SCYL1 recombinant protein

SCYL1 recombinant protein

Cat. Num. AAA515280

• Gene Names: SCYL1; GKLP; NKTL; NTKL; P105; TAPK; TEIF; TRAP; HT019
• Applications: Western Blot
• Synonyms: SCYL1; SCYL1 recombinant protein; GKLP; HT019; NKTL; NTKL; P105; TAPK; TEIF; TRAP

• Host: Sf9 insect cells
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: 1-556
• Sequence Length: 2667

• Applicable Applications for SCYL1 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Recombinant human SCYL1 (1-556) was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for SCYL1 recombinant protein

Recombinant human SCYL1 (1-556) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: SCYL1 is a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. SCYL1 has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. SCYL1 activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. SCYL1 forms multimers following transfection into COS-7 cells (1). A SCYL1 binding protein has been identified that co-localized with SCYL1 in cytoplasm and shows ubiquitous expression (2).

Product Categories/Family for SCYL1 recombinant protein

Cellular Proteins; Signaling Proteins - Cellular Proteins

References

1. Kato, M. et.al: Identification and characterization of the human protein kinase-like gene NTKL: mitosis-specific centrosomal localization of an alternatively spliced isoform. Genomics 79: 760-767, 2002.
2. Di, Y. et.al: Cloning and characterization of a novel gene which encodes a protein interacting with the mitosis-associated kinase-like protein NTKL. J. Hum. Genet. 48: 315-321, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 115430240
• NCBI GeneID: 57410
• NCBI Accession #: NP_065731.3
• NCBI GenBank Nucleotide #: NM_020680
• UniProt Accession #: Q96KG9; Q96G50; Q96KG8; Q96KH1; Q9HAW5; Q9HBL3; Q9NR53; A6NJF1
• Molecular Weight: ~85 kDa
• NCBI Official Full Name: Homo sapiens SCY1-like 1 (S. cerevisiae) (SCYL1), transcript variant A, mRNA
• NCBI Official Synonym Full Names: SCY1-like 1 (S. cerevisiae)
• NCBI Official Symbol: SCYL1
• NCBI Official Synonym Symbols: GKLP; NKTL; NTKL; P105; TAPK; TEIF; TRAP; HT019
• NCBI Protein Information: N-terminal kinase-like protein; SCY1-like protein 1; teratoma-associated tyrosine kinase; telomerase regulation-associated protein; coated vesicle-associated kinase of 90 kDa; telomerase transcriptional element-interacting factor; telomerase transcriptional elements-interacting factor; likely ortholog of mouse N-terminal kinase-like protein
• UniProt Protein Name: N-terminal kinase-like protein
• Protein Family: N-terminal kinase-like protein
• UniProt Gene Name: SCYL1
• UniProt Synonym Gene Names: CVAK90; GKLP; NTKL; TAPK; TEIF; TRAP
• UniProt Entry Name: NTKL_HUMAN

NCBI Description

This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro. Ref.8 Ref.11Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region. Ref.8 Ref.11

Subunit structure: Interacts with GORAB. Interacts with COPA, COPB1 and COPB2

By similarity. Homooligomer. Interacts with AP2B1. Ref.1 Ref.10

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus › cis-Golgi network. Note: Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner. Ref.2 Ref.11Isoform 1: Cytoplasm. Note: Cytoplasmic throughout the cell cycle. Ref.2 Ref.11Isoform 2: Cytoplasm. Note: Cytoplasmic throughout the cell cycle. Ref.2 Ref.11Isoform 3: Cytoplasm. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Note: Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner. Ref.2 Ref.11Isoform 6: Nucleus Ref.2 Ref.11.

Tissue specificity: Ubiquitous. Ref.1

Domain: The protein kinase domain is predicted to be catalytically inactive.

Sequence similarities: Belongs to the protein kinase superfamily.Contains 3 HEAT repeats.Contains 1 protein kinase domain.

Sequence caution: The sequence AAG09726.1 differs from that shown. Reason: Frameshift at positions 289 and 307. The sequence AAG17902.1 differs from that shown. Reason: Frameshift at several positions.