Western Blot (WB)
(Western Blot analysis of various cells using Cyclin E1 Polyclonal Antibody at dilution of 1:500.)
Rabbit Cyclin E1 Polyclonal Antibody | anti-CCNE1 antibody
Cyclin E1 Polyclonal Antibody
Western Blot (WB)
(Western Blot analysis of various cells using Cyclin E1 Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of various cells using Cyclin E1 Polyclonal Antibody at dilution of 1:500.)
Immunofluorescence (IF)
(Immunofluorescence analysis of Rat spleen tissue using Cyclin E1 Polyclonal Antibody at dilution of 1:200.)
Immunofluorescence (IF)
(Immunofluorescence analysis of Rat spleen tissue using Cyclin E1 Polyclonal Antibody at dilution of 1:200.)
NCBI and Uniprot Product Information
Observed MW: 49
NCBI Description
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]
Uniprot Description
CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.
Protein type: Activator; Nuclear receptor co-regulator; Cell cycle regulation
Chromosomal Location of Human Ortholog: 19q12
Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytosol; nucleoplasm; nucleus
Molecular Function: androgen receptor binding; cyclin-dependent protein kinase regulator activity; kinase activity; protein binding; protein complex binding; protein kinase binding; transcription coactivator activity
Biological Process: androgen receptor signaling pathway; antral ovarian follicle growth; cell division; cellular response to nutrient; DNA replication initiation; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; liver development; mitotic cell cycle; organ regeneration; positive regulation of cell differentiation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cell cycle; regulation of protein kinase activity; response to corticosterone stimulus; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to ethanol; response to methylmercury; response to progesterone stimulus; response to purine; response to vitamin E; Wnt receptor signaling pathway
Research Articles on CCNE1
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Product Notes
The CCNE1 ccne1 (Catalog #AAA2535843) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cyclin E1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin E1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:100, ELISA: 1:5000-1:20000. Researchers should empirically determine the suitability of the CCNE1 ccne1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin E1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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