Human R-spondin-1 ELISA Kit | RSPO1 elisa kit

Human R-spondin-1 ELISA Kit

Cat. Num. AAA761485

• Gene Names: RSPO1; RSPO; CRISTIN3
• Reactivity: Human
• Synonyms: R-spondin-1; Human R-spondin-1 ELISA Kit; Cristin 3/RSPO1/CRISTIN3/FLJ40906Roof plate-specific spondin-1/hRspo1/roof plate-specific spondin/RSPO/RSPONDIN/R-spondin homolog (Xenopus laevis)/R-spondin1/R-spondin-1; RSPO1 elisa kit

• Reactivity: Human
• Sequence Length: 263

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RSPO1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human RSPO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RSPO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RSPO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RSPO1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 339276005
• NCBI GeneID: 284654
• NCBI Accession #: NP_001229837.1
• NCBI GenBank Nucleotide #: NM_001242908.1
• Molecular Weight: 21,775 Da
• NCBI Official Full Name: R-spondin-1 isoform 1
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: RSPO1
• NCBI Official Synonym Symbols: RSPO; CRISTIN3
• NCBI Protein Information: R-spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: RSPO1
• UniProt Synonym Gene Names: hRspo1
• UniProt Entry Name: RSPO1_HUMAN

NCBI Description

This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell adhesion; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: extracellular region; extracellular space; nucleus

Molecular Function: G-protein-coupled receptor binding; heparin binding; protein binding; receptor binding

Biological Process: male meiosis; positive regulation of protein amino acid phosphorylation; positive regulation of Wnt receptor signaling pathway; regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin

Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal