Testing Data
(Purified Dog IgG detected with Mouse anti Dog IgG2 followed by Goat anti Mouse IgG1:HRP )
Mouse anti-Dog IgG2 Monoclonal Antibody | anti-IgG2 antibody
MOUSE ANTI DOG IgG2
Reactivity
Dog
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Synonyms
IgG2; Monoclonal Antibody; MOUSE ANTI DOG IgG2; anti-IgG2 antibody
Host
Mouse
Reactivity
Dog
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CA4F1
Specificity
IgG2
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-IgG2 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
ELISA: 1/15,000; 1/150,000
Reactivity Notes
Goat, Human, Rabbit, Sheep
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch
Shelf Life: 18 months from date of despatch
Testing Data
(Purified Dog IgG detected with Mouse anti Dog IgG2 followed by Goat anti Mouse IgG1:HRP )
Testing Data
(Purified Dog IgG detected with Mouse anti Dog IgG2 followed by Goat anti Mouse IgG1:HRP )
Related Product Information for anti-IgG2 antibody
Mouse anti Dog IgG2 antibody, clone CA4F1 recognizes the canine IgG2 heavy chain. ELISA tests have shown that this clone does not react with canine IgG1, IgA or IgM (Arce et al. 2002). Mouse anti Dog IgG2, clone CA3H1 does not react with the Fab portion of canine IgG or recognize heat, acid and periodate treated canine IgG. Competition ELISA demonstrated that anti dog IgG2 clones CA3H1 and CA4F1 were able to completely inhibit the binding of each other to IgG2 indicating that both clones recognize the same or closely related epitopes.
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Product Notes
The IgG2 (Catalog #AAA224778) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI DOG IgG2 reacts with Dog and may cross-react with other species as described in the data sheet. AAA Biotech's IgG2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB). ELISA: 1/15,000; 1/150,000. Researchers should empirically determine the suitability of the IgG2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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