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Testing Data (P-peptide-+)

PP2A-alpha Antibody | anti-PP2A-alpha antibody

PP2A-alpha (Phospho-Tyr307) Antibody

Gene Names
PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
PP2A-alpha; PP2A-alpha (Phospho-Tyr307) Antibody; EC 3.1.3.16; PP2AA; RP-C; Replication protein C; Serine/threonine protein phosphatase 2A; catalytic subunit; alpha isoform; anti-PP2A-alpha antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
PP2A-alpha (Phospho-Tyr307) antibody detects endogenous levels of PP2A-alpha only when phosphorylated at tyrosine 307.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
309
Applicable Applications for anti-PP2A-alpha antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human PP2A-alpha around the phosphorylation site of tyrosine 307 (P-D-YP-F-L).
All Sites
Human: Tyr307
Mouse: Tyr307
Rat: Tyr307
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Testing Data (P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PP2A-alpha (Phospho-Tyr307) antibody. Western blot analysis of extracts from A549 cells, using PP2A-alpha (Phospho-Tyr307) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PP2A-alpha (Phospho-Tyr307) antibody. Western blot analysis of extracts from A549 cells, using PP2A-alpha (Phospho-Tyr307) antibody.)

Immunofluorescence (IF)

(P-peptide-+ Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) antibody.)

Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) antibody.)
References
Wen Chen, Cancer Res., Sep 2005; 65: 8183-8192. Kathy Bonness, Mol. Cancer Ther., Nov 2006; 5: 2727-2736. Stefaan Wera, J. Biol. Chem., Sep 1995; 270: 21374. Todd D. Prickett, J. Biol. Chem., Sep 2004; 279: 38912-38920.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,735 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform isoform 1
NCBI Official Synonym Full Names
protein phosphatase 2 catalytic subunit alpha
NCBI Official Symbol
PPP2CA
NCBI Official Synonym Symbols
RP-C; PP2Ac; PP2CA; PP2Calpha
NCBI Protein Information
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Gene Name
PPP2CA
UniProt Synonym Gene Names
PP2A-alpha; RP-C

NCBI Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Research Articles on PP2A-alpha

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Product Notes

The PP2A-alpha ppp2ca (Catalog #AAA8501337) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The PP2A-alpha (Phospho-Tyr307) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PP2A-alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the PP2A-alpha ppp2ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2A-alpha, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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