RFXANK recombinant protein

RFXANK, 1-237aa, Human, His tag, E Coli

Cat. Num. AAA205412

• Gene Names: RFXANK; BLS; RFX-B; ANKRA1; F14150_1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: RFXANK; 1-237aa; Human; His tag; E Coli; DNA-binding protein RFXANK isoform b; ANKRA1; BLS; F14150_1; RFX-B; RFXANK recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol.
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMELTQPA EDLIQTQQTP ASELGDPEDP GEEAADGSDT VVLSLFPCTP EPVNPEPDAS VSSPQGSSLK HSTTLTNRQR GNEVSALPAT LDCDNLVNKP DERGFTPLIW ASAFGEIETV RFLLEWGADP HILAKERESA LSLASTGGYT DIVGLLLERD VDINIYDWNG GTPLLYAVRG NHVKCVEALL ARGADLTTEA DSGYTPMDLA VALGYRKVQQ VIENHILKLF QSNLVPADPE
• Sequence Length: 237

• Applicable Applications for RFXANK recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RFXANK recombinant protein

RFXANK, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Two transcript variants encoding different isoforms have been described for this gene, with only one isoform showing activation activity. Recombinant human RFXANK protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RFXANK recombinant protein

Immunology

References

Krawczyk M, Masternak K, et al. (2005). Mol Cell Biol. 25 (19):8607-18.

NCBI and Uniprot Product Information

• NCBI GI #: 19924156
• NCBI GeneID: 8625
• NCBI Accession #: NP_604389
• NCBI GenBank Nucleotide #: NM_134440.2
• UniProt Accession #: O14593; O95839; Q24JQ1; Q6FGA8
• Molecular Weight: 28 kDa (260aa), confirmed by MALDI-TOF
• NCBI Official Full Name: DNA-binding protein RFXANK isoform b
• NCBI Official Synonym Full Names: regulatory factor X associated ankyrin containing protein
• NCBI Official Symbol: RFXANK
• NCBI Official Synonym Symbols: BLS; RFX-B; ANKRA1; F14150_1
• NCBI Protein Information: DNA-binding protein RFXANK
• UniProt Protein Name: DNA-binding protein RFXANK
• Protein Family: DNA-binding protein
• UniProt Gene Name: RFXANK
• UniProt Synonym Gene Names: ANKRA1; RFXB; RFX-B

NCBI Description

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed:9806546, PubMed:10072068, PubMed:10725724). May also potentiate the activation of RAF1 ().