SDF2 recombinant protein

SDF2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120597

• Purity: >95% as determined by SDS-PAGE
• Synonyms: SDF2; SDF2 Protein; Human; Recombinant (His Tag); Human SDF2 Protein (His Tag); stromal cell-derived factor 2; SDF2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0, 0.5mM PMSF, 0.5mM EDTA, 0.5mM TCEP, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information i
• Sequence: Met1-Leu211

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the pro form of human SDF2 (NP_008854.2)(Met 1-Leu 211) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SDF2 recombinant protein

Background: Stromal derived factors (SDFs) are a loosely defined group of molecules that are generated by stromal cells. Two of the stromal derived factors, SDF-1 and SDF-4 belong to the chemokine family. Other SDFs, such as SDF-2 and SDF-5 are not well defined and their biological functions are less known. SDF-2 is first isolated from the mouse stromal cell line ST2 as a secretory protein. The amino acid sequence deduced from the murine clone and the human homolog are conserved more than 92 %, and the aa sequence of SDF-2 shows similarity to those of yeast dolichyl phosphate-D-mannose, protein mannosyltransferases. SDF-1 and its receptor are strongly indicated in the progression of various cancers including breast cancer. SDF-2, SDF2-L1, SDF-4, and SDF-5 are ubiquitously expressed in various cancer cell lines and SDF-2, SDF-4 and SDF-5 are expressed in mammary tissues. These SDFs have prognostic value and warrant further investigation in their biological functions and clinical value.

References

Hamada,T.etal.,1996,Gene.176(1-2):211-214. Anjard,C.etal.,1998,Development.125(20):4067-4075. Kang,H.etal.,2009,Int J Oncol.35(1):205-211.

NCBI and Uniprot Product Information

• NCBI GI #: 14141195
• NCBI GeneID: 6388
• NCBI Accession #: NP_008854.2
• NCBI GenBank Nucleotide #: NM_006923.3
• Molecular Weight: 23,026 Da
• NCBI Official Full Name: stromal cell-derived factor 2
• NCBI Official Synonym Full Names: stromal cell-derived factor 2
• NCBI Official Symbol: SDF2
• NCBI Protein Information: stromal cell-derived factor 2; SDF-2
• Protein Family: Stromal cell-derived factor

NCBI Description

The protein encoded by this gene is believed to be a secretory protein. It has regions of similarity to hydrophilic segments of yeast mannosyltransferases. Its expression is ubiquitous and the gene appears to be relatively conserved among mammals. Alternate splicing results in both coding and non-coding variants. A pseudogene of this gene is located on chromosome 15. [provided by RefSeq, Dec 2011]