2.2 Antibody
Gene Names
NKX2-2; NKX2B; NKX2.2
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunohistochemistry (IHC) (Figure 1. IHC analysis of AGRP using anti-AGRP antibody (MBS1750364).AGRP was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AGRP Antibody (MBS1750364) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

AGRP/Art, Polyclonal Antibody (Cat# AAA1750364)

Full Name
Anti-AGRP/Art Picoband antibody
Gene Names
Agrp; Art; Agrt
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of TSPAN12 using anti-TSPAN12 antibody (MBS1750898).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela cell lysates,Lane 2: human SW620 cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPAN12 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSPAN12 at approximately 43KD. The expected band size for TSPAN12 is at 35KD. )

TSPAN12, Polyclonal Antibody (Cat# AAA1750898)

Full Name
Anti-TSPAN12 Picoband antibody
Gene Names
TSPAN12; EVR5; NET2; NET-2; TM4SF12
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of HSPB8/Hsp22 using anti- HSPB8/Hsp22 antibody (MBS1750773).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat skeletal muscle tissue lysates,Lane 2: rat heart tissue lysates,Lane 3: mouse skeletal muscle tissue lysates,Lane 4: mouse heart tissue lysates,Lane 5: HELA whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- HSPB8/Hsp22 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPB8/Hsp22 at approximately 22KD. The expected band size for HSPB8/Hsp22 is at 22KD. )

HSPB8/Hsp22, Polyclonal Antibody (Cat# AAA1750773)

Full Name
Anti-HSPB8/Hsp22 Picoband Antibody
Gene Names
HSPB8; H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of 14-3-3 expression in Hela lysate (MBS179850).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAB )

14-3-3, Monoclonal Antibody (Cat# AAA179850)

Full Name
Anti-14-3-3 Rabbit Monoclonal Antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot detection of Fyn in Hela, 3T3, C6, COS7, CEM, Ramos and Jurkat cell lysates using Fyn mouse mAb (1:500 diluted).Predicted band size:59KDa.Observed band size:59KDa.)

Fyn, Monoclonal Antibody (Cat# AAA475169)

Full Name
Anti-Fyn Mouse mAb
Gene Names
FYN; SLK; SYN; p59-FYN
Reactivity
Human, Monkey, Rat, Mouse
Applications
WB, ICC
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
ELISA (Indirect ELISA assay for anti-Ferritin Light Chain mouse mAb.Antigen coating concentration: 4ug/ml.)

Ferritin Light Chain, Monoclonal Antibody (Cat# AAA475160)

Full Name
Anti-Ferritin Light Chain Mouse mAb
Gene Names
FTL; LFTD; NBIA3
Reactivity
Human
Applications
EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of 14-3-3 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate; (3) PC-12 cell lysate (MBS179851).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAB )

14-3-3, Monoclonal Antibody (Cat# AAA179851)

Full Name
Anti-14-3-3 Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot detection of LC3A in CHO-K1 cell lysate(B)and CHO-K1 transfected by LC3A (A)cell lysate using LC3A mouse mAb (1:2000 diluted).)

LC3A, Monoclonal Antibody (Cat# AAA475151)

Full Name
LC3A
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Transfected
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human kidney, using ARG1 Antibody(MBS179560)ARG1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARG1 Antibody (MBS179560)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(MBS176451) with DAB as the chromogen. )

ARG1/Arginase 1, Monoclonal Antibody (Cat# AAA179560)

Full Name
Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot detection of OLIG2 in CHO-K1 cell lysate (B)and CHO-K1 transfected by EGFP-OLIG2 fragment (A)cell lysate using OLIG2 mouse mAb (1:1000 diluted).)

