Mouse Ribonucleoside-diphosphate reductase large subunit ELISA Kit | Rrm1 elisa kit

Mouse Ribonucleoside-diphosphate reductase large subunit ELISA Kit

Cat. Num. AAA289833

• Gene Names: Rrm1; RnrM1
• Reactivity: Mouse
• Synonyms: Ribonucleoside-diphosphate reductase large subunit; Mouse Ribonucleoside-diphosphate reductase large subunit ELISA Kit; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit; Rrm1; 1.17.4.1; Rrm1 elisa kit

• Reactivity: Mouse
• Sequence Length: 792

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.091 ng/mL
• Intra-Assay CV: <=4.2%
• Inter-Assay CV: <=5.9%
• Recovery: 88%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 31982026
• NCBI GeneID: 20133
• NCBI Accession #: NP_033129.2
• NCBI GenBank Nucleotide #: NM_009103.3
• UniProt Accession #: P07742; Q91YM8
• Molecular Weight: 90,210 Da
• NCBI Official Full Name: ribonucleoside-diphosphate reductase large subunit
• NCBI Official Synonym Full Names: ribonucleotide reductase M1
• NCBI Official Symbol: Rrm1
• NCBI Official Synonym Symbols: RnrM1
• NCBI Protein Information: ribonucleoside-diphosphate reductase large subunit
• UniProt Protein Name: Ribonucleoside-diphosphate reductase large subunit
• Protein Family: Ribonucleoside-diphosphate reductase
• UniProt Gene Name: Rrm1
• UniProt Entry Name: RIR1_MOUSE

Uniprot Description

RRM1: an enzyme involved in DNA replication that provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Belongs to the ribonucleoside diphosphate reductase large chain family. Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the M1 subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site. Two distinct regulatory sites have been defined: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2. The level of the enzyme activity is closely correlated with the growth rate of a cell and appears to vary with the cell cycle. Patients with advanced non-small cell lung cancer responded favorably to gemcitabine.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; EC 1.17.4.1; Other Amino Acids Metabolism - glutathione; Oxidoreductase; DNA replication

Cellular Component: cell projection; cell soma; cytoplasm; nuclear envelope; ribonucleoside-diphosphate reductase complex

Molecular Function: ATP binding; catalytic activity; nucleotide binding; oxidoreductase activity; protein binding; purine nucleotide binding; ribonucleoside-diphosphate reductase activity

Biological Process: deoxyribonucleotide biosynthetic process; DNA replication; metabolic process; protein heterotetramerization; protein oligomerization