R-Spondin-3 Recombinant Protein | RSPO3 recombinant protein

Recombinant Human R-Spondin-3

Cat. Num. AAA146177

• Gene Names: RSPO3; PWTSR; THSD2; CRISTIN1
• Purity: Greater than 95% as determined by SDS PAGE.
• Synonyms: R-Spondin-3; Recombinant Human R-Spondin-3; RSPO3 Human; R-Spondin-3 Human Recombinant; R-spondin-3; Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2; RSPO3; PWTSR; THSD2; CRISTIN1; RSPO3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: Greater than 95% as determined by SDS PAGE.
• Form/Format: RSPO3 was lyophilized from a 0.2 uM filtered solution in PBS, pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: QNASRGRRQR RMHPNVSQGC QGGCATCSDY NGCLSCKPRL FFALERIGMK QIGVCLSSCP
• Sequence Length: 272

• Solubility: It is recommended to quick spin followed by reconstitution of RSPO3 in PBS to a concentration no less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized RSPO3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution RSPO3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Related Product Information for RSPO3 recombinant protein

Description: Recombinant Human R-Spondin-3 produced in HEK293 cells is a polypeptide chain starting at amino acid at position 22 to amino acid at position 200, fused to an FC-6xHis-tag at C-terminus, containing a total of 498 amino acids. RSPO3 is a truncated protein that lacks amino acid V at position 180 to amino acid H at position 251 and purified by proprietary chromatographic techniques.

Introduction: R-spondin-3 (RSPO3) belongs to the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein which is widely expressed in many tissues. RSPO3 contains 2 Furin-like repeats which have been found in various eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases, and one TSP type-1 domain. RSPO3 acts as an activator of the beta-catenin signaling cascade, initiating TCF-dependent gene activation.

Product Categories/Family for RSPO3 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 18490982
• NCBI GeneID: 84870
• NCBI Accession #: NP_116173.2
• NCBI GenBank Nucleotide #: NM_032784.4
• UniProt Accession #: Q9BXY4; Q5VTV4; Q96K87; B2RC27
• Molecular Weight: 33,233 Da
• NCBI Official Full Name: R-spondin-3
• NCBI Official Synonym Full Names: R-spondin 3
• NCBI Official Symbol: RSPO3
• NCBI Official Synonym Symbols: PWTSR; THSD2; CRISTIN1
• NCBI Protein Information: R-spondin-3; R-spondin 3 homolog; protein with TSP type-1 repeat; roof plate-specific spondin-3; thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain containing 2
• UniProt Protein Name: R-spondin-3
• Protein Family: R-spondin
• UniProt Gene Name: RSPO3
• UniProt Synonym Gene Names: PWTSR; THSD2; hPWTSR; hRspo3
• UniProt Entry Name: RSPO3_HUMAN

NCBI Description

This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]

Uniprot Description

R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding; frizzled binding; receptor binding

Biological Process: Wnt receptor signaling pathway through beta-catenin