Immunohistochemistry (IHC) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Mouse antibody was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of extracts from various samples, using p38 MAPK Monoclonal Antibody. Lane 1: Mouse brain, blocked with antigen-specific peptides, Lane 2: Mouse brain, Lane 3: Rat brain, Lane 4: HepG2 cells.)
Chemical Structure (Chemical structure of PJ-34 (SIH-366), a PARP inhibitor. CAS #: 344458-15-7. Molecular Formula: C17H17N3O2 *HCL * H2O. Molecular Weight: 331.8 g/mol.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PI3K P85 a/y/beta antibody (left) or the same antibody preincubated with with blocking peptide (right))
Chemical Structure (Chemical structure of C16 (SIH-498), a PKR kinase inhibitor. CAS #: 608512-97-6. Molecular Formula: C13H8N4OS. Molecular Weight: 268.3 g/mol.)
Chemical Structure (Chemical structure of Imatinib mesylate (SIH-453), a BCR-ABL kinase inhibitor. CAS #: 152459-95-5. Molecular Formula: C30H35N7O4S. Molecular Weight: 589.7 g/mol.)
Chemical Structure (Chemical structure of Rapamycin (SIH-212), a mTOR inhibitor. CAS #: 53123-88-9. Molecular Formula: C51H79NO13 . Molecular Weight: 914.18 g/mol.)
Western Blot (Western Blot analysis using SRC antibodyWestern Blot showing SRC antibody used against truncated SRC-His recombinant protein (1) and PMA treated THP-1 cell lysate (2).)
Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: H1299) using a 10 % SDS PAGE gel and TS antibody at a dilution of 1:1000)
Chemical Structure (Chemical structure of Apigenin (SIH-249), a Protein kinase inhibitor. CAS #: 520-36-5. Molecular Formula: C15H10O5. Molecular Weight: 270.2 g/mol.)
Chemical Structure (Chemical structure of Carfilzomib (SIH-544), a 20S Proteasome inhibitor. CAS #: 868540-17-4. Molecular Formula: C40H57N5O7. Molecular Weight: 719.9 g/mol.)
Testing Data (PP242 is a novel potent and selective mTOR inhibitor with an IC50 of 8 nM. PP242 also shows residual (micromolar) activity against panel of tyrosine kinases. But PP242 was much less active against other PI3K family members with IC50 of 2, 2.2, 0.1, 1.3 and 0.41 uM for p110alpha, p110beta, p110delta, p110gamma and DNA-PK, respectively. PP242 inhibits other PI 3-Kinases only at much higher concentrations. PP242, but not rapamycin, causes death of mouse and human leukemia cells. In vivo, PP242 delays leukemia onset and augments the effects of the current front-line tyrosine kinase inhibitors more effectively than does rapamycin. PP242 was also more effective than rapamycin in achieving cytoreduction and apoptosis in multiple myeloma cells. In addition, PP242 was an effective agent against primary multiple myeloma cells in vitro and growth of 8226 cells in mice. Knockdown of the TORC2 complex protein, rictor, was deleterious to multiple myeloma cells further supporting TORC2 as the critical target for PP242. [1][2][3])
Chemical Structure (Chemical structure of PP2 (SIH-470), a Src family kinase inhibitor. CAS #: 172889-27-9. Molecular Formula: C15H16ClN5. Molecular Weight: 301.8 g/mol.)
Chemical Structure (Chemical structure of SB431542 (SIH-511), a ALK4,5 and 7 kinase inhibitor. CAS #: 301836-41-9. Molecular Formula: C22H16N4O3. Molecular Weight: 384.4 g/mol.)
Chemical Structure (Chemical structure of Gefitinib (SIH-445), a EGFR-Kinase inhibitor. CAS #: 184475-35-2. Molecular Formula: C22H24ClFN4O3. Molecular Weight: 446.9 g/mol.)
Structure (Analytical DataHNMR: Consistent with structureHPLC: Shows 98.1% purity)
Western Blot (WB) (Western blot analysis of Matriptase expression with Jurkat cell lysate.)
Chemical Structure (Chemical structure of Dasatinib (SIH-440), a Src and Bcr/Abl kinase inhibitor. CAS #: 302962-49-8. Molecular Formula: C22H26ClN7O2S. Molecular Weight: 488 g/mol.)
Chemical Structure (Chemical structure of SU 4312 (SIH-481), a VEGFR / PDGFR kinase inhibitor. CAS #: 5812-07-7. Molecular Formula: C17H16N2O. Molecular Weight: 264.3 g/mol.)
Chemical Structure (Chemical structure of BSI 201 (SIH-539), a PARP1 inhibitor. CAS #: 160003-66-7. Molecular Formula: C7H5IN2O3. Molecular Weight: 292 g/mol.)