OLIG2, Monoclonal Antibody (Cat# AAA475207)

Full Name
Anti-OLIG2 Mouse mAb
Gene Names
OLIG2; BHLHB1; OLIGO2; RACK17; PRKCBP2; bHLHe19
Reactivity
Transfected
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of 14-3-3 alpha + beta expression in Hela lysate (MBS179852).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAB )

14-3-3 alpha + beta, Monoclonal Antibody (Cat# AAA179852)

Full Name
Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of 14-3-3 sigma expression in A431 cell lysate (MBS179566).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFN )

14-3-3 sigma, Monoclonal Antibody (Cat# AAA179566)

Full Name
Anti-14-3-3 sigma Rabbit Monoclonal Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Ubiquitin expression in Jurkat cell lysate (MBS179903).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UBB )

K48-linkage Specific Ubiquitin, Monoclonal Antibody (Cat# AAA179903)

Full Name
Anti-K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody
Gene Names
UBB; HEL-S-50
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of 14-3-3 epsilon expression in 293T cell lysate (MBS179716).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAE monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAE )

14-3-3 epsilon, Monoclonal Antibody (Cat# AAA179716)

Full Name
Anti-14-3-3 epsilon Rabbit Monoclonal Antibody
Gene Names
YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of LRP1 expression in A549 cell lysate (MBS179466).)

LRP1/Lrp 1 Cluster Ii, Monoclonal Antibody (Cat# AAA179466)

Full Name
Anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal Antibody
Gene Names
LRP1; APR; KPA; LRP; A2MR; CD91; APOER; LRP1A; TGFBR5; IGFBP3R; IGFBP-3R; IGFBP3R1
Reactivity
Human, Mouse, Rat
Applications
FC, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot detection of PPARgamma fragment in CHO-K1 cell lysate (A) and CHO-K1 transfected by pEGFP-C1-PPARgamma (B) cell lysate using HER2/ErbB2 mouse mAb (1:500 diluted).Predicted band size: 53kDa.Observed band size:53kDa.)

PPARgamma, Monoclonal Antibody (Cat# AAA475424)

Full Name
Anti-PPARgamma Mouse mAb
Gene Names
PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
Reactivity
Transfected
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
ELISA (Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml.)

Leptin, Monoclonal Antibody (Cat# AAA475196)

Full Name
Anti-Leptin Mouse mAb
Gene Names
LEP; OB; OBS; LEPD
Reactivity
Human
Applications
EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of ADAM17 expression in Hela cell lysate (MBS179386).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAM17 )

ADAM17/Tace, Monoclonal Antibody (Cat# AAA179386)

Full Name
Anti-ADAM17/Tace Rabbit Monoclonal Antibody
Gene Names
ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of active Caspase-3 expression in Jurkat cell lysate treated with Camptothecin (MBS179271).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CASP3 )

active Caspase-3, Monoclonal Antibody (Cat# AAA179271)

Full Name
Anti-active Caspase-3 Rabbit Monoclonal Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human (Predicted Reactivity: Hamster)
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
ELISA (Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml.)

Leptin, Monoclonal Antibody (Cat# AAA475159)

Full Name
Anti-Leptin Mouse mAb
Gene Names
LEP; OB; OBS; LEPD
Reactivity
Human
Applications
EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of Tau expression in Mouse rat brain lysate (MBS179098).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPT monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPT )

Tau, Monoclonal Antibody (Cat# AAA179098)

Full Name
Anti-Tau Rabbit Monoclonal Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of GST expression in GST recombinant protein (MBS179306).)

GST, Monoclonal Antibody (Cat# AAA179306)

Full Name
Anti-GST Rabbit Monoclonal Antibody
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Reactivity
All Species
Applications
IP, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Ubiquitin expression in K63-linked-Ub2 recombinant protein (MBS179904).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UBB )

K63-linkage Specific Ubiquitin, Monoclonal Antibody (Cat# AAA179904)

Full Name
Anti-K63-linkage Specific Ubiquitin Rabbit Monoclonal Antibody
Gene Names
UBB; HEL-S-50
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

Presenilin 1, Polyclonal Antibody (Cat# AAA544431)

Full Name
Presenilin 1 Antibody Affinity Purified
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182
Reactivity
Bovine, Cat, Human, Monkey, Pig
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody(MBS1750219)CDK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (MBS1750219)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Cdk1/2, Monoclonal Antibody (Cat# AAA1750219)

Full Name
Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human, Mouse, Rat
Applications
IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data (Observed Ferritin Light Chain levels in Human serum samples at different dilution.)

