Spermidine/Spermine N1-Acetyltransferase 1 Recombinant Protein | SAT1 recombinant protein

Recombinant Spermidine/Spermine N1-Acetyltransferase 1 (SAT1)

Cat. Num. AAA2033103

• Gene Names: SAT1; SAT; DC21; KFSD; SSAT; KFSDX; SSAT-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Spermidine/Spermine N1-Acetyltransferase 1; Recombinant Spermidine/Spermine N1-Acetyltransferase 1 (SAT1); DC21; KFSD; SAT; SSAT; SSAT-1; Diamine Acetyltransferase 1; Polyamine N-acetyltransferase 1; Putrescine acetyltransferase; SAT1 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MAKFKIRPAT ASDCSDILRL IKELAKYEYM EDQVVLTEKD LLEDGFGEHP FYHCLVAEVP KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMKC RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMAAE E
• Sequence Length: 171

• Applicable Applications for SAT1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 23.7kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Glu171
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 14250050
• NCBI GeneID: 6303
• Molecular Weight: 20,024 Da
• NCBI Official Full Name: Spermidine/spermine N1-acetyltransferase 1
• NCBI Official Synonym Full Names: spermidine/spermine N1-acetyltransferase 1
• NCBI Official Symbol: SAT1
• NCBI Official Synonym Symbols: SAT; DC21; KFSD; SSAT; KFSDX; SSAT-1
• NCBI Protein Information: diamine acetyltransferase 1
• UniProt Protein Name: Diamine acetyltransferase 1
• Protein Family: Serine acetyltransferase
• UniProt Gene Name: SAT1
• UniProt Synonym Gene Names: SAT; SSAT; SSAT-1
• UniProt Entry Name: SAT1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the acetyltransferase family, and is a rate-limiting enzyme in the catabolic pathway of polyamine metabolism. It catalyzes the acetylation of spermidine and spermine, and is involved in the regulation of the intracellular concentration of polyamines and their transport out of cells. Defects in this gene are associated with keratosis follicularis spinulosa decalvans (KFSD). Alternatively spliced transcripts have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

SAT: Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine. Defects in SAT1 may be a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX). A rare disorder affecting the skin and the eye. Affected men show thickening of the skin of the neck, ears, and extremities, especially the palms and soles, loss of eyebrows, eyelashes and beard, thickening of the eyelids with blepharitis and ectropion, and corneal degeneration. Belongs to the acetyltransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; Acetyltransferase; EC 2.3.1.57

Chromosomal Location of Human Ortholog: Xp22.1

Cellular Component: cytosol; intracellular

Molecular Function: diamine N-acetyltransferase activity; protein binding

Biological Process: angiogenesis; polyamine biosynthetic process; polyamine metabolic process; putrescine catabolic process; regulation of cell proliferation

Disease: Keratosis Follicularis Spinulosa Decalvans, X-linked