Rabbit anti-Human, Mouse EFEMP1 Polyclonal Antibody | anti-EFEMP1 antibody

EFEMP1, NT (EFEMP1, FBLN3, FBNL, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3) (PE)

Cat. Num. AAA6294532

• Gene Names: EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EFEMP1; Polyclonal Antibody; NT (EFEMP1; FBLN3; FBNL; EGF-containing fibulin-like extracellular matrix protein 1; Extracellular protein S1-5; Fibrillin-like protein; Fibulin-3) (PE); Fibulin-3; anti-EFEMP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EFEMP1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-EFEMP1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 119-148 in the N-terminal region of human EFEMP1. Species sequence homology: monkey and rat
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse lung tissue lysates (35ug/lane) usingMBS6000593. EFEMP1 (arrow) was detected usingMBS6000593.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human lung carcinoma usingMBS6000593, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6000593 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of NCI-H460 cells (bottom histogram) using MBS6000593 compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EFEMP1 antibody

Antibodies; Fibrillin

References

1. Okada, Y., et.al., Hum. Mol. Genet. (2010). In press 2. Wakabayashi, T., et.al., Biochem. Biophys. Res. Commun. 391 (1), 1116-1121 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 86788015
• NCBI GeneID: 2202
• NCBI Accession #: NP_001034437.1
• NCBI GenBank Nucleotide #: NM_001039348.2
• UniProt Accession #: Q12805; Q541U7; A8K3I4; D6W5D2
• Molecular Weight: 54,641 Da
• NCBI Official Full Name: EGF-containing fibulin-like extracellular matrix protein 1
• NCBI Official Synonym Full Names: EGF containing fibulin-like extracellular matrix protein 1
• NCBI Official Symbol: EFEMP1
• NCBI Official Synonym Symbols: DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3
• NCBI Protein Information: EGF-containing fibulin-like extracellular matrix protein 1; fibulin-3; extracellular protein S1-5
• UniProt Protein Name: EGF-containing fibulin-like extracellular matrix protein 1
• Protein Family: EGF-containing fibulin-like extracellular matrix protein
• UniProt Gene Name: EFEMP1
• UniProt Synonym Gene Names: FBLN3; FBNL; FIBL-3
• UniProt Entry Name: FBLN3_HUMAN

NCBI Description

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

Uniprot Description

EFEMP1: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD); also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p16

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: protein binding; growth factor activity; epidermal growth factor receptor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; visual perception; negative regulation of chondrocyte differentiation

Disease: Doyne Honeycomb Retinal Dystrophy