Ferritin Light Chain, Monoclonal Antibody (Cat# AAA477198)

Full Name
Anti-Ferritin Light Chain Mouse mAb
Gene Names
FTL; LFTD; NBIA3
Reactivity
Human
Applications
EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of 14-3-3 gamma expression in HeLa cell lysate (MBS1750006).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAG monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAG )

14-3-3 gamma, Monoclonal Antibody (Cat# AAA1750006)

Full Name
Anti-14-3-3 gamma Rabbit Monoclonal Antibody
Gene Names
YWHAG; EIEE56; PPP1R170; 14-3-3GAMMA
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)

Dystroglycan, ELISA Kit (Cat# AAA1751136)

Full Name
Human Dystroglycan PicoKine ELISA Kit
Gene Names
DAG1; A3a; DAG; AGRNR; 156DAG; MDDGA9; MDDGC7; MDDGC9
Reactivity
Human
There is no detectable cross-reactivity with other relevant proteins.
Applications
EIA
Pricing
$460/96-Strip-Wells | $2,060/5x96-Strip-Wells
Western Blot (WB) (Western blot analysis of SOX1 expression in HeLa cell lysate (MBS1750109).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX1 )

SOX1, Monoclonal Antibody (Cat# AAA1750109)

Full Name
Anti-SOX1 Rabbit Monoclonal Antibody
Reactivity
Predicted Reactivity: Bovine
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

Presenilin 1, Polyclonal Antibody (Cat# AAA544430)

Full Name
Presenilin 1 Antibody Affinity Purified
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182
Reactivity
Bovine, Cat, Human, Monkey, Pig
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB) (Western blot analysis of GBA expression in U87-MG cell lysate.)

GBA/Glucosylceramidase, Monoclonal Antibody (Cat# AAA179574)

Full Name
Anti-GBA/Glucosylceramidase Rabbit Monoclonal Antibody
Gene Names
GBA; GCB; GBA1; GLUC
Reactivity
Human, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)

BDNF, ELISA Kit (Cat# AAA1751015)

Full Name
Dog Canine BDNF PicoKine ELISA Kit
Reactivity
Canine
There is no detectable cross-reactivity with other relevant proteins.
Applications
EIA
Pricing
$460/96-Strip-Wells | $2,060/5x96-Strip-Wells
Western Blot (WB) (Western blot analysis of FASN expression in A549 cell lysate (MBS179494).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FASN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FASN )

FASN/Fatty Acid Synthase, Monoclonal Antibody (Cat# AAA179494)

Full Name
Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody
Gene Names
FASN; FAS; OA-519; SDR27X1
Reactivity
Human, Mouse, Rat
Applications
FC, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

Nkx2.2, Polyclonal Antibody (Cat# AAA542223)

Full Name
Nkx2.2 Antibody Affinity Purified
Gene Names
NKX2-2; NKX2B; NKX2.2
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of ADAM17 using anti-ADAM17 antibody (MBS1750443).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse thymus tissue lysates,Lane 2: human Hela whole cell lysates,Lane 3: human placenta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAM17 at approximately 78KD. The expected band size for ADAM17 is at 97KD. )

ADAM17/Tace, Polyclonal Antibody (Cat# AAA1750443)

Full Name
Anti-ADAM17/Tace Picoband Antibody
Gene Names
ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of PAX6 expression in Human brain lysate (MBS179234).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAX6 )

PAX6, Monoclonal Antibody (Cat# AAA179234)

Full Name
Anti-PAX6 Rabbit Monoclonal Antibody
Gene Names
PAX6; AN; AN2; FVH1; MGDA; WAGR; ASGD5; D11S812E
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
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