Testing Data
SARS-CoV Recombinant Protein | PLpro recombinant protein
SARS-CoV (strain WH20) Plpro/papain-like protease (His Tag)
Purity
>90 % as determined by SDS-PAGE.
Synonyms
SARS-CoV; SARS-CoV (strain WH20) Plpro/papain-like protease (His Tag); PLpro recombinant protein
Host
E Coli
Purity/Purification
>90 % as determined by SDS-PAGE.
Form/Format
Lyophilized from sterile 50 mM Tris, 0.1% Tween, pH9.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Sequence Positions
325aa
Sequence
Glu1541-Tyr1859
Species
SARS
Tag
C-His
Predicted N Terminal
Glu1541
Protein Construction
|A DNA sequence encoding the SARS-CoV (strain WH20) papain-like protease (PLpro) (AAX16193.1) (Glu1541-Tyr1859) was expressed with a polyhistidine tag at the C-terminus.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping: They are shipped out with blue ice pack
Shipping: They are shipped out with blue ice pack
Testing Data
Testing Data
Related Product Information for PLpro recombinant protein
The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.
References
Yahira M. Baez-Santos.The SARS-coronavirus papain-like protease: Structure, function and inhibition by designed antiviral compounds. Antiviral Research.2015
NCBI and Uniprot Product Information
Molecular Weight
The recombinant SARS-CoV (strain WH20) papain-like protease (PLpro) consists of 325 amino acids and predicts a molecular mass of 36.65kDa.
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Product Notes
The PLpro (Catalog #AAA8118803) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 325aa. The amino acid sequence is listed below: Glu1541-Ty r1859. It is sometimes possible for the material contained within the vial of "SARS-CoV, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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