Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SDHAF2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 10s.)
Rabbit anti-Human, Mouse SDHAF2 Polyclonal Antibody | anti-SDHAF2 antibody
SDHAF2 Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SDHAF2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SDHAF2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 10s.)
NCBI and Uniprot Product Information
Observed: 15kDa
NCBI Description
This gene encodes a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. Mutations in this gene are associated with paraganglioma.[provided by RefSeq, Jul 2010]
Uniprot Description
SDH5: a subunit of succinate dehydrogenase (SDH) complex II of the mitochondrial electron transport chain. SDH5 is required for insertion of FAD cofactor into SDHA, the catalytic subunit of succinate dehydrogenase (SDH) complex II. SDH is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Mutated in some cancers. Germline mutations cause hereditary paragangliomas type 2, or non-chromaffin paragangliomas type 2. Paragangliomas are rare tumors that arise from components of the neuroendocrine system.
Chromosomal Location of Human Ortholog: 11q12.2
Cellular Component: mitochondrion; mitochondrial matrix
Molecular Function: protein binding
Biological Process: mitochondrial electron transport, succinate to ubiquinone; protein-FAD linkage; protein amino acid dephosphorylation
Disease: Paragangliomas 2
Research Articles on SDHAF2
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Product Notes
The SDHAF2 sdhaf2 (Catalog #AAA9140779) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SDHAF2 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SDHAF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Recommended Dilutions: WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SDHAF2 sdhaf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDHAF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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