Human reticulon 4 ELISA Kit | RTN4 elisa kit

Human Reticulon-4, RTN4 ELISA Kit

Cat. Num. AAA921848

• Gene Names: RTN4; ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• Reactivity: Human
• Synonyms: reticulon 4; Human Reticulon-4; RTN4 ELISA Kit; Human Reticulon-4 (RTN4) ELISA kit; ASY; NI220/250; NOGO; NOGO-A; NOGOC; NSP; NSP-CL; Nbla00271; Nbla10545; Nogo-B; Nogo-C; RTN-X; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; Human NogoA; My043 protein; OTTHUMP00000201714; OTTHUMP00000201801; OTTHU; RTN4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human RTN4. No significant cross-reactivity or interference between human RTN4 and analogues was observed.
• Sequence Length: 199

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 46.88 pg/ml - 3000 pg/ml.
• Sensitivity: The minimum detectable dose of human RTN4 is typically less than 11.7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RTN4 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RTN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RTN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RTN4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RTN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5902016
• NCBI GeneID: 57142
• NCBI Accession #: NP_008939.1
• NCBI GenBank Nucleotide #: NM_007008.2
• UniProt Accession #: Q9NQC3; O94962; Q7L7Q5; Q7L7Q6; Q7L7Q8; Q8IUA4; Q96B16; Q9BXG5; Q9H212; Q9H3I3; Q9UQ42; Q9Y293
• Molecular Weight: 129,931 Da
• NCBI Official Full Name: reticulon-4 isoform C
• NCBI Official Synonym Full Names: reticulon 4
• NCBI Official Symbol: RTN4
• NCBI Official Synonym Symbols: ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• NCBI Protein Information: reticulon-4; foocen; Human NogoA; reticulon 5; My043 protein; neurite outgrowth inhibitor; neurite growth inhibitor 220; neuroendocrine-specific protein C homolog
• UniProt Protein Name: Reticulon-4
• Protein Family: Reticulon
• UniProt Gene Name: RTN4
• UniProt Synonym Gene Names: KIAA0886; NOGO; My043; SP1507; Nogo protein; NSP
• UniProt Entry Name: RTN4_HUMAN

NCBI Description

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Nogo: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; Inhibitor; Membrane protein, integral; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cell projection; cell soma; endoplasmic reticulum; plasma membrane; integral to endoplasmic reticulum membrane; nuclear envelope; intracellular

Molecular Function: protein binding

Biological Process: regulation of apoptosis; nerve growth factor receptor signaling pathway; cerebral cortex radial glia guided migration; apoptosis; axonal fasciculation; negative regulation of axonogenesis; angiogenesis; negative regulation of cell growth; negative regulation of axon extension; regulation of axonogenesis; regulation of cell migration