Human S100 calcium binding protein A4 ELISA Kit | S100A4 elisa kit

Human Protein S100-A4, S100A4 ELISA Kit

Cat. Num. AAA915435

• Gene Names: S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
• Reactivity: Human
• Synonyms: S100 calcium binding protein A4; Human Protein S100-A4; S100A4 ELISA Kit; Human Protein S100-A4 (S100A4) ELISA kit; 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; OTTHUMP00000015469; OTTHUMP00000032895; S100 calcium binding protein A4 (calcium protein; calvasculin; metastasin; murine placental homolog) ; S100 calcium-bind; S100A4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human S100A4. No significant cross-reactivity or interference between human S100A4 and analogues was observed.
• Sequence Length: 101

• Samples: Serum, plasma, cell culture supernates, tissue homogenates.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 0.9 ng/ml-60 ng/ml.
• Sensitivity: 0.225 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4506765
• NCBI GeneID: 6275
• NCBI Accession #: NP_002952.1
• NCBI GenBank Nucleotide #: NM_002961.2
• UniProt Accession #: P26447; Q6ICP8; A8K7R8; D3DV46
• Molecular Weight: 11,729 Da
• NCBI Official Full Name: protein S100-A4
• NCBI Official Synonym Full Names: S100 calcium binding protein A4
• NCBI Official Symbol: S100A4
• NCBI Official Synonym Symbols: 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
• NCBI Protein Information: protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
• UniProt Protein Name: Protein S100-A4
• Protein Family: Protein
• UniProt Gene Name: S100A4
• UniProt Synonym Gene Names: CAPL; MTS1
• UniProt Entry Name: S10A4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; neuron projection; perinuclear region of cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; RAGE receptor binding; calcium ion binding; actin binding; calcium-dependent protein binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; epithelial to mesenchymal transition