R-Spondin-3 Active Protein | RSPO3 active protein

Recombinant Human R-Spondin-3 Protein

Cat. Num. AAA2556678

• Gene Names: RSPO3; PWTSR; THSD2; CRISTIN1
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: R-Spondin-3; Recombinant Human R-Spondin-3 Protein; R-spondin-3; RSPO3; Protein with TSP type-1 repeat; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1; RSPO3 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH7.2.
• Sequence Length: 272

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Gln22-His272
• Fusion Tag: C-Fc-6His
• Activity: Measured by its binding ability in a functional ELISA. 2. Immobilized RSPO3-His (146) at 10 mug/mL (100 muL/well) can bind human RNF43-Fc.The EC50 of human RNF43-Fc is 0.01-0.03mug/mL.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

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Related Product Information for RSPO3 active protein

Protein Construction: Recombinant Human R-spondin-3 is produced by our Mammalian expression system and the target gene encoding Gln22-His272 is expressed with a Fc, 6His tag at the C-terminus.

Background: R-spondin-3 (RSPO3), also known as Protein with TSP type-1 repeat, Roof plate-specific spondin-3, Thrombospondin type-1 domain-containing protein 2, PWTSR, THSD2 and CRISTIN1, is a member of the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein and widely expressed in many tissues. RSPO3 contains two Furin-like repeats which have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases, and one TSP type-1 domain, RSPO3 founctions as a activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Otherwise, RSPO3 may negatively regulate the TGF-beta pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 18490982
• NCBI GeneID: 84870
• NCBI Accession #: NP_116173.2
• NCBI GenBank Nucleotide #: NM_032784.5
• UniProt Accession #: Q9BXY4; Q5VTV4; Q96K87; B2RC27
• Molecular Weight: Molecular Mass: 56.3kDa; Actual Protein Molecular Mass: 61kDa
• NCBI Official Full Name: R-spondin-3
• NCBI Official Synonym Full Names: R-spondin 3
• NCBI Official Symbol: RSPO3
• NCBI Official Synonym Symbols: PWTSR; THSD2; CRISTIN1
• NCBI Protein Information: R-spondin-3
• UniProt Protein Name: R-spondin-3
• Protein Family: R-spondin
• UniProt Gene Name: RSPO3
• UniProt Synonym Gene Names: PWTSR; THSD2; hPWTSR; hRspo3

NCBI Description

This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]

Uniprot Description

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway (PubMed:21727895, PubMed:21909076, PubMed:22615920). Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells () (PubMed:21727895, PubMed:21909076, PubMed:22615920). Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3 (PubMed:29769720).