Rat Reticulon 4 ELISA Kit | RTN4 elisa kit

Rat Reticulon 4 ELISA Kit

Cat. Num. AAA761583

• Gene Names: RTN4; ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• Reactivity: Rat
• Synonyms: Reticulon 4; Rat Reticulon 4 ELISA Kit; RTN4; RTN4 elisa kit

• Reactivity: Rat
• Sequence Length: 1192

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RTN4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat RTN4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat RTN4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat RTN4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat RTN4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 156914657
• NCBI GeneID: 57142
• Molecular Weight: 108,450 Da
• NCBI Official Full Name: Reticulon 4
• NCBI Official Synonym Full Names: reticulon 4
• NCBI Official Symbol: RTN4
• NCBI Official Synonym Symbols: ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• NCBI Protein Information: reticulon-4
• UniProt Protein Name: Reticulon-4
• Protein Family: Reticulon
• UniProt Gene Name: RTN4
• UniProt Synonym Gene Names: KIAA0886; NOGO; Nogo protein; NSP
• UniProt Entry Name: RTN4_HUMAN

NCBI Description

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Nogo: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Cell development/differentiation; Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: endoplasmic reticulum; integral to endoplasmic reticulum membrane; intracellular; nuclear envelope; plasma membrane

Molecular Function: protein binding

Biological Process: apoptosis; axonal fasciculation; axonogenesis; cerebral cortex radial glia guided migration; negative regulation of axon extension; negative regulation of axonogenesis; negative regulation of cell growth; nerve growth factor receptor signaling pathway; regulation of apoptosis; regulation of axonogenesis; regulation of cell migration