Rabbit anti-Human Interleukin 1 Receptor AcP Polyclonal Antibody | anti-Il1rap antibody

Interleukin 1 Receptor AcP (IL1RacP, IL-1 Receptor Accessory Protein)

Cat. Num. AAA610189

• Gene Names: Il1rap; AI255955; AV239853; IL-1RAcP; 6430709H04Rik
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: Interleukin 1 Receptor AcP; Polyclonal Antibody; Interleukin 1 Receptor AcP (IL1RacP; IL-1 Receptor Accessory Protein); Anti -Interleukin 1 Receptor AcP (IL1RacP; anti-Il1rap antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IL-1RAcP. No crossreactivity to other members in the IL-1 receptor family.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-Il1rap antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot and Immunocytochemistry.; Dilution: Western Blot: 1ug/ml; Immunocytochemistry: 5ug/ml. Detects a band at ~66kD with whole HeLa lysates
• Immunogen: Synthetic peptide corresponding to amino acids near the carboxy terminus of human IL-1RAcP, which differs from the sequence of mouse origin by three amino acids.
• Positive Control: HeLa whole lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HeLa whole cell lysate with MBS610189 (1ug/ml).)

Immunocytochemistry (ICC)

(Immunocytochemistry in HeLa cells with MBS610189 (5ug/ml).)

Related Product Information for anti-Il1rap antibody

The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP (2). IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP (3,4). Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

Product Categories/Family for anti-Il1rap antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 34222514
• NCBI GeneID: 16180
• UniProt Accession #: Q61730; Q8VCB9; Q3UEL3; Q3UVZ1; Q3V2X3
• Molecular Weight: 65,741 Da
• NCBI Official Full Name: Interleukin-1 receptor accessory protein
• NCBI Official Synonym Full Names: interleukin 1 receptor accessory protein
• NCBI Official Symbol: Il1rap
• NCBI Official Synonym Symbols: AI255955; AV239853; IL-1RAcP; 6430709H04Rik
• NCBI Protein Information: interleukin-1 receptor accessory protein; IL-1 receptor accessory protein
• UniProt Protein Name: Interleukin-1 receptor accessory protein
• Protein Family: Interleukin-1 receptor accessory protein
• UniProt Gene Name: Il1rap
• UniProt Entry Name: IL1AP_MOUSE

Uniprot Description

IL1RAP: Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: protein complex; membrane; cytoplasm; extracellular region; plasma membrane; integral to membrane

Molecular Function: signal transducer activity; protein binding; interleukin-1 receptor binding; interleukin-1 receptor activity

Biological Process: interleukin-2 biosynthetic process; immune system process; cytokine and chemokine mediated signaling pathway; innate immune response; inflammatory response; signal transduction