Rabbit VAPB Polyclonal Antibody | anti-VAPB antibody

VAPB Antibody

Cat. Num. AAA2526965

• Gene Names: VAPB; ALS8; VAP-B; VAMP-B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antigen affinity purification
• Synonyms: VAPB; Polyclonal Antibody; VAPB Antibody; ALS8; VAP-B; VAMP-B; anti-VAPB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 243

• Applicable Applications for anti-VAPB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant protein of human VAPB
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-48702 / sc-48703
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using VAPB antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using VAPB antibody at dilution of 1:200 (x400 lens))

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using VAPB antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-VAPB antibody

The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.

NCBI and Uniprot Product Information

• NCBI GI #: 37182052
• NCBI GeneID: 9217
• Molecular Weight: 11,152 Da
• NCBI Official Full Name: VAPB
• NCBI Official Synonym Full Names: VAMP (vesicle-associated membrane protein)-associated protein B and C
• NCBI Official Symbol: VAPB
• NCBI Official Synonym Symbols: ALS8; VAP-B; VAMP-B
• NCBI Protein Information: vesicle-associated membrane protein-associated protein B/C; VAMP-associated 33 kDa protein
• UniProt Protein Name: Vesicle-associated membrane protein-associated protein B/C
• Protein Family: Virulence-associated protein
• UniProt Gene Name: VAPB
• UniProt Synonym Gene Names: VAMP-B/VAMP-C; VAMP-associated protein B/C; VAP-B/VAP-C
• UniProt Entry Name: VAPB_HUMAN

NCBI Description

The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq, Jul 2008]

Uniprot Description

VAPB: Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation. Defects in VAPB are the cause of amyotrophic lateral sclerosis type 8 (ALS8). ALS8 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. Defects in VAPB are a cause of spinal muscular atrophy proximal adult autosomal dominant (SMAPAD); also called late-onset spinal muscular atrophy Finkel type. A form of spinal muscular atrophy, a neuromuscular disorder characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAPAD is characterized by proximal muscle weakness that begins in the lower limbs and then progresses to upper limbs, onset in late adulthood (after third decade) and a benign course. Most of the patients remain ambulatory 10 to 40 years after clinical onset. Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; enzyme binding; protein homodimerization activity; FFAT motif binding; protein heterodimerization activity; microtubule binding; beta-tubulin binding

Biological Process: cellular calcium ion homeostasis; endoplasmic reticulum organization and biogenesis; ER to Golgi vesicle-mediated transport; sphingolipid metabolic process; unfolded protein response, activation of signaling protein activity; positive regulation of viral genome replication; sphingolipid biosynthetic process; unfolded protein response; virus-host interaction; negative regulation of viral protein levels in host cell

Disease: Spinal Muscular Atrophy, Late-onset, Finkel Type; Amyotrophic Lateral Sclerosis 8