Monkey S100 Calcium Binding Protein P ELISA Kit | S100P elisa kit

Monkey S100 Calcium Binding Protein P ELISA Kit

Cat. Num. AAA742934

• Gene Names: S100P; MIG9
• Reactivity: Monkey
• Synonyms: S100 Calcium Binding Protein P; Monkey S100 Calcium Binding Protein P ELISA Kit; S100P elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This S100P ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of monkey S100P. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100P elisa kit

Principle of the Assay: S100P ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100P antibody and an S100P-HRP conjugate. The assay sample and buffer are incubated together with S100P-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100P concentration since S100P from samples and S100P-HRP conjugate compete for the anti-S100P antibody binding site. Since the number of sites is limited, as more sites are occupied by S100P from the sample, fewer sites are left to bind S100P-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100P concentration in each sample is interpolated from this standard curve.

Product Categories/Family for S100P elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119602790
• NCBI GeneID: 6286
• Molecular Weight: 10,400 Da
• NCBI Official Full Name: S100 calcium binding protein P
• NCBI Official Synonym Full Names: S100 calcium binding protein P
• NCBI Official Symbol: S100P
• NCBI Official Synonym Symbols: MIG9
• NCBI Protein Information: protein S100-P; protein S100-E; S100 calcium-binding protein P; migration-inducing gene 9 protein
• UniProt Protein Name: Protein S100-P
• Protein Family: Protein
• UniProt Gene Name: S100P
• UniProt Synonym Gene Names: S100E; MIG9
• UniProt Entry Name: S100P_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

S100P: Homodimer. Interacts with S100PBP and S100Z. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: microvillus membrane; cytoplasm; nucleus

Molecular Function: protein binding; RAGE receptor binding; magnesium ion binding; calcium ion binding; calcium-dependent protein binding

Biological Process: response to organic substance; endothelial cell migration