SDS-Page
SDHAF2 recombinant protein
SDHAF2, 30-166aa, Human, His tag, E Coli
Gene Names
SDHAF2; PGL2; SDH5; C11orf79
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
SDHAF2; 30-166aa; Human; His tag; E Coli; Succinate dehydrogenase assembly factor 2 mitochondrial; Succinate dehydrogenase assembly factor 2; mitochondrial; C11orf79; PGL2; SDH5; SDHAF2 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSSFRRFYR GDSPTDSQKD MIEIPLPPWQ ERTDESIETK RARLLYESRK RGMLENCILL SLFAKEHLQH MTEKQLNLYD RLINEPSNDW DIYYWATEAK PAPEIFENEV MALLRDFAKN KNKEQRLRAP DLEYLFEKPR
Sequence Length
166
Applicable Applications for SDHAF2 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for SDHAF2 recombinant protein
SDHAF2 is a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. Defects in SDHAF2 are the cause of hereditary paragangliomas type 2 (PGL2), also known as familial non-chromaffin paragangliomas type 2. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. Recombinant human SDHAF2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for SDHAF2 recombinant protein
References
Kunst,H.P., et al. (2011) Clin. Cancer Res. 17 (2), 247-254; Starker,L.F., et al. (2010) Endocrine 38 (3), 397-401
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19.0kDa (160aa), confirmed by MALDI-TOF
NCBI Official Full Name
succinate dehydrogenase assembly factor 2, mitochondrial
NCBI Official Synonym Full Names
succinate dehydrogenase complex assembly factor 2
NCBI Official Symbol
SDHAF2
NCBI Official Synonym Symbols
PGL2; SDH5; C11orf79
NCBI Protein Information
succinate dehydrogenase assembly factor 2, mitochondrial
UniProt Protein Name
Succinate dehydrogenase assembly factor 2, mitochondrial
Protein Family
UniProt Gene Name
SDHAF2
UniProt Synonym Gene Names
SDH assembly factor 2; SDHAF2
NCBI Description
This gene encodes a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. Mutations in this gene are associated with paraganglioma.[provided by RefSeq, Jul 2010]
Uniprot Description
Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit SDHA of the SDH catalytic dimer.
Research Articles on SDHAF2
Similar Products
Product Notes
The SDHAF2 sdhaf2 (Catalog #AAA205178) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's SDHAF2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SDHAF2 sdhaf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSSFRRFYR GDSPTDSQKD MIEIPLPPWQ ERTDESIETK RARLLYESRK RGMLENCILL SLFAKEHLQH MTEKQLNLYD RLINEPSNDW DIYYWATEAK PAPEIFENEV MALLRDFAKN KNKEQRLRAP DLEYLFEKPR. It is sometimes possible for the material contained within the vial of "SDHAF2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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