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Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Rabbit anti-Mouse SFRP2 Polyclonal Antibody | anti-SFRP2 antibody

SFRP2 Antibody: FITC

Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
Western Blot
Purity
Peptide Affinity Purified
Synonyms
SFRP2; Polyclonal Antibody; SFRP2 Antibody: FITC; Rabbit Anti-Human SFRP2 Polyclonal; SDF5 Antibody; Secreted frizzled-related protein 2 Antibody; FRP 2 Antibody; UNQ361/PRO697 Antibody; sFRP-2 Antibody; SFRP2 Antibody; FKSG12 Antibody; SFRP2_HUMAN Antibody; MGC153618 Antibody; Frizzled related protein 2 Antibody; MGC53344 Antibody; FRP2 Antibody; FRP-2 Antibody; SARP1 Antibody; AI851596 Antibody; SARP 1 Antibody; MGC128911 Antibody; SARP-1 Antibody; Secreted apoptosis related protein 1 Antibody; Secreted apoptosis-related protein 1 Antibody; SDF 5 Antibody; Sfrp2 protein Antibody; sFRP 2 Antibody; Secreted frizzled related protein 2 Antibody; anti-SFRP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
Detects 37 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
295
Applicable Applications for anti-SFRP2 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Target
SFRP2
Immunogen
Synthetic peptide from the C-terminal of Human Secreted frizzled-related protein 2 (aa. 195-295)
Immunogen Species
Human
Cellular Localization
Secreted
Conjugate
FITC
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Tissue Specificity
Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.
Preparation and Storage
Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Western Blot (WB)

(Western blot analysis of Mouse Brain showing detection of 33.5 kDa SFRP2 protein using Rabbit Anti-SFRP2 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 33.5 kDa. Other Band(s): 37 kDa.)

Western Blot (WB) (Western blot analysis of Mouse Brain showing detection of 33.5 kDa SFRP2 protein using Rabbit Anti-SFRP2 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 33.5 kDa. Other Band(s): 37 kDa.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,490 Da
NCBI Official Full Name
secreted frizzled-related protein 2
NCBI Official Synonym Full Names
secreted frizzled related protein 2
NCBI Official Symbol
SFRP2
NCBI Official Synonym Symbols
FRP-2; SARP1; SDF-5
NCBI Protein Information
secreted frizzled-related protein 2
UniProt Protein Name
Secreted frizzled-related protein 2
UniProt Gene Name
SFRP2
UniProt Synonym Gene Names
FRP2; SARP1; FRP-2; sFRP-2; SARP-1

NCBI Description

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Research Articles on SFRP2

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Product Notes

The SFRP2 sfrp2 (Catalog #AAA8001591) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SFRP2 Antibody: FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SFRP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the SFRP2 sfrp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFRP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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