Testing Data (PHA-680632 is the first representative of a new class of Aurora inhibitors (Aurora A/B/C IC50 at 27, 135 and 120 nM, respectively). This agent has a high potential for further development as an anticancer therapeutic. PHA-680632 is active on a wide range of cancer cell lines and shows significant tumor growth inhibition in different animal tumor models at well-tolerated doses. The IC50 of PHA-680632 for cell anti-proliferation ranged from 0.11 uM to 5.6 uM. [1])
Chemical Structure (Chemical structure of Staurosporine (SIH-253), a Protein kinase inhibitor. CAS #: 62996-74-1. Molecular Formula: C28H26N4O3. Molecular Weight: 466.54 g/mol.)
Chemical Structure (Chemical structure of Axitinib (SIH-494), a VEGFR kinase inhibitor. CAS #: 319460-85-0. Molecular Formula: C22H18N4OS. Molecular Weight: 386.5 g/mol.)
Chemical Structure (Chemical structure of PP1 (SIH-469), a Src family kinase inhibitor. CAS #: 172889-26-8. Molecular Formula: C16H19N5. Molecular Weight: 281.4 g/mol.)
Chemical Structure (Chemical structure of Sphingosine (SIH-202), a Protein Kinase C inhibitor. CAS #: 123-78-4. Molecular Formula: C18H37NO2. Molecular Weight: 299.49 g/mol.)
Chemical Structure (Chemical structure of PD98059 (SIH-466), a MEK kinase inhibitor. CAS #: 167869-21-8. Molecular Formula: C16H13NO3. Molecular Weight: 267.3 g/mol.)
Chemical Structure (Chemical structure of Pazopanib (SIH-506), a Multi target receptor tyrosine kinase inhibitor. CAS #: 44473-52-6. Molecular Formula: C21H23N7O2S. Molecular Weight: 437.5 g/mol.)
Chemical Structure (Chemical structure of AG 1478 (SIH-425), a EGFR kinase inhibitor. CAS #: 175178-82-2. Molecular Formula: C16H14ClN3O2. Molecular Weight: 315.8 g/mol.)
Chemical Structure (Chemical structure of Midostaurin (SIH-468), a Protein kinase inhibitor (pan spec.). CAS #: 120685-11-2. Molecular Formula: C35H30N4O4. Molecular Weight: 570.6 g/mol.)
Chemical Structure
Western Blot (WB) (Western blot analysis of Interferon beta expression in HeLa cell lysate (MBS179791).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFNB1 )
Chemical Structure (Chemical structure of KT5720 (SIH-457), a PKA kinase inhibitor. CAS #: 108068-98-0. Molecular Formula: C32H31N3O5. Molecular Weight: 537.6 g/mol.)
Chemical Structure (Chemical structure of Nilotinib (SIH-463), a BCR-ABL kinase inhibitor. CAS #: 641571-10-0. Molecular Formula: C28H22F3N7O. Molecular Weight: 529.5 g/mol.)
Chemical Structure (Chemical structure of Bexarotene (SIH-535), a Retinoid RXR agonist. CAS #: 153559-49-0. Molecular Formula: C24H28O2. Molecular Weight: 348.5 g/mol.)
Chemical Structure (Chemical structure of Lactacystin (SIH-327), a Proteasome inhibitor. CAS #: 133343-34-7. Molecular Formula: C15H24N2O7S. Molecular Weight: 376.4 g/mol.)
Chemical Structure (Chemical structure of GW5074 (SIH-451), a c-Raf1 kinase inhibitor. CAS #: 220904-83-6. Molecular Formula: C15H8Br2INO2. Molecular Weight: 520.9 g/mol.)
Chemical Structure (Chemical structure of LFM A13 (SIH-461), a BTK kinase inhibitor. CAS #: 62004-35-7. Molecular Formula: C11H8Br2N2O2. Molecular Weight: 360 g/mol.)
Chemical Structure (Chemical structure of WHI P131 (SIH-485), a JAK3 kinase inhibitor. CAS #: 202475-60-3. Molecular Formula: C16H15N3O3. Molecular Weight: 297.3 g/mol.)
Chemical Structure (Chemical structure of BML 277 (SIH-436), a CHK2 kinase inhibitor. CAS #: 516480-79-8. Molecular Formula: C20H14ClN3O2. Molecular Weight: 363.8 g/mol.)
Chemical Structure (Chemical structure of BRD 7389 (SIH-497), a p90 RSK kinase inhibitor. CAS #: 376382-11-5. Molecular Formula: C24H18N2O2. Molecular Weight: 366.4 g/mol.)
Chemical Structure (Chemical structure of AG 879 (SIH-429), a NGFR kinase inhibitor. CAS #: 148741-30-4. Molecular Formula: C18H24N2OS. Molecular Weight: 316.5 g/mol.)
Chemical Structure (Chemical structure of SR 3576 (SIH-513), a JNK3 kinase inhibitor. CAS #: 1164153-22-3. Molecular Formula: C27H27N5O5. Molecular Weight: 501.5 g/mol.)
Chemical Structure (Chemical structure of CID 2011756 (SIH-438), a PKD kinase inhibitor. CAS #: 638156-11-3. Molecular Formula: C22H21ClN2O3. Molecular Weight: 396.9 g/mol.)
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