Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ADCY3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
Rabbit ADCY3 Polyclonal Antibody | anti-ADCY3 antibody
ADCY3 Polyclonal Antibody
IHC 1:50 - 1:200
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ADCY3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ADCY3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes adenylyl cyclase 3 which is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This protein appears to be widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes. [provided by RefSeq, Jul 2008]
Uniprot Description
ADCY3: a membrane-associated enzyme that catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes. Involved in odorant detection (possibly) via modulation of intracellular cAMP concentration.
Protein type: Transporter; Membrane protein, multi-pass; EC 4.6.1.1; Transporter, aquaporin family; Lyase; Adenylyl cyclase; Membrane protein, integral; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 2p23.3
Cellular Component: membrane; integral to plasma membrane; cytoplasm; plasma membrane; cilium
Molecular Function: calmodulin binding; metal ion binding; ATP binding; adenylate cyclase activity
Biological Process: epidermal growth factor receptor signaling pathway; synaptic transmission; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; phospholipase C activation; water transport; activation of protein kinase A; sensory perception of smell; energy reserve metabolic process; innate immune response; renal water homeostasis; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; signal transduction; G-protein signaling, adenylate cyclase activating pathway; transmembrane transport
Research Articles on ADCY3
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Product Notes
The ADCY3 adcy3 (Catalog #AAA9128184) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADCY3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADCY3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB 1:500 - 1:2000 IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the ADCY3 adcy3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADCY3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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