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SDS-Page

Related To Receptor Tyrosine Kinase (RYK) Recombinant Protein | RYK recombinant protein

Recombinant Related To Receptor Tyrosine Kinase (RYK)

Gene Names
Ryk; Vik; ERK-3; AW536699
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Related To Receptor Tyrosine Kinase (RYK); Recombinant Related To Receptor Tyrosine Kinase (RYK); RYK recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TLRI EKNDLRSVTL LEAKAKVKDI AISRERITLK DVLQEGTFGR IFHGILVDEK DPNKEKQTFV KTVKDQASEV QVTMMLTESC KLRGLHHRNL LPITHVCIEE GEKPMVVLPY MNWGNLKLFL RQCKLVEANN PQAISQQDLV HMAIQIACGM SYLARREVIH RDLAARNCVI DDTLQVKITD NALSRDLFPMDYHCLGDNEN RPVRWMALES LVNNEFSSAS DVWAFGVTLW ELMTLGQTPY VDI
Sequence Length
591
Applicable Applications for RYK recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Thr287~Ile533 (Accession # Q01887) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6.7
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32.1kDa
NCBI Official Full Name
tyrosine-protein kinase RYK isoform 2
NCBI Official Synonym Full Names
receptor-like tyrosine kinase
NCBI Official Symbol
Ryk
NCBI Official Synonym Symbols
Vik; ERK-3; AW536699
NCBI Protein Information
tyrosine-protein kinase RYK; NYK-R; kinase VIK; met-related kinase; growth factor receptor
UniProt Protein Name
Tyrosine-protein kinase RYK
Protein Family
UniProt Gene Name
Ryk
UniProt Synonym Gene Names
Mrk
UniProt Entry Name
RYK_MOUSE

Uniprot Description

RYK: a receptor tyrosine kinase of the Ryk family. Associates with, and may modulate the signaling of, EphB2 and EphB3. Expressed in nestin-positive progenitor cells and neurons, some oligodendrocytes, O-2A progenitor cells, and type-2 astrocytes in developing CNS.

Protein type: Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Ryk family

Cellular Component: membrane; cytoplasm; integral to membrane; plasma membrane; nucleus

Molecular Function: transferase activity; Wnt-protein binding; protein binding; Wnt receptor activity; transmembrane receptor activity; frizzled binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: neuron differentiation; corpus callosum development; axon guidance; Wnt receptor signaling pathway; axonogenesis; positive regulation of MAPKKK cascade; skeletal morphogenesis; negative chemotaxis; signal transduction; negative regulation of axon extension involved in axon guidance; phosphorylation; protein amino acid phosphorylation; neurite development

Research Articles on RYK

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Product Notes

The RYK ryk (Catalog #AAA2018498) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Related To Receptor Tyrosine Kinase (RYK) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the RYK ryk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TLRI EKNDLRSVTL LEAKAKVKDI AISRERITLK DVLQEGTFGR IFHGILVDEK DPNKEKQTFV KTVKDQASEV QVTMMLTESC KLRGLHHRNL LPITHVCIEE GEKPMVVLPY MNWGNLKLFL RQCKLVEANN PQAISQQDLV HMAIQIACGM SYLARREVIH RDLAARNCVI DDTLQVKITD NALSRDLFPM DYHCLGDNEN RPVRWMALES LVNNEFSSAS DVWAFGVTLW ELMTLGQTPY VDI. It is sometimes possible for the material contained within the vial of "Related To Receptor Tyrosine Kinase (RYK), